RESUMO
Studies have shown that oleuropein has antifungal, antiinflammatory, antiviral, antioxidant, anticancer and hypoglycemic functions. TTC solution staining was used to measure myocardial infarction size. A commercial kit was used to measure lactate dehydrogenase (LDH), creatinine kinaseMB (CKMB), tumor necrosis factorα (TNFα), interleukin1ß (IL1ß), IL 6, superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA) and catalase levels. Western blot analysis was used to measure p53, p-MEK p-ERK and pIκBα protein expression. The present study reports that the protective effect of oleuropein also prevents against myocardial ischemia/reperfusion (myocardial I/R). The aim of this retrospective study was to evaluate this protective effect of oleuropein and the mechanisms by which myocardial I/R is prevented. Oleuropein inhibited myocardial infarction size, CKMB and LDH serum levels in a myocardial I/R rat model. Moreover, oleuropein also attenuated caspase3 activity, and p53, phosphorylated (p)mitogenactivated protein kinase kinase (MEK), pextracellular signalregulated protein kinase (ERK) and pIκBα protein expression. TNFα, IL1ß, IL6 and MDA were decreased; SOD, GSH and catalase levels inhibited TNFα, IL1ß, IL-6 and MDA levels, and increased SOD, GSH and catalase levels in myocardial I/R rats treated with oleuropein. Rats orally administered the MEK inhibitor PD0325901, in addition to oleuropein, exhibited inhibited myocardial infarction size, CKMB and LDH serum levels compared with rats treated with oleuropein only. Rats treated with MEK inhibitor also exhibited suppressed caspase3 activity, p53, pMEK pERK and pIκBα protein expression, TNFα, IL1ß, IL6, SOD, GSH, MDA and catalase levels, and induced psignal transducer and activator of transcription 3 (STAT3) protein expression compared with rats treated with oleuropein only. Taken together, these results suggest that MEK/ERK/STAT3 signaling regulates the inhibition of myocardial I/R in rats treated with oleuropein.
