Anthropogenic activities drive the distribution and ecological risk of antibiotics in a highly urbanized river basin.

Although antibiotics are widely detected in river water, their quantitative relationships with influencing factors in rivers remain largely unexplored. Here, 15 widely used antibiotics were comprehensively analyzed in the Dongjiang River of the Pearl River system. The total antibiotic concentration in river water ranged from 13.84 to 475.04 ng/L, with fluoroquinolones increasing from 11 % in the upstream to 38 % in the downstream. The total antibiotic concentration was high downstream and significantly correlated with the spatial distribution of population density, animal production, and land-use type. The total risk quotient of antibiotics for algae was higher than that for crustaceans and fish. Based on the optimized risk quotient method, amoxicillin, ofloxacin, and norfloxacin were identified as priority antibiotics. The key predictors of antibiotic levels were screened through Mantel test, correlation analysis, and multiple regression models. Water physicochemical parameters significantly impacted antibiotics and could be used as easy-to-measure surrogates associated with elevated antibiotics. Cropland negatively affected fluoroquinolones and sulfonamides, whereas urban land exerted positive impacts on fluoroquinolones, ß-lactam, and sulfonamides. In the main stream, population, animal production, urbanization status, and economic development had key effects on the distribution of florfenicol, norfloxacin, ofloxacin, and sulfadiazine.

Fine particle contents in sediment drive silica transport and deposition to the estuary in the turbid river basin.

Changes in riverine sediment transport are an important part of land-sea geochemical cycling and further impact geochemical element fluxes in turbid rivers. However, as a vital nutrient element supporting primary productivity, silica mobilization from drainage in turbid rivers is overlooked. The turbid Yellow River has a strong ability to adsorb reactive silica, thereby exerting a substantial impact on the estuarine deposition of silica. Through an integration of monitoring databases, field sampling and historical hydrological data, we concluded that riverine fine particles control the exchangeable silica in the river and its estuary under soil erosion. Indoor simulation further revealed that the adsorbed content of exchangeable silica (ex-Si) in fine sediment constituted 35 % of total sediment matter. In addition, the transport of phosphorus and ex-Si was jointly regulated by fine sediment in global fluvial sediment transport, thereby exerting additional influence on the trophic structure of estuarine ecosystems. Against the backdrop of sediment budget deficit in the estuary, the heightened content of fine particles is depleting the silica storage from estuarine sediments.

Nitrate losses from forest during snowmelt: An underestimated source in mid-high latitude watershed.

The forest nitrate cycle is a crucial part of the watershed nitrate load but has received limited attention compared to that of agricultural and residential land. Here, we analyzed the status and sources of riverine nitrate fluxes and identified the characteristics and contribution of forest nitrate loss to the riverine system in a mid-high latitude forested watershed using monthly field sampling and a modified Soil and Water Assessment Tool (SWAT) with enhanced forest nutrient cycle representation. The results indicate that nitrate losses in the headwater stream and downstream exhibit different seasonal characteristics. The nitrate losses in the headwater stream show a bimodal pattern due to lower temperatures and snowmelt runoff. Redundancy analysis (RDA) revealed that, unlike nitrogen (N) fertilizer-induced nitrate loss in the rainy season, forest loss has a positive effect on headwater stream nitrate concentration during the snowmelt season. The modified SWAT was then utilized to simulate nitrate losses in forest lands. The forest nitrate export per unit area of the headwater stream (1.58 ± 1.78 kg/ha/yr) was observed to be higher than that of the downstream (0.67 ± 0.74 kg/ha/yr) due to high snowmelt and mineralization of active organic N. At watershed scale, forest lands contributed 8.18 ± 3.94 % of the total nitrate losses to the water system in the headwater watersheds during the snowmelt season, representing the highest level within the entire basin. A comparison with forest streams in similar low-temperature conditions worldwide revealed that increasing nitrate loss occurred after extreme cold weather or soil freezing events, with an average increment of 6.32 kg/ha/yr. Therefore, forest nitrate losses should be better characterized and included in future watershed N budgets in low-temperature regions, which might help to reduce the N budget uncertainty and improve watershed management.

Occurrence, distribution, and potential ecological risks of antibiotics in a seasonal freeze-thaw basin.

