Bottom-up processes drive isotopic variation in the South American sea lion Otaria flavescens across a 2300 km latitudinal gradient.

Spatial differences in the isotope values of widely distributed marine apex consumers may reflect geographical differences in the isotopic composition of basal resources (e.g., phytoplankton) fueling food webs (bottom-up effects) or spatial differences in the trophic ecology of the taxon of interest (top-down effects). We examined spatial variation in δ13C and δ15N values from 264 South American sea lions (SASL, Otaria flavescens) of different age classes (adults, subadults and juveniles), their putative prey consisting of pelagic and benthic coastal fishes, and particulate organic matter (POM) measured from locations situated across >2300 km of the Chilean coast (between 18°42' and 39°17' S). We used generalized least squares (GLS) models to compare the form of the relationship between δ13C and δ15N and latitude between the three functional groups. Our results show that SASL from northern, central, and southern areas were isotopically distinct, with individuals from the north having lower δ13C and higher δ15N values in comparison to individuals from the south. When the relationship for each functional group was modelled individually using GLS, results indicated that for each degree of increasing latitude δ15N decreased on average by 0.12‰ (POM), 0.15‰ (prey), and 0.14‰ (SASL), while δ13C increased by 0.06‰ (POM) and 0.05‰ in both prey and SASL. We suggest that the latitudinal differences observed in SASL δ13C and δ15N values reflect baseline isotopic variation rather than marked differences in trophic ecology of these widely distributed consumers.

Translation and cross-cultural adaptation of the "Quality of Life in Epilepsy (QOLIE-31-P)" Questionnaire for Chile.

PURPOSE: To report the translation and cross-cultural adaptation of the Quality of Life in Epilepsy (QOLIE-31-P) Questionnaire in patients with epilepsy (PWE) in Chile. METHODS: Translation from the original and posterior back-translation was performed by independent translators, two in each step. The final consensual translation was modified for the Chilean context and assessed by cognitive interviews with 12 PWE from Chile's public healthcare system, selected by quotas. RESULTS: Main changes made to the original questionnaire were the addition, in some items, of an alternative, indicating the nonexistence of limitations produced by epilepsy, examples to clarify some questions, and some minor wording modifications. There was no addition or deletion of items. CONCLUSION: A culturally adapted version of the QOLIE-31-P questionnaire was obtained in conditions to be assessed psychometrically in a sample of PWE in Chile.

Cell carbon content and biomass assessments of dinoflagellates and diatoms in the oceanic ecosystem of the Southern Gulf of Mexico.

This study assessed the cell carbon content and biomass for genera of dinoflagellates and diatoms in the oceanic ecosystem of the Southern Gulf of Mexico. Carbon content estimates were based on biovolume calculations derived from linear dimension measurements of individual cells and the approximate geometric body shape of each genus. Then, biomass assessments were performed for both groups in two gulf regions (Perdido and Coatzacoalcos) using these carbon content factors and cell abundances. After four seasonal cruises, 11,817 cells of dinoflagellates and 3,412 cells of diatoms were analyzed. Diverse body shapes and cell sizes were observed among 46 dinoflagellate genera and 37 diatom genera. Nano-cells of dinoflagellates (68% <20 µm) and micro-cells of diatoms (77% 20-200 µm, mostly 50-75 µm) were predominant. According to this cell-size structure, on average, diatoms contained 40% more carbon per cell than dinoflagellates. Contrasting carbon content estimates were observed within the genera of both microalgae. Large carbon averages (>10,000 pg C cell-1) were attributed to Gonyaulacal and some occasional genera of dinoflagellates (e.g., Pyrocystis and Noctiluca) and centric diatoms. In contrast, values up to 3 orders of magnitude lower were found for Peridinial and Gymnodinial dinoflagellates and pennate diatoms. Based on these carbon content estimates, which can be considered representative for most of this oceanic ecosystem, seasonal and regional differences were found in the biomass assessments conducted for these functional groups. Overall, dinoflagellates (mostly low-carbon Gymnodinales) had larger depth-integrated biomass than diatoms (mainly rich-carbon centric forms) within the euphotic zone. An exception to it was the late-summer cruise at the Coatzacoalcos region when a surface bloom of centric diatoms was observed in stations influenced by river runoff. This work contributes useful reference information for future ecological studies and models for understanding the biogeochemical functioning of this open-ocean ecosystem.

