RESUMO
Tick-borne encephalitis (TBE) virus strains isolated in different geographic areas were characterized by changes in electrophoretic mobility (EPM) of virus-specific proteins. Analysis of EPM of intracellular virus-specific protein E and other high molecular proteins of 42 TBE virus strains revealed similarity among the majority of them, with the exception of several strains including the Sophyin strain (Far-Eastern subtype). The most marked variability in the electrophoretic behavior of virus-specific proteins was observed with low molecular proteins prM, ns4b, ns2a.
Assuntos
Vírus da Encefalite Transmitidos por Carrapatos/química , Proteínas Virais/análise , Animais , Comunidade dos Estados Independentes , Eletroforese em Gel de Poliacrilamida , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Humanos , Camundongos , Peso Molecular , Testes de Precipitina , Inoculações Seriadas , Carrapatos/microbiologia , Proteínas Virais/isolamento & purificaçãoRESUMO
Hybridization experiments with RNA of 143 tick-borne encephalitis (TBE) virus strains isolated in different parts of the distribution area were used to study the reactivity of kDNA- and a set of 10 synthetic deoxyoligonucleotide probes. The kDNA probe under certain conditions was shown to hybridize with RNA of all the strains under study, and under other (strict) hybridization conditions did so selectively with a small number of strains. The capacity of oligonucleotide probes for hybridization with RNA of TBE virus strains varied from 12% to 100%. The differences in the hybridization activity of kDNA- and oligonucleotide probes complementary to the genomes of the Sophyin strain (Far-Eastern subtype) and Neudorffle strain (Western subtype) with TBE virus strains were used for differentiation of the strains into six genetic variants. Comparison of the reactivity of molecular probes in experiments with RNA of TBE virus strains and viruses of the TBE complex showed that the differences of the strains belonging to different genetic variants from the prototype Sophyin strain were comparable to those of some members of the TBE complex, with the exception of Powassan virus. These data attest to the necessity of further studies dealing with specification of the taxonomy of TBE complex viruses.
Assuntos
Sondas de DNA/genética , DNA Viral/genética , DNA/genética , Vírus da Encefalite Transmitidos por Carrapatos/genética , Genoma Viral , Sondas de Oligonucleotídeos/genética , RNA Viral/genética , Animais , Sequência de Bases , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Variação Genética/genética , Dados de Sequência Molecular , Hibridização de Ácido Nucleico/métodos , RNA Viral/isolamento & purificação , Federação Russa , Carrapatos/microbiologiaRESUMO
Geographic distribution of 185 tick-borne encephalitis (TBE) virus strains isolated in 8 physico-geographic areas and classified into six genetic variants was analysed. The strains of genetic variant I homologous to the Sophyin prototype strain were found to occur predominantly in the Far East and also frequently found in Western and North-Western parts of the East European plain. The vast territories from lake Baikal in the East to Ukraine in the West harbor mostly the strains significantly different from the Far-Eastern Sophyin strain. Hybridization experiments with oligonucleotide probes specific for the Neudorffle strain showed that the strains genetically similar to the virus of central European encephalitis occurred also in Eastern Europe and Western Siberia. It is concluded that a relationship exists between genetic types of TBE virus and their geographic origin.
Assuntos
Vírus da Encefalite Transmitidos por Carrapatos/genética , Variação Genética/genética , Animais , Animais Recém-Nascidos , DNA/genética , Sondas de DNA/genética , DNA Viral/genética , Reservatórios de Doenças , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Genoma Viral , Camundongos , Sondas de Oligonucleotídeos/genética , RNA Viral/genética , Federação Russa , Carrapatos/microbiologiaRESUMO
The antigenic structure of 48 tick-borne encephalitis virus (TBE) strains isolated in the Irkutsk region and the Buryat ASSR in 1960-1981 was studied. Antigenic analysis was performed by the agar gel diffusion-precipitation test using adsorption of the immune sera. A new group of 31 TBE virus strains was found to belong to the Aina/1448 antigenic type. Close ecological relationship between this serotype and Muridae was found. Ix. persulcatus ticks are vectors of 2 TBE virus serotypes, Aina/1448 and eastern serotype. Data on combined circulation of these 2 serotypes of TBE virus in the Irkutsk region and the Buryat ASSR were obtained, the Aina/1448 serotype definitely dominating in 3 areas of the Irkutsk region. The areas of circulation of the Aina/1448 serotype strains are associated with forest and forest-steppe landscapes disturbed by human economic activity.