Antibiotics have gained increasing attention as pharmaceuticals widely existing in human society. Under low temperature conditions, antibiotics tend to have higher environmental persistence, which poses a potential threat to ecological environment, but research on antibiotics in low-temperature basins is still lacking. Therefore, for investigating occurrence, spatio-temporal distributions, and ecological risks of antibiotics in a seasonal freeze-thaw basin, rivers in Tumen River basin were selected and sampled, including 25 samples during the river-freezing season and 27 samples during the non-freezing season. Overall, climate characteristics of different latitudes and renewal frequency of antibiotics are important factors that lead to diversity of antibiotics in basins. Eleven target antibiotics were detected and their average concentrations during the river-freezing season (0.83-27.5 ng L-1) were lower than that during the non-freezing season (2.80-45.30 ng L-1), severely impacted by river flow, ice sealed-melting, and local feeding practices. In addition, total antibiotic concentrations are usually highest in downstream areas of human settlements, receiving input from husbandry and sewage, respectively. Through ecological risk assessment, norfloxacin and amoxicillin posed high risks to algae, which were identified as high-risk pollutants in basin.

Industrial impacts on vanadium contamination in sediments of Chinese rivers and bays.

Vanadium, like many trace metals, is persistent and detrimental to ecosystems at elevated concentrations. Likewise, it is versatile, functional, and used in many industries. Jiaozhou Bay (JZB) and Laizhou Bay (LZB) are valuable coastal ecosystems in China coexisting with several of these vanadium-related industries; however, limited studies have been conducted regarding vanadium occurrence, distribution, sources and risks in sediments. 208 surface sediment samples were collected from rivers and bays over two years and analyzed using inductively coupled plasma optical emission spectrometry. Overall, sediments near vanadium-related industries have significantly higher vanadium concentrations than those near traditional industries, with 30.3% and 22.9% higher average concentrations of vanadium in sediments of JZB and LZB, respectively. Vanadium accumulation at LZB is positively correlated with fine sediment, oxides (e.g., Fe, Ti, Mn), and organic matter content, while temporal changes in parts of JZB highlight the impacts of oxides, pH, and redox conditions on its accumulation. After geochemical normalization, the concentrations in marine samples from LZB showed slightly polluted sediments under the Modified Nemerow pollution index. Likewise, the elevated concentrations of vanadium in JZB, rivers and bay, were classified as slightly polluted and correlated with anthropogenic activities, such as the coal and petrochemical industries. Temporal changes indicated higher enrichments in 2019. Last, humans could be responsible for up to 46.8% and 16.2% of the vanadium accumulation in JZB and LZB, respectively, yet risks to species remain limited.

Watershed seasonality regulating vanadium concentrations and ecological risks in the coastal aquatic habitats of the northwest Pacific.

Vanadium is a component of different natural and industrial products and a widely used metal, which, nonetheless, has only garnered attention in recent years owing to its potential risks. Six sampling trips were conducted over different seasons and years, collecting 108 samples from rivers and 232 from the bays and analyzed using high-precision inductively coupled plasma mass spectrometry. This study investigated the sources, spatiotemporal characteristics, and risks of vanadium in the aquatic ecosystems of two typical bays of the Northwest Pacific that have strong links with vanadium-related industries. Likewise, the health and ecological risks were assessed using probabilistic and deterministic approaches. Overall, vanadium concentrations were higher in Jiaozhou Bay (JZB: 0.41-52.7 µg L-1) than in Laizhou Bay (LZB: 0.39-17.27 µg L-1), with concentrations higher than the majority of the worldwide studies. Vanadium-realted industries significantly impacted (p < 0.05) the metal concentrations in the rivers with 54.22% (40.73-150%) and 54.45% (27.66%-68.87%) greater concentrations in JZB and LZB rivers. In addition, vanadium exhibited significant seasonal variation, and higher values were quantified during the monsoon period at LZB owing to the greater catchment area. Impacted by smaller freshwater inputs, the post-monsoon period had substantial impacts on JZB, and vanadium in the rivers and bays was significantly higher during the winter. Despite some concentrations being higher than that indicated in the drinking water guidelines established by China, vanadium presents low to null risks to the population as per both approaches. Last, species with limited resilience are likely to face medium to high risks, with an incidence of 65-93% using the probabilistic method and 52-97% using the deterministic assessment.

Occurrence, spatiotemporal dynamics, and ecological risk of fungicides in a reservoir-regulated basin.