Dose simulations of an early 20th century kilovoltage pneumonia radiotherapy technique performed with a modern fluoroscope.

To simulate an early 20th century viral pneumonia radiotherapy treatment using modern fluoroscopy and evaluated it according to current dose guidelines. Monte Carlo was used to assess the dose distribution on an anthropomorphic phantom. Critical organs were: skin, breasts, esophagus, ribs, vertebrae, heart, thymus, and spinal cord. A 100 kVp beam with 3 mm Al HVL, 25 × 25 cm2 posterior-anterior (PA) field and 50 cm source-to-surface distance were simulated. Simulations had a resolution of 0.4 × 0.4 × 0.06 cm3 and a 6% uncertainty. Hundred percent dose was normalized to the skin surface and results were displayed in axial, coronal, and sagittal planes. Dose volume histograms were generated in MATLAB for further analysis. Prescription doses of 0.3, 0.5, and 1.0 Gy were applied to the 15% isodose for organ-dose comparison to current tolerances and potential risk of detriment. Ninety-five and ninety-seven percent of the right and left lung volumes, respectively, were well-covered by the 15% isodose line. For the 0.3, 0.5, and 1.0 Gy prescriptions, the maximum skin doses were 2.9, 4.8, and 9.6 Gy compared to a 2.0 Gy transient erythema dose threshold; left/right lung maximum doses were 1.44/1.46, 2.4/2.4, and 4.8/4.9 Gy compared to a 6.5 Gy pneumonitis and 30 Gy fibrosis thresholds; maximum heart doses were 0.5, 0.9, and 1.8 Gy compared to the 0.5 Gy ICRP-recommendation; maximum spinal cord doses were 1.4, 2.3, and 4.6 Gy compared to 7.0 Gy single fraction dose threshold. Maximum doses to other critical organs were below modern dose thresholds. A 100 kVp PA field could deliver a 0.3 Gy or 0.5 Gy dose without risk of complications. However, a 1.0 Gy dose treatment could be problematic. Critical organ doses could be further reduced if more than one treatment field is used.

Development, Spanish Normative Data, and Validation of a Social Cognition Battery in Prodromal Alzheimer's Disease and Multiple Sclerosis.

OBJECTIVES: The assessment of social cognition changes may be challenging, especially in the earliest stages of some neurodegenerative diseases. Our objective was to validate a social cognition battery from a multidomain perspective. In this regard, we aimed to adapt several tests, collect normative data, and validate them in prodromal Alzheimer's disease (AD) and multiple sclerosis (MS). METHODS: A total of 92 healthy controls, 25 prodromal AD, and 39 MS patients were enrolled. Age-, gender-, and education-matched control groups were created for comparisons. Social cognition battery was composed of an emotion-labeling task developed from FACES database, the Story-based Empathy test (SET), the Faux Pas test, and the Interpersonal Reactivity Index. Patients were also evaluated with a comprehensive cognitive battery to evaluate the other cognitive domains. Automatic linear modeling was used to predict each social cognition test's performance using the neuropsychological tests examining other cognitive domains. RESULTS: The reliability of the battery was moderate-high. Significant intergroup differences were found with medium-large effect sizes. Moderate correlations were found between social cognition battery and neuropsychological tests. The emotion labeling task and SET showed moderate correlations with age and education, and age, respectively. Regression-based norms were created considering the relevant demographic variables. Linear regression models including other neuropsychological tests explained between 7.7% and 68.8% of the variance of the social cognition tests performance. CONCLUSIONS: Our study provides a battery for the assessment of social cognition in prodromal AD and MS with Spanish normative data to improve the evaluation in clinical and research settings.

Activation of the Aryl Hydrocarbon Receptor (AHR) induces human glutathione S transferase alpha 1 (hGSTA1) expression.

