Potentially important markers in thyroid neoplasia: Claudin-1 and MMP-7.

Background: Thyroid carcinomas are the most common malignant endocrine tumors, and various immunohistochemical markers are tested in routine practice to reduce diagnostic differences, as well as to elucidate carcinogenesis and detect malignancy. Disruption of basement membranes and the extracellular matrix is an important step in tumor carcinogenesis and progression. The claudin and matrix metalloproteinase families are also thought to be effective in this process. Aim: In this retrospective study, the comparative expression of claudin-1 and MMP-7 immunomarkers in normal tissues and thyroid neoplasia were investigated. Materials and Methods: Immunohistochemical staining was performed for claudin-1 and matrix metalloproteinase 7 (MMP-7) in 112 sections, including 24 follicular adenomas, 22 follicular carcinomas, 24 medullary carcinomas, 24 papillary carcinomas, and 18 single dominant nodules from thyroid lesions. Results: A significant staining difference for claudin-1 was observed in follicular carcinoma and medullary carcinoma, papillary carcinoma, and single dominant nodules compared to normal thyroid tissue. A statistically significant staining difference was observed for MMP-7 in follicular adenoma, medullary carcinoma, and papillary carcinoma compared to normal thyroid tissue. Conclusions: These results indicate that claudin-1 and MMP-7 are important in the diagnosis, differential diagnosis, and carcinogenesis of follicular adenoma, follicular carcinoma, medullary carcinoma, papillary carcinoma, and single dominant nodules.

The investigation of biochemical and microbiological properties of four different honey types produced in turkey and the comparison of their effects with silver sulfadiazine on wound healing in a rat model of burn injury.

BACKGROUND: To determine and compare the effects of different honey types on wound healing in an animal model, with silver sulfadiazine as the standard treatment. MATERIALS AND METHODS: Five different groups were created with eight rats in each group. Partial-depth burns were created, and different types of honey and silver sulfadiazine were applied to the respective groups. Rats were monitored for 21 days, and wound cultures were obtained. Histopathological evaluation and cytokine analysis of final tissue samples were performed. In addition, the biochemical and microbiological analyses of the four types of honey used in the study were performed. RESULTS: Wound shrinkage comparisons showed that all four honey-treated groups (Bingöl, Konya, cotton, and citrus) performed better than the silver sulfadiazine group (honey groups, respectively, 86.86%, 84.72%, 89.61%, and 95.33% vs. control 82.90%). However, only citrus honey caused a significant difference in wound shrinkage rate when compared with other groups as well with control group (95.34% vs. 82.9%, P < 0.05). In tissues, all honey groups had higher cytokine (interleukin [IL]-6, IL-1B, tumor necrosis factor [TNF]-α) values compared with controls (P < 0.001). Honey analysis showed a significant inverse relationship between Fe (iron) and the number of diastases. CONCLUSIONS: The results of this study support the role of honey in wound healing, due to its antibacterial and immunomodulatory effects. More studies are needed to identify the role of honey composition in wound healing.

Effect of the number of portal area on modified histological activity index of viral hepatitis and histological findings.

AIM: Viral hepatitis is one of the most important causes of chronic hepatitis. Liver biopsy is used to verify clinical diagnosis and to evaluate necroinflammation and fibrosis. Biopsy is the guide for therapy and can be performed also after treatment to assess the effect of therapy on liver. This paper aimed to explore histopathological characteristics of biopsy samples, which had been referred to our department with the clinical diagnosis of chronic viral hepatitis, in reference to Ishak Modified Hepatic Activity Index (IMHAI), as well as to compare inflammatory scores and stages in the groups created according to the number of portal area (PA). MATERIALS AND METHODS: The study included 107 patients that underwent liver biopsy in 2011 being diagnosed with chronic viral hepatitis. Age, gender and type of viral hepatitis were retrospectively reviewed and histological findings such as IMHAI inflammatory score and stage, hepatosteatosis and ground glass hepatocytes were re-assessed by two pathologists. RESULTS: Of the present cases, 97 had chronic hepatitis B, 5 had chronic hepatitis C, and 5 had chronic hepatitis BDThe group with PA number of 2-4 consisted of 8 cases and the group with PA number of 11 and over consisted of 37 cases. CONCLUSION: Statistical analysis performed by comparing IMHAI inflammatory score and stage with PA revealed that score and stage were significantly higher in PA ≥ 11 groups as compared to PA 2-4 group.