Study on border patrol task planning of heterogeneous UAVs group based on swarm intelligence.

Heterogeneous UAVs performing patrol tasks are a new type of border patrol method with high flexibility, high patrol efficiency, and low operating cost In order to improve the ability of heterogeneous UAVs to perform border patrol task, by constructing a three-dimensional complex coordinated planning model, a multi-objective fitness function with the minimum patrol energy consumption and maximum patrol coverage of UAVs in a complex mountain environment is established. Design an improved shuffled frog leaping algorithm (ISFLA) based on spiral search mechanism to solve the problem of task planning in complex mountain environment. The proposed algorithm is verified by simulation experiments. The simulation results show that the ISFLA algorithm for solving the path problem in complex three-dimensional environment has significantly improved the solving efficiency, accuracy and global convergence compared with the particle swarm optimization (PSO), differential evolution (DE) and shuffled frog leaping algorithm (SFLA). The experiments show that the proposed algorithm also has excellent solving ability in solving complex planning problems.

High-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response.

A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC). The high-throughput screening system was used to cultivate forty-three bacterial enrichments and gained a halophilic bacterial community E3 with the best phenol-degrading capability. Halomonas sp. strain 4-5 was isolated from the E3 community. Strain 4-5 was able to degrade more than 94% of the phenol (500 mg · L(-1) starting concentration) over a range of 3%-10% NaCl. Additionally, the strain accumulated the compatible solute, ectoine, with increasing salt concentrations. PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.