As an indispensable type of pesticide, fungicides have been somewhat neglected compared to insecticides and herbicides. Heavy fungicide application in agricultural regions may generate downstream ecological concerns via in-stream transport, and the reservoir complicates the process. Monitoring fungicide exposure and exploring reservoir effect on fungicide transport is the key to develop the downstream strategies of agricultural diffusion pollution control. Here, we investigated the exposure, spatiotemporal dynamics, and ecological risk of fungicides in a reservoir-regulated agricultural basin, located in the middle of the Yangtze River Basin, China. Seven fungicides were preliminarily identified and exhibited high detection frequencies (>85 %) in subsequent quantification of water samples from three sampling activities. The total concentration of fungicides ranged from 2.47 to 560.29 ng/L, 28.35 to 274.69 ng/L, and 13.61 to 146.968 ng/L in April, September, and November, respectively. Overall, the contamination levels of fungicides were in the ascending order of April < November < September. The spatial distribution of fungicides was closely associated with the dense of cultivated land, supporting its agricultural source. Furthermore, the reservoir plays a retention role in fungicides, alleviating ecological pressure downstream during the water storage period. Yet, due to the alternation of "source" and "sink" function of the reservoir, the contribution of Zijiang River to the fungicide load in the Yangtze River Basin still needs further attention. Although there is no acute risk posed by fungicides, even in the high-exposure scenario, the chronic ecological risk could not be ignored. Agricultural intensive regions, coupled with the reservoir, provide rather substantial chronic ecological concerns. Carbendazim has been designated as a priority pollutant that contributes significantly to cumulative chronic risk. Thus, we emphasize strengthening the supervision of fungicides in surface water and rationally restricting the use of carbendazim in agricultural operations.

Gradient of suspended particulate matter hastens the multi-interface partition dynamics of atrazine and its degradation products.

Herbicides are ubiquitous pollutants in estuaries because of the increased demand for food and the need for intensive agricultural systems worldwide. Multi-interface partitioning processes are inadequate for the degradation products of herbicides, especially in sediment-laden river estuaries with intensive water and sediment partitioning. Therefore, the partition characteristics of atrazine and its degradation products at the surface water-suspended particulate matter (SPM), surface water-surface sediment, and SPM-surface sediment interfaces in a typical sediment-laden river estuary were analyzed, the dominant environmental factors were described, and the related mechanisms were explored. The results showed that the partitioning priority of atrazine and its degradation products was surface water > SPM > surface sediment. The partition coefficients of these three interfaces were significantly correlated. The primary degradation products and desisopropylhydroxyatrazine tended to partition into the SPM, and desethyldesisopropylatrazine tended to partition into the surface sediment. Canonical analysis and structural equation modeling indicated that temperature, salinity, sediment pH, and SPM concentrations were the main influencing factors. In the sediment-laden river estuary, the SPM concentration was the most dominant factor. The partition coefficients increased exponentially when the SPM concentration was <150 mg/L at the SPM-surface sediment interface, leading to a rapid shift in the interface proportion of atrazine and its degradation products. In the context of climate change and human activities, the SPM concentration in the estuary was decreasing, which hastened the release potential for atrazine and its degradation products from the estuarine sediment. The investigation of the partition traits of organic degradation pollutants promotes the understanding of the multi-interface transport processes in estuaries.

Spatiotemporal partition dynamics of typical herbicides at a turbid river estuary.

Organic pollutants are ubiquitous in estuarine areas, nonetheless, the transport mechanisms of herbicides in such areas are limited. Atrazine and acetochlor were analyzed in suspended particle matter (SPM), surface sediment, and surface water from the Yellow River estuary and the surrounding rivers and sea. Among these rivers, the Yellow River contributes the most herbicide flux to the sea annually. The herbicide concentrations in water and sediment decreased from the estuarine areas to the deep sea. The fugacity fraction values of atrazine exceeded 0.5 in the Yellow River estuary, which supported that the herbicides in sediment desorbed at the estuarine areas. The herbicide in the SPM showed high concentration in the outer sea and increased as a power function with decreasing SPM content. The increasing partition capacity indicated that the herbicides tended to sink into sediment, increasing the ecological risk posed by herbicides. The ecological risk of acetochlor deserves continuous attention.

Overexpression of Uric Acid Transporter SLC2A9 Inhibits Proliferation of Hepatocellular Carcinoma Cells.

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-associated mortality worldwide. Although the mechanisms of HCC progression are not well understood, recent studies demonstrated the potential contribution of uric acid transporter SLC2A9 to tumor suppression. However, the roles and underlying mechanisms are still unknown. We aimed to study the roles and mechanisms of SLC2A9 in HCC. The present study showed that SLC2A9 expression was decreased in human HCC tissues and cell lines. In addition, overexpression of SLC2A9 inhibited HCC cell proliferation. SCL2A9 induced HCC cell apoptosis by inhibiting the expression of caspase 3. Our study also revealed that upregulation of SLC2A9 reduced intracellular reactive oxygen species (ROS) accumulation. Furthermore, SLC2A9 increased the mRNA and protein expression of tumor suppressor p53 in HCC cells. Probenecid inhibits SLC2A9-mediated uric acid transport, which promotes cell proliferation, inhibits cell apoptosis, induces intracellular ROS, and decreases the expression of p53 in HCC cells. Therefore, the present study demonstrated that SLC2A9 may be a novel tumor suppressor gene and a potential therapeutic target in HCC.