Glutathione S-transferases (GSTs) are a key enzyme superfamily involved in the detoxification and cytoprotection of a wide variety of xenobiotics, such as carcinogens, anticancer drugs, environmental toxicants, and endogenously produced free radicals. In the liver, the hGSTA1 isoenzyme is the most abundant and catalyzes the glutathione conjugation of a wide range of electrophiles and has been the principal GST responsible for xenobiotic detoxification. Given the critical role of this enzyme in several cellular processes, particularly cell detoxification, understanding the molecular mechanisms underlying the regulation of hGSTA1 expression is critical. Therefore, the aim of the present study was to investigate whether AHR is involved in the modulation of hGSTA1 gene expression and to characterize the molecular mechanism through which AHR exerts this regulation. Two xenobiotic response elements (XREs) were located at -602 bp and -1030 bp from the transcription start site at the hGSTA1 gene promoter. After treatment of HepG2 cells with beta-naphthoflavone (ß-NF), an AHR agonist, induction of hGSTA1 mRNA was observed. This effect was mediated by the recruitment of AHR to the hGSTA1 gene promoter and its transactivation, as indicated by the ChIP, EMSA and luciferase activity assays. The increase in hGSTA1 transcription regulated by AHR also resulted in enhanced levels of hGSTA1 protein and activity. Taken together, our data suggest that AHR ligands have the potential to modify xenobiotic and endobiotic metabolism mediated by hGSTA1, thereby altering the detoxification of xenobiotics, steroidogenesis and the efficacy of chemotherapeutic agents.

Sample acidification has a predictable effect on isotopic ratios of particulate organic matter along the Chilean coast.

RATIONALE: Stable isotopes of carbon and nitrogen have proved to be valuable tools for researchers working across the different subfields of ecology. However, the chemical pretreatment of samples prior to analytical determination of stable isotope ratios can influence the results, and therefore conclusions regarding the ecology of the taxon or system under study. Here, we determined the effect of vapor acidification with concentrated HCl on the δ13 C and δ15 N values of particulate organic matter (POM), which are commonly used as baselines for studies of trophic ecology, or to understand oceanographic patterns. METHODS: Samples of marine POM were obtained along a large-scale latitudinal gradient (ca 3000 km) along the Chilean coast, along with a range of oceanographic variables thought to potentially influence inorganic carbon at each sampling location. A random subset of 50 samples was divided into two parts: one acidified by HCl fumigation treatment, and the other acting as a control. We compared paired differences in δ13 C and δ15 N values measured by continuous flow isotope ratio mass spectrometry and used a model selection approach to examine which oceanographic factor best explained shifts in values following acid treatment. RESULTS: Acidification resulted in statistically significant reductions in both δ13 C and δ15 N values, but the effect was relatively small. The model that best explained the differences between acidified and non-acidified δ13 C values included depth, salinity and sea-surface temperature at the sampling point. A regression of acidified on non-acidified δ13 C values shows that the treatment effect was strongest on samples more depleted in 13 C. CONCLUSIONS: The differences between δ13 C and δ15 N values in acidified and non-acidified samples are linear and predictable. This implies that the nature of the POM and its possible alteration during the acid treatment are important factors that support the reliable determination of the values of δ13 C and δ15 N of POM.

Fenilcetonuria de diagnóstico tardío y mutaciones asociadas en una familia ecuatoriana / Phenylketonuria of late diagnosis and associated mutations in an Ecuadorian family

La fenilcetonuria es un error innato del metabolismo, producido por mutaciones en el gen de la fenilalanina hidroxilasa. Se describe el caso de un adolescente de 15 años con diagnóstico tardío de fenilcetonuria, quien presenta retardo mental severo, convulsiones e hipopigmentación. En este estudio se realizó el diagnóstico molecular de fenilcetonuria y se detectó la mutación p.R252W en homocigosis en el gen que codifica para la fenilalanina hidroxilasa. La presencia de esta variante nos permitió inferir la falta de respuesta a la terapia con sapropterina, medicamento que actúa como cofactor de la enzima, por la ausencia de actividad enzimática residual reportada para esta variante. Debido al retraso psicomotor del paciente, se decidió aplicar terapia lúdica y fortalecimiento muscular a través de la intervención fisioterapéutica; sin embargo, no se observó una mejoría permanente al aplicar este tratamiento, motivado por la falta de continuidad.

