Strategy to synthesize dual-emission carbon dots and their application for pH variation and hydrogen sulfide sensing and bioimaging.

In this work, dual emission nitrogen and sulfur co-doped fluorescent carbon dots (DE-CDs) were designed for pH variation and hydrogen sulfide (H2S) sensing and bioimaging through fluorescence enhancement. The DE-CDs with green-orange emission were facilely prepared by one-pot hydrothermal strategy using neutral red and sodium 1,4-dinitrobenzene sulfonate as precursors, manifesting intriguing dual-emission behavior at 502 and 562 nm. As the pH increases from 2.0 to 10.2, the fluorescence of DE-CDs gradually increases. The linear ranges are 2.0-3.0 and 5.4-9.6, respectively, which are attributed to the abundant amino groups on the surface of the DE-CDs. Meanwhile, H2S can be employed as an enhancer to increase the fluorescence of DE-CDs. The linear range is 25-500 µM, and the LOD is calculated to be 9.7 µM. Besides, the DE-CDs can be used as imaging agents for pH variation and H2S sensing in living cells and zebrafish due to their low toxicity and good biocompatibility. All of the results demonstrated that the DE-CDs can monitor pH fluctuations and H2S in aqueous and biological environments, and have promising applications in the fields of fluorescence sensing, disease detection, and bioimaging.

Strategy to synthesize long-wavelength emission carbon dots and their multifunctional application for pH variation and arginine sensing and bioimaging.

In this work, we designed N and S co-doped carbon dots (N,S-CDs) with long-wavelength emission and their multifunctional application in pH variation, arginine (Arg) sensing, bioimaging in living cells and zebrafish, and fluorescent materials. The N,S-CDs with excitation wavelength-dependent properties were prepared using neutral red (NR) and dl-methionine (DL-Met) as raw materials by one-pot hydrothermal strategy. The N,S-CDs exhibited a unique pH-sensitive luminescence trait within pH range of 3.2-11.0 and have great linear relationship of 4.8-8.0, which indicating their potential application as an imaging reagent in physiological environments. Arg can quench the PL of N,S-CDs due to static quenching. (SQ). The linear range is 2.5-62.5 µM and the LOD is calculated as 0.68 µM. Furthermore, the as-proposed N,S-CDs can be applied as imaging reagents for monitoring of pH and Arg in vivo and vitro owing to outstanding biocompatibility and low cytotoxicity. Interestingly, the N,S-CDs were also used in fluorescent composite films and phosphors owing to exceptional optical properties. All these results indicate that the N,S-CDs have huge potentiality in the areas of fluorescence sensing, bioimaging and fluorescent materials.

The association between rotavirus diarrhea in children and atmospheric pressure in Guangzhou City from 2012 to 2020 / 中国学校卫生

Objective@#To explore the relative risks of rotavirus diarrhea after exposure to different levels of atmospheric pressure in children in Guangzhou City, so as to provide reference for improving public awareness of high atmospheric pressure exposure.@*Methods@#The study used the China Notifiable Communicable Diseases Network System and China Meteorological Science Data Sharing Service Network to collect meteorological data and data relating to daily cases of rotavirus diarrhea in children at Guangzhou Women and Children s Medical Center from 2012 to 2020. The association between rotavirus diarrhea and atmospheric pressure was analyzed using distributed lag non linear models (DLNM). The relative risks of different sex and age sub groups exposed to different atmospheric pressures were also evaluated.@*Results@#A total of 18 587 cases of rotavirus diarrhea were reported from 2012 to 2020, among which 11 662 cases (62.7%) were boys, and 12 582 cases (67.7%) were children aged 6 to 24 months old, which represented the highest proportion. The results of the DLNM showed that the relative risk of rotavirus diarrhea was the highest on the day of exposure to extreme high atmospheric pressure ( RR =1.50, 95% CI =1.24-1.82, P <0.05) and the effect could last for 28 days. Risk of rotavirus diarrhea was low for exposure to low pressure within 2 weeks ( P <0.05). During extremely high atmospheric pressure weather, RR was higher in girls ( RR =3.31, 95% CI =1.46-7.49， P <0.05) than that in boys ( RR =1.98, 95% CI =0.96-4.07， P >0.05). Among different age sub groups, RR was the highest in children aged 24 to 60 months after exposure to the highest level of atmospheric pressure exposure ( RR =3.36, 95% CI =1.27-8.89, P <0.05).@*Conclusion@#In Guangzhou, exposure to high pressure increases the risk of rotavirus diarrhea in children. In the future, public awareness should be raised regarding the risk after exposure to high atmospheric pressure.

Characteristics of Nosocomial Infections in Children Screened for SARS-CoV-2 Infection in China.

