RESUMO
Filtration of platelet-rich heparinized plasma deprived lymphocytes and contained lymphocytes was investigated in 21 donors. Filtration of both plasmas was performed through 5 microns-pore nuclear filters at the constant perfusion pressure 100 cm H2O. The plasma contained lymphocytes filtered by 80% better, than the lymphocyte-deprived plasma. The count and adhesive activity of platelets were similar in both plasmas.
Assuntos
Plaquetas/citologia , Heparina/farmacologia , Leucaférese , Linfócitos/citologia , Plasma/citologia , Plaquetoferese , Doadores de Sangue , Adesão Celular , Humanos , Contagem de Leucócitos , Filtros Microporos , Plasma/efeitos dos fármacos , Adesividade Plaquetária , Contagem de PlaquetasRESUMO
We investigated blood cells suspension filterability of 16 donors. The filtration was performed trough 5 microns-pore nuclear filters at constant perfusion pressure 10(5) din/cm2. We estimated also the adherence of leukocytes and platelets on nylon. The adherence of platelets and mononuclear leukocytes reduced the level of the suspension filterability only by 21% (p > 0.05). The presence of nonadhesive polynuclear leukocytes in the suspensions did not change practically their filterability. The addition in the suspensions of adhesive polynuclear leukocytes reduced suspension filterability dramatically.