RESUMO
Enhanced efficiency for generating molecular ions is essential for high-throughput and sensitive detection using mass spectrometry in clinical diagnostics and biomarker discovery. In this study, we developed a novel strategy to promote laser desorption and ionization by using photonic crystals as substrates. The WO3-TiO2 inverse opal photonic crystal, with a coupling stop band and laser wavelength, significantly enhanced the efficiency of laser desorption and ionization owing to the slow light effect and the porous structure of the inverse opal, which increased the interaction between the laser and WO3-TiO2. Furthermore, stress biomarkers were conveniently measured under atmospheric pressure by using WO3-TiO2 inverse opal as an enhanced substrate to evaluate the impact of chronic unpredictable mild stress. The universal and highly sensitive substrate has promised for application in the highly sensitive detection and quantification of biomarkers.
Assuntos
Biomarcadores/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Animais , Pressão Atmosférica , Biomarcadores/sangue , Transtorno Depressivo/diagnóstico , Modelos Animais de Doenças , Luz , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Óxidos/química , Porosidade , Serotonina/sangue , Estresse Fisiológico , Estresse Psicológico , Titânio/química , Tungstênio/químicaRESUMO
A circlular structural-colored contact lens is reported, which is fabricated by replicating self-assembled colloidal photonic crystal templates. The structural-colored contact lenses not only display variable and brilliant color under light illumination, but also avoid the addition of any colorants to the hydrogel lenses and prevent the potential harm posed by traditional colored contact lenses.