Phenylketonuria is an inborn error of metabolism due to mutations on the phenylalanine hydroxylase gene. We described the case of a 15 years old-adolescent with late diagnosis of phenylketonuria, who presents severe mental retardation, convulsions and hypopigmentation. In this study, the molecular diagnosis of phenylketonuria was performed, detecting p.R252W mutation in homozygous state on the phenylalanine hydroxylase gene. The presence of this variant allowed us to infer the lack of response to drug therapy with sapropterina which works as an enzyme cofactor, due to the absence of residual enzymatic activity reported for the p.R252W variant. Physical therapy was applied through playful therapy and muscular strengthening, because of the psychomotor retardation present in the patient. The failing in continuing with the physical therapy program stopped the patient´s improvement.

Dexamethasone induces human glutathione S transferase alpha 1 (hGSTA1) expression through the activation of glucocorticoid receptor (hGR).

The glutathione S transferases (GSTs) are a superfamily of isoenzymes that play an important role in xenobiotic and endobiotic detoxification, cellular protection from oxidative stress and modulation of signalling pathways. Human GSTA1 (hGSTA1), a cytosolic isoform, is the most abundant GST in the liver and is involved in the metabolism of carcinogenic compounds, chemotherapeutic agents and lipid peroxidation products. However, the molecular mechanism underlying the regulation of hGSTA1 expression is not well understood. Therefore, the aim of the present study was to better understand the regulation of hGSTA1 gene expression. Putative response elements for several nuclear receptors, including the glucocorticoid receptor (GR), have been identified at the hGSTA1 gene promoter located at -896bp, -863bp and -727bp from the transcription start site. After dexamethasone (DEX) treatment, the GR induces hGSTA1 expression at the transcriptional level. The characterisation of this effect reveals that the GR binds to several glucocorticoid response elements (GREs) located at the hGSTA1 gene promoter. This interaction also results in the transactivation of the hGSTA1 gene promoter together with an increase of the hGSTA1 mRNA levels as well as the protein and activity levels in HepG2 cells. Together, the present results suggest that glucocorticoids have the potential to alter the xenobiotic and endobiotic metabolism mediated by hGSTA1.

Efectos de un programa de danzaterapia en la composición corporal y calidad de vida de mujeres mayores españolas con sobrepeso.

Objetivo: este estudio tuvo como objetivo probar la eficacia de un programa de danzaterapia en la composición corporal y calidad de vida en mujeres mayores de 65 años, que no realizan ejercicio físico de forma habitual. Métodos: cincuenta y dos mujeres sedentarias (edad media de 69,27 ± 3,85 años) fueron asignadas al azar para realizar un programa de danzaterapia (n = 27) o formar parte del grupo control (n = 25). El grupo de danza, participó durante ocho semanas en un programa de danza, tres sesiones semanales de 50 minutos cada una, basado en danza popular española (sevillanas) y ballet. Ambos grupos reciben información sobre la importancia de la alimentación y del ejercicio físico al comienzo y mitad del estudio. La circunferencia de la cintura, la presión arterial, el índice de masa corporal y la calidad de vida se evaluaron al inicio y después del tratamiento en ambos grupos. El análisis estadístico se realizó con un 2 × 2 ANOVA.Resultados: las mujeres del grupo de danza mostraron reducción significativa de la circunferencia de la cintura (p = 0,001) en comparación con las del grupo control. Los cambios en el IMC y la calidad de vida, aunque mejoraron en el grupo de danza, no alcanzaron significación estadística. Conclusión: la danzaterapia española es una forma de ejercicio físico eficaz para reducir la grasa visceral y prevenir comorbilidades en mujeres mayores, contribuyendo a cambiar estilos de vida poco saludables.

Efectos de un programa de danzaterapia en la composición corporal y calidad de vida de mujeres mayores españolas con sobrepeso / Effects of a program of dance therapy on body composition and quality of life of Spanish older overweight women

