RESUMO
Seven or 14 days of treatment with the beta 2-adrenoceptor agonist clenbuterol, 0.3 mg kg-1, s.c., twice daily, increased the basal mean blood pressure in normotensive urethane-anaesthetized rats. The elevated pressure values were maintained until 48 h after the end of the 14 day treatment. Clenbuterol treatment decreased the vasodilatory responses to the beta-adrenoceptor agonist isoprenaline and adenosine, agents which act through an increase in intracellular cyclic AMP. Decreased responses were maintained until 48 h after a 14 day treatment with clenbuterol. On the other hand, its administration to rats for 14 days did not modify the vasodilator responses to acetylcholine or sodium nitroprusside, two agents that exert their effects by enhancing cyclic GMP. The increase in mean blood pressure in urethane-anaesthetized rats after clenbuterol treatment may be a consequence of a reduced vasodilator beta 2-adrenoceptor-mediated response to circulating catecholamines.
Assuntos
Anestesia , Clembuterol/farmacologia , Uretana , Vasodilatadores/farmacologia , Acetilcolina/farmacologia , Adenosina/farmacologia , Animais , Pressão Sanguínea/efeitos dos fármacos , Nitroprussiato/farmacologia , Ratos , Ratos EndogâmicosRESUMO
The importance of the interaction of alpha- and beta-adrenoceptors in maintaining vascular tone in rats was studied. This interaction after clenbuterol (CLEN) treatment indicates an important contribution of the circulating epinephrine (EPI) levels. In urethane-anesthetized rats, the beta 2-adrenoceptor antagonist ICI 118.551 was more effective in antagonizing isoproterenol-induced hypotension (mainly beta 2-mediated) than tachycardia (mainly beta 1-mediated). Intravenous (i.v.) administration of the alpha 2-adrenoceptor agonist clonidine (CLO) induced an initial pressor response followed by a more prolonged hypotension and bradycardia. The initial hypertensive effect was potentiated by previous acute administration of ICI 118.551 as well as by the nonselective beta-adrenoceptor antagonist propranolol, but not by metoprolol, a more selective beta 1-blocker. Fourteen days of administration of the beta 2-adrenoceptor agonist CLEN [0.3 mg/kg, subcutaneously (s.c.) twice daily], a treatment that induces desensitization of beta 2-mediated vasodilation, increased the pressor response induced by CLO, an effect that was not observed in pentobarbital-anesthetized rats. In any case, neither beta-blockers nor CLEN treatment affects the hypotension and bradycardia induced by CLO. Mean blood pressure (BP) of CLEN-treated rats was increased under urethane anesthesia but not under pentobarbital anesthesia. Catecholamine levels (principally EPI) were higher in urethane-anesthetized rats. These results provide further evidence of a functional interaction between alpha 2- and beta 2-adrenoceptor-mediated responses in rat vasculature and suggest that vasodilator beta 2-adrenoceptors might contribute to the determination of peripheral vascular tone when circulating EPI is substantially elevated.
Assuntos
Músculo Liso Vascular/efeitos dos fármacos , Receptores Adrenérgicos alfa/efeitos dos fármacos , Receptores Adrenérgicos beta/efeitos dos fármacos , Vasoconstrição/efeitos dos fármacos , Vasodilatação/efeitos dos fármacos , Anestesia , Animais , Pressão Sanguínea/efeitos dos fármacos , Catecolaminas/sangue , Clembuterol/farmacologia , Frequência Cardíaca/efeitos dos fármacos , Isoproterenol/farmacologia , Masculino , Tono Muscular/efeitos dos fármacos , Propanolaminas/farmacologia , RatosRESUMO
Short-term treatment with clenbuterol [0.6 mg/kg-1 subcutaneously (s.c.) daily] produces a pressor effect in stressed rats after a period of immobilization (40 min). The stress applied markedly increases the plasma concentrations of norepinephrine (NE) and epinephrine. After bilateral adrenal demedullation, the increased levels of catecholamines and the hypertensive response obtained after clenbuterol treatment in the stressed animals were reduced to the values of the control rats. Clenbuterol treatment produced desensitization of the beta 2-adrenoceptor-mediated effect and thus reduced the vasodilator response induced by isoproterenol and increased the vasoconstriction produced by epinephrine but not that caused by NE. This desensitization may be responsible for the hypertensive response after clenbuterol treatment in stressed animals which is attenuated after adrenal demedullation. In conclusion, our results provide evidence that clenbuterol treatment induces pressor effect in normotensive animals under stress.
Assuntos
Pressão Sanguínea/efeitos dos fármacos , Clembuterol/farmacologia , Etanolaminas/farmacologia , Medula Suprarrenal/fisiologia , Animais , Catecolaminas/sangue , Epinefrina/sangue , Feminino , Frequência Cardíaca/efeitos dos fármacos , Técnicas In Vitro , Isoproterenol/farmacologia , Norepinefrina/sangue , Ratos , Ratos Endogâmicos , Restrição FísicaRESUMO
A study was made of the effect of several antidepressive drugs with different mechanisms of action on cardiovascular response induced by clonidine (CLO) or guanabenz (GUA) in anaesthetized normotensive rats. The acute pretreatment (1 mg.kg-1) with desipramine (DMI), maprotiline (MAP) and mianserin (MIA) reduced the hypotensive and bradycardiac responses induced by CLO (10 micrograms.kg-1), while only MIA prevented these central actions when elicited by GUA (30 micrograms.kg-1). Only chronic treatment with DMI reduced the hypotensive effect of CLO, but none of long-term antidepressant administrations decreased the fall in blood pressure induced by GUA. The long-term administration of DMI, MAP and MIA abolished the bradycardia from both agonists, CLO and GUA. The different pharmacological interaction of CLO and GUA with (acute or chronic) antidepressive treatments could be explained by a structural difference between the two agonists, and also by differences in localization and/or subtype of the alpha-adrenoceptors involved in their central cardiovascular effects.