BACKGROUND This study summarizes the characteristics of children screened for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and reports the case of 1 child who was diagnosed with SARS-CoV-2 infection in Guangzhou Women and Children's Medical Center and the cases of his family members. MATERIAL AND METHODS The medical records of 159 children who were admitted to our hospital from January 23 to March 20, 2020, were retrospectively analyzed. Samples from pharyngeal or/and anal swabs were subjected to reverse-transcription polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 within 12 h of patient admission; a second RT-PCR test was done 24 h after the first test. RESULTS Of the 159 patients, 151 patients had epidemiological histories, 14 patients had cluster onset, and 8 patients had no epidemiological history but had symptoms similar to coronavirus disease 2019 (COVID-19). The most common symptom was fever (n=125), followed by respiratory and gastrointestinal symptoms. A 7-year-old boy in a cluster family from Wuhan was confirmed with asymptomatic SARS-CoV-2 infection with ground-glass opacity shadows on his lung computed tomography scan, and his swab RT-PCR test had not turned negative until day 19 of his hospitalization. In patients who did not test positive for SARS-CoV-2, influenza, respiratory syncytial virus, and adenovirus were observed. A total of 158 patients recovered, were discharged, and experienced no abnormalities during follow-up. CONCLUSIONS For SARS-CoV-2 nosocomial infections, taking a "standard prevention & contact isolation & droplet isolation & air isolation" strategy can prevent infection effectively. Children with clustered disease need close monitoring.

Characteristics of pediatric SARS-CoV-2 infection and potential evidence for persistent fecal viral shedding.

We report epidemiological and clinical investigations on ten pediatric SARS-CoV-2 infection cases confirmed by real-time reverse transcription PCR assay of SARS-CoV-2 RNA. Symptoms in these cases were nonspecific and no children required respiratory support or intensive care. Chest X-rays lacked definite signs of pneumonia, a defining feature of the infection in adult cases. Notably, eight children persistently tested positive on rectal swabs even after nasopharyngeal testing was negative, raising the possibility of fecal-oral transmission.

Wnt/Beta-Catenin Signaling and Prostate Cancer Therapy Resistance.

Metastatic or locally advanced prostate cancer (PCa) is typically treated with androgen deprivation therapy (ADT). Initially, PCa responds to the treatment and regresses. However, PCa almost always develops resistance to androgen deprivation and progresses to castrate-resistant prostate cancer (CRPCa), a currently incurable form of PCa. Wnt/ß-Catenin signaling is frequently activated in late stage PCa and contributes to the development of therapy resistance. Although activating mutations in the Wnt/ß-Catenin pathway are not common in primary PCa, this signaling cascade can be activated through other mechanisms in late stage PCa, including cross talk with other signaling pathways, growth factors and cytokines produced by the damaged tumor microenvironment, release of the co-activator ß-Catenin from sequestration after inhibition of androgen receptor (AR) signaling, altered expression of Wnt ligands and factors that modulate the Wnt signaling, and therapy-induced cellular senescence. Research from genetically engineered mouse models indicates that activation of Wnt/ß-Catenin signaling in the prostate is oncogenic, enables castrate-resistant PCa growth, induces an epithelial-to-mesenchymal transition (EMT), promotes neuroendocrine (NE) differentiation, and confers stem cell-like features to PCa cells. These important roles of Wnt/ß-Catenin signaling in PCa progression underscore the need for the development of drugs targeting this pathway to treat therapy-resistant PCa.

The effects of meteorological factors on influenza among children in Guangzhou, China.

BACKGROUND: Influenza seriously affects the health of children, yet little evidence is available on the association between meteorological factors and the occurrence of influenza among children in subtropical regions. The current study aimed to explore the effects of meteorological factors on influenza among children in Guangzhou, a subtropical city in China. METHODS: The distributed lag nonlinear model (DLNM) was used to assess the effects of meteorological factors on children influenza occurrence in Guangzhou, China. Daily number of influenza cases among children aged 0-17 years from 2013 to 2017 were obtained from the National Information System for Disease Control and Prevention. RESULTS: Mean temperature, relative humidity, and atmospheric pressure were associated with influenza cases. The relative risks (RRs) increased as temperature fell below 20°C. The relationship between relative humidity and influenza cases could be described with a U-shaped curve, and the RRs increased if relative humidity was lower than 50% or higher than 80%. The risk of influenza increased with rising atmospheric pressure with 1005 hPa as the break point. The cold effect, humid effect, dry effect, high-pressure effect, and low-pressure effect showed statistical significance both in female and male. The cold effect increased with age. The humid-effect affects all age ranges of children, but dry effect mainly affected 4-14 years old. High-pressure effect mainly affected the 0-3 years old, whereas low-pressure effect protected preschool children aged 0-6 years old. CONCLUSION: Mean temperature, relative humidity, and atmospheric pressure might be important predictors of the influenza occurrence among children in Guangzhou.

The Role of Maternal HP1a in Early Drosophila Embryogenesis via Regulation of Maternal Transcript Production.

Heterochromatin protein 1a (HP1a) is a highly conserved and versatile epigenetic factor that can both silence and activate transcription. However, the function of HP1a in development has been underinvestigated. Here, we report the role of maternal HP1a in producing maternal transcripts that drive early Drosophila embryogenesis. Maternal HP1a upregulates genes involved in translation, mRNA splicing, and cell division, but downregulates genes involved in neurogenesis, organogenesis, and germline development, which all occur later in development. Our study reveals the earliest contribution of HP1a during oogenesis in regulating the production of maternal transcripts that drive early Drosophila embryogenesis.