Objetivo: este estudio tuvo como objetivo probar la eficacia de un programa de danzaterapia en la composición corporal y calidad de vida en mujeres mayores de 65 años, que no realizan ejercicio físico de forma habitual. Métodos: cincuenta y dos mujeres sedentarias (edad media de 69,27 ± 3,85 años) fueron asignadas al azar para realizar un programa de danzaterapia (n = 27) o formar parte del grupo control (n = 25). El grupo de danza, participó durante ocho semanas en un programa de danza, tres sesiones semanales de 50 minutos cada una, basado en danza popular española (sevillanas) y ballet. Ambos grupos reciben información sobre la importancia de la alimentación y del ejercicio físico al comienzo y mitad del estudio. La circunferencia de la cintura, la presión arterial, el índice de masa corporal y la calidad de vida se evaluaron al inicio y después del tratamiento en ambos grupos. El análisis estadístico se realizó con un 2 × 2 ANOVA. Resultados: las mujeres del grupo de danza mostraron reducción significativa de la circunferencia de la cintura (p = 0,001) en comparación con las del grupo control. Los cambios en el IMC y la calidad de vida, aunque mejoraron en el grupo de danza, no alcanzaron significación estadística. Conclusión: la danzaterapia española es una forma de ejercicio físico eficaz para reducir la grasa visceral y prevenir comorbilidades en mujeres mayores, contribuyendo a cambiar estilos de vida poco saludables (AU)

Objective: This study aimed to test the effectiveness of a dance therapy program on body composition and quality of life in women over 65, who do not perform physical exercise regularly. Methods: Fifty-two sedentary older women (mean age 69.27 ± 3.85 years) were randomly assigned to receive either dance therapy (n = 27) or part of the control group (n = 25). The intervention group participated in eight weeks of dance therapy, three sessions weekly/50 min, based on Spanish folk dance and ballet. Both groups receive information about the importance of nutrition and physical activity at the beginning and middle of the study. The waist circumference, blood pressure, body mass index and quality of life were assessed at baseline and after treatment in both groups. Statistical analysis was performed using a 2 × 2 ANOVA. Results: Women in the intervention group showed significant reduction in waist circumference (p = 0,001) compared to those in the control group. Changes in BMI and quality of life although they improved in the dance group did not reach statistical significance. Conclusion: The Spanish dance therapy is an effective form of exercise to reduce visceral fat and prevent comorbidities in older women, helping to change unhealthy life styles (AU)

Efectos de un programa de terapia de baile en la calidad de vida, el sueño y la presión arterial en mujeres de mediana edad: un ensayo controlado aleatorizado / Effects of a dance therapy programme on quality of life, sleep and blood pressure in middle-aged women: A randomised controlled trial

Fundamento y objetivo: Existe evidencia sobre los efectos positivos de la danza, como la mejora de los parámetros cardiovasculares y del sueño. El objetivo del presente estudio ha sido explorar si un programa de terapia de baile es capaz de mejorar el sueño y la presión arterial en mujeres de mediana edad, prehipertensas e hipertensas. Métodos: Se diseñó un ensayo aleatorizado y controlado, donde las participantes fueron asignadas a uno de 2 grupos: grupo control (donde las participantes continuaron con sus actividades y medicación habitual) o grupo de terapia de baile (donde las participantes siguieron un programa de terapia de baile, además de su medicación). La intervención consistió en un programa de baile-entrenamiento específico y progresivo durante 8 semanas, a razón de 3 sesiones semanales. Los pasos de baile fueron específicamente seleccionados para mejorar el equilibrio, desplazando el cuerpo y reubicando el centro de gravedad. Las medidas de resultado fueron la presión arterial, la calidad del sueño y la calidad de vida, estas 2 últimas medidas por elPittsburgh Sleep Quality Index y el Cuestionario Europeo sobre Calidad de Vida, respectivamente. Resultados: Se asignó al azar, a un total de 67 mujeres de mediana edad prehipertensas e hipertensas a un grupo de intervención (n=35), o como controles (n=32), después de las pruebas basales. El grupo de intervención informó de mejoras significativas en los valores de la presión arterial (p<0,01), así como en la calidad del sueño (p<0,05) y la calidad de vida (p<0,001) en comparación con el grupo control. Conclusión: El programa de terapia de baile mejoró la presión arterial, el sueño y la calidad de vida en mujeres de mediana edad prehipertensas e hipertensas, proporcionando la base para continuar la investigación con estudios más amplios (AU)

Background and objective: Evidence suggests that dance therapy may have positive effects in areas such as cardiovascular parameters and sleep. The aim of the present study is to explore whether a dance therapy programme improves sleep and blood pressure in a population of middle-aged pre-hypertensive and hypertensive women. Methods: A randomised controlled trial was conducted, in which participants were assigned to one of 2 groups: standard care (with usual activities and medication) or dance therapy (in which the participants followed a dance therapy programme, in addition to their medication). The intervention was an 8-week, 3-times-per-week, progressive and specific group dance-training programme. The dance steps were specifically designed to improve balance by shifting the body and relocating the centre of gravity. The main measures obtained were blood pressure, sleep quality and quality of life, measured by the Pittsburgh Sleep Quality Index and the European Quality of Life Questionnaire. Results: Sixty-seven pre-hypertensive and hypertensive middle-aged women were randomised to either an intervention group (n=35) or a control group (n=32) after baseline testing. The intervention group reported a significant improvement in blood pressure values (P<.01), as well as in sleep quality (P<.05) and quality of life (P<.001), compared to the control group. Conclusion: The dance therapy programme improved blood pressure, sleep and quality of life in pre-hypertensive and hypertensive middle-aged women, and constitutes an interesting basis for larger-scale research (AU)

[Effects of a dance therapy programme on quality of life, sleep and blood pressure in middle-aged women: A randomised controlled trial]. / Efectos de un programa de terapia de baile en la calidad de vida, el sueño y la presión arterial en mujeres de mediana edad: un ensayo controlado aleatorizado.

BACKGROUND AND OBJECTIVE: Evidence suggests that dance therapy may have positive effects in areas such as cardiovascular parameters and sleep. The aim of the present study is to explore whether a dance therapy programme improves sleep and blood pressure in a population of middle-aged pre-hypertensive and hypertensive women. METHODS: A randomised controlled trial was conducted, in which participants were assigned to one of 2 groups: standard care (with usual activities and medication) or dance therapy (in which the participants followed a dance therapy programme, in addition to their medication). The intervention was an 8-week, 3-times-per-week, progressive and specific group dance-training programme. The dance steps were specifically designed to improve balance by shifting the body and relocating the centre of gravity. The main measures obtained were blood pressure, sleep quality and quality of life, measured by the Pittsburgh Sleep Quality Index and the European Quality of Life Questionnaire. RESULTS: Sixty-seven pre-hypertensive and hypertensive middle-aged women were randomised to either an intervention group (n=35) or a control group (n=32) after baseline testing. The intervention group reported a significant improvement in blood pressure values (P<.01), as well as in sleep quality (P<.05) and quality of life (P<.001), compared to the control group. CONCLUSION: The dance therapy programme improved blood pressure, sleep and quality of life in pre-hypertensive and hypertensive middle-aged women, and constitutes an interesting basis for larger-scale research.

Cooperatively assembling donor-acceptor superstructures direct energy into an emergent charge separated state.

A novel supramolecular system composed of diketopyrrolopyrrole electron donors and perylene derived bisimide (PDI) electron acceptors forms superstructures that undergo fast photoinduced charge separation following assembly. This bioinspired route toward functional hierarchical structures, whereby assembly and electronic properties are closely coupled, could lead to new materials for artificial photosynthesis and organic electronics.

Electrochemical determination of heme-linked pKa values and the importance of using fluoride binding in heme proteins.

The ultraviolet-visible (UV-vis) spectroelectrochemical measurements of heme proteins in the presence of a heme-bound fluoride ion can be used as a probe for heme-linked ionizations of acid-base groups in the heme pocket. A detailed study of the pH dependence of the midpoint potential of skeletal horse myoglobin (Mb) with a heme-bound fluoride ion (Mb-F) reveals how protonation of the distal histidine (H64) changes the redox properties of the protein with a determined pKa of 5.3. In addition, fluoride binding in myoglobin provides a stabilization of -1.9 kcal/mol of the ferric Mb-F relative to ferric Mb without fluoride.