Pancreatic stellate cell secreted IL-6 stimulates STAT3 dependent invasiveness of pancreatic intraepithelial neoplasia and cancer cells.

Pancreatic ductal adenocarcinoma (PDAC) is a dynamic tumor supported by several stromal elements such as pancreatic stellate cells (PSC). Significant crosstalk exists between PSCs and tumor cells to stimulate oncogenic signaling and malignant progression of PDAC. However, how PSCs activate intercellular signaling in PDAC cells remains to be elucidated. We have previously shown that activated signal transducer and activator of transcription 3 (STAT3) signaling is a key component in the progression of pancreatic neoplasia. We hypothesize that PSC secreted IL-6 activates STAT3 signaling to promote PanIN progression to PDAC. Human PDAC and mouse PanIN cells were treated with PSC-conditioned media (PSC-CM), and phospho- and total-STAT3 levels by immunoblot analysis were determined. IL-6 was quantified in PSC-CM and cell invasion and colony formation assays were performed in the presence or absence of a neutralizing IL-6 antibody and the JAK/STAT3 inhibitor AZD1480. Serum from Ptf1aCre/+;LSL-KrasG12D/+;Tgfbr2flox/flox (PKT) and LSL-KrasG12D/+; Trp53R172H/+; Pdx1Cre/+ (KPC) mice demonstrated increased levels of IL-6 compared to serum from non-PDAC bearing KC and PK mice. PSC secreted IL-6 activated STAT3 signaling in noninvasive, precursor PanIN cells as well as PDAC cells, resulting in enhanced cell invasion and colony formation in both cell types. There was a significant positive linear correlation between IL-6 concentration and the ratio of phosphorylated STAT3/total STAT3. IL-6 neutralization or STAT3 inhibition attenuated PSC-CM induced activation of STAT3 signaling and tumorigenicity. These data provide evidence that PSCs are directly involved in promoting the progression of PanINs towards invasive carcinoma. This study demonstrates a novel role of PSC secreted IL-6 in transitioning noninvasive pancreatic precursor cells into invasive PDAC through the activation of STAT3 signaling.

Relationships between weight status and bullying victimization among school-aged adolescents in Guangdong Province of China.

OBJECTIVE: To examine relationships between weight status and different forms of bullying victimization among adolescents aged 11-18 years. METHODS: The relationships between weight status and bullying victimization (physical, verbal, and relational) were examined utilizing data from the Guangdong Provincial Youth Health Behavior Survey. Data on height, weight, and victimization behaviors were collected by self-reporting from 12 439 subjects. , test and logistic regression were used to analyze relationships between weight and bullying victimization. RESULTS: The incidence of victimization for adolescents aged 11-18 years was 8.6%, with higher rates for boys (12.4%) than for girls (4.7%). For children with normal, overweight and obese body mass index (BMI), the incidence rates of victimization were 8.2%, 17.3%, and 11.5%, respectively. Compared to normal weight, overweight was a risk factor for bullying victimization(OR = 1.60, 95% CI: 1.18-2.17), and it also increased children's risk of being teased in a hurtful way (OR = 2.13, 95% CI: 1.41-3.24) and being made fun of due to physical appearance (OR = 3.58, 95% CI: 2.27-5.67). Obesity only increased the risk for children of being made fun of due to physical appearance (OR = 2.45, 95% CI: 1.44-4.15). CONCLUSIONS: The victimization for children at school is common in Guangdong province, China. Overweight and obese children are more likely to be victims of bullying behaviors, especially verbal victimization.

[Evaluation on the health education program regarding prevention of non-fatal drowning among school-aged children in Lianping county, Guangdong province].

OBJECTIVE: To evaluate the outcome of health education program on drowning prevention among primary and secondary school children in rural areas. METHODS: A township was selected and all the students from grade 3 to 5, grade 7 to 8, and grade 10 to 11 were selected to take part in the program. Twelve intervention measures on natural water safety and drowning prevention were carried out for one year. Information was collected using the same questionnaire before and after the intervention program. RESULTS: One year after the intervention was carried out, children's knowledge on drowning prevention improved significantly (13.21, 95%CI: 12.51 - 13.90), and a positive effect was also noticed among boys (12.77, 95%CI: 11.77 - 13.77), girls (13.80, 95%CI: 12.82 - 14.78), and among primary school children(15.51, 95%CI: 14.30 - 16.72), senior high school children (10.78, 95%CI: 9.50 - 12.05) and junior high school children (12.77, 95%CI: 11.84 - 13.71). Overall rates on risk behaviors dropped from 41.4% to 32.2% (by 22.2%) including 15.6% for boys, 35.2% for girls and 13.8%, 29.3%, 26.3% for primary school children, senior high school children, junior high school children, respectively. The incidence rates for non-fatal drowning decreased by 58.9% (from 5.6% to 2.3%). The person-times for treatment on sight, in emergency settings, in outpatient clinic or in the hospitals had a reduction from 399, 78, 36 to 175, 32, 14, respectively. CONCLUSION: Health education program could improve children's perception on water safety, and reduce their risk behaviors as well as on the incidence of non-fatal drowning in the rural areas.