Grado de transportación del canal radicular de tres sistemas de instrumentación rotatoria: estudio a través de tomografía computarizada Cone beam / Degree of root canal transportation of three rotary instrumentation systems: a cone-beam computed tomography study

Introducción: en la preparación del sistema de canales radiculares se debe lograr una conformación progresivamente cónica desde apical hacia coronal, sin generar accidentes de procedimiento. Se define como transportación al cambio de ubicación espacial del canal radicular con respecto a su ubicación original, produciéndose así el desgaste no proporcional de alguna de las paredes dentinarias en relación con la anatomía original de este. Durante la última década, se han fabricado nuevos instrumentos endodónticos rotatorioscon base en níquel-titanio (NiTi), lo que provee mayor flexibilidad y resistencia a la fractura, disminuye el tiempo de trabajo y la fatiga del operador, facilitando así la conformación adecuada del canal y reduciendo accidentes durante los procedimientos.Método: estudio in vitro, de 45 canales radiculares humanos superiores e inferiores con curvaturas severas seleccionados a través de radiografías convencionales. Se formaron 3 grupos de 15 conductos que fueron preparados con los sistemas de instrumentación rotatoria antes mencionados. Se utilizótecnología Cone beam para registro de imágenes previas y posteriores a la instrumentación, las que se tomaron a través de rodetes de siliconaconformando una arcada dentaria con la idea de ser un montaje reproducible posinstrumentación. Se hicieron mediciones en los cortes tomados a los 2, 4, 6, y 8 mm desde el ápice por canal instrumentado, en cada corte se hicieron 4 mediciones, pared vestibular, lingual, furca y cara libre. Los datos obtenidos fueron analizados mediante test Wilcoxon para muestras pareadas y el test ANOVA. Resultados yconclusiones: los resultados indican que el sistema ProTaper Universal (Dentsplay-Maillefer, Switzerland) produce mayor transportación delcanal radicular a nivel del tercio medio en comparación con los sistemas RaCe (FKG-Dentaire, Switzerland) y K3 (SybrondEndo, USA).

Introduction: the preparation of root canals should provide a progressively tapered shape from apical towards coronal, without producing procedural errors. Transportation is defined as the change in location of the root canal with respect to its original position, producing unbalanced wear in any of the dentine walls in relation to their original anatomy. New rotary endodontic instruments have been developed during the latest decade with nickel-titanium (NiTi), a material that provides increased flexibility and fracture strength, reduces working time and operator fatigue, and facilitates proper canal preparation while reducing procedural errors. Method: this wasan in vitro study on 45 upper and lower human root canals with moderate to severe curvatures, selected through conventional radiograph They were sorted out in 3 groups of 15 canals that were prepared with the aforementioned rotary instrumentation systems. The cone beam technology was used to record images before and after instrumentation; these images were obtained by means of a silicon rim that formed a dental arch intended to serve as a post-instrumentation reproducible assembly. Measurements were made in sections taken at 2, 4, 6, and 8 mm from the apex via instrumented canal; four measurements were made in each section: the buccal, lingual, furcation and free side walls. The obtained data were analyzed with the Wilcoxon test for paired samples and the ANOVA test.Results and conclusions: the results suggest that the ProTaper Universal system (Dentsply-Maillefer, Switzerland) produces the most root canal transportation at the middle third compared with the RaCe (FKG-Dentaire, Switzerland) and K3 (SybronEndo, USA) systems. Also, the K3 system showed more conservative wear at 2, 4 and 6 mm of root canal instrumentation, which makes it suitable for instrumenting thin and curved canals.

Prevalencia y necesidad de tratamiento de trastornos temporomandibulares en una población Chilena / Prevalence and need for treatment of temporomandibular disorders in a Chilean population

Fundamento: los criterios diagnósticos para la investigación de trastornos temporomandibulares se han reconocido internacionalmente por su utilidad clínica y aplicación en investigación epidemiológica. Objetivo: determinar la prevalencia de trastornos temporomandibulares y las características asociadas a esta patología, además se evaluó la necesidad de tratamiento real en pacientes con dolor o limitación funcional de acuerdo a los criterios diagnósticos de investigación de los trastornos temporomandibulares. Método: la muestra estuvo compuesta por 194 mujeres y 75 hombres que consultaron para tratamiento odontológico en dos hospitales estatales de Chile, con una edad promedio de 51 años. Todos los pacientes diligenciaron la versión validada de la encuesta y de la ficha clínica. Resultados: el 53,51 % de los sujetos presentó al menos un trastorno temporomandibular. Las mujeres presentaron una mayor prevalencia de trastornos temporomandibulares (119 individuos; 61,3 %) comparada con los hombres (25 pacientes; 33,3 %). Se encontraron 101 pacientes con trastornos temporomandibulares de tipo muscular. Igualmente se hallaron 67 sujetos con trastornos temporomandibulares de tipo articular. Un total de 179 individuos (66,5 %) presentaron niveles de depresión moderados y severos. Por otra parte, el 41,2 % de los pacientes mostraron al menos un diagnostico que requiere tratamiento odontológico. Conclusiones: en el presente estudio se observó una prevalencia elevada de trastornos temporomandibulares que se presentan con mayor frecuencia en mujeres. Igualmente, los trastornos de tipo muscular son más asiduos que los de tipo articular. Es importante destacar que casi la mitad de la población estudiada presentó un diagnóstico que requiere tratamiento.

Background: diagnostic criteria for temporomandibular disorders research have been recognized internationally for its clinical utility and application in epidemiological research. Objective: to determine the prevalence of temporomandibular disorders and characteristics associated with this pathology, also it was evaluated the need for real treatment in patients with pain or functional limitation according to research diagnostic criteria for temporomandibular disorders. Methods: the sample consisted of 194 women and 75 men with dental treatment in two state hospitals in Chile, with a mean age of 51 years. All patients were required to fill the validated version of the survey and the clinical record. Results:The 53,51 % of patients had at least one temporomandibular disorders. Women showed a higher prevalence of temporomandibular disorders (119 individuals, 61,3 %) compared to men (25 patients, 33,3 %). One hundred and one patients with temporomandibular disorders of muscular type were found and 67 with temporomandibular joint disorders. A total of 179 individuals (66,5 %) showed moderate and severe levels of depression. On the other hand, 41,2 % of patients showed at least one diagnosis that required dental treatment. Conclusions: in the present study a high prevalence of temporomandibular disorders was observed, with more frequency in women. Similarly, muscle-type disorders are more frequent than joint type disorders. It's very important to note that almost half of the studied population presented a diagnosis that requires treatment.

Evaluación tridimensional de la vía aérea mediante tomografía computarizada de haz de cono / Three-dimensional assessment of airway through cone beam computed tomography

Fundamento: los cambios en la función normal de la vía aérea durante el proceso activo de crecimiento facial pueden tener una influencia significativa en el desarrollo facial. Objetivo: presentar la utilidad de la evaluación de la vía aérea mediante tomografía computarizada de haz de cono. Método: se revisaron las imágenes de 250 mujeres y 128 hombres a través del programa Sidexis para Galileos. La selección de las zonas a medir se determinó mediante las coordenadas que pasan por el conducto nasopalatino en el plano sagital y por la zona interproximal de los incisivos centrales superiores, en el plano coronal. Resultados: se observó una correlación negativa moderada pero estadísticamente significativa entre la edad del paciente y el área de la vía aérea en ambos sexos. Se encontró una correlación positiva moderada, estadísticamente significativa entre la longitud del velo del paladar y la edad en los dos sexos. Se presentó también una correlación del área de sección transversal y la longitud del paladar blando. Conclusiones: la Tomografía Computarizada de Haz de Cono proporciona una imagen tridimensional confiable de la relación espacial entre los dientes y la cara y sus estructuras anatómicas circundantes permitiendo una evaluación adecuada de los tejidos.

Background: changes in the normal function of the airway during the active process of facial growth can have a significant influence on facial development. Objective: to present the usefulness of the evaluation of airway Beam Computed Tomography Cone. Methods: images of 250 women and 128 men using the program Sidexis to Galilee were reviewed. The selection of areas to be measured was determined by the coordinates that pass through the canal in the sagittal plane nasopalatine and interproximal area of the upper central incisors in the coronal plane. Results:a moderate negative correlation, but statistically significant between patient age and area of the airway in both sexes was found. There was a moderate positive correlation, statistically significant between the length of the soft palate and age in both sexes. A correlation of cross-sectional area and length of the soft palate was also showed. Conclusions: the Beam Computed Tomography Cone provides a reliable three-dimensional image of the spatial relationship between the teeth and face and surrounding anatomical structures allowing adequate assessment of the tissues.