In Vitro and In Vivo Cysticidal Effects of Carica Papaya Cell Suspensions.

Parasitic diseases are a major public health problem worldwide. Plant-derived products appear to be ideal candidates from a biotechnological perspective, being sustainable and environmentally friendly. The antiparasitic properties of Carica papaya have been attributed to some of its components, including papain and other compounds that are concentrated in the latex and seeds. This study demonstrated in vitro a high and insignificantly different cysticidal activity of soluble extract that was obtained after the disruption of nontransformed wild-type (WT) cells as well as transformed papaya calluses (PC-9, PC-12, and PC-23) and papaya cell suspensions (CS-9, CS-12, and CS-23). In vivo, cell suspensions of CS-WT and CS-23 that had been previously lyophilized were tested with respect to their cysticidal effects, compared with those of three commercial antiparasitic drugs. CS-WT and CS-23 together reduced the number of cysticerci, the number of buds, and the percentage of calcified cysticerci in a similar extent to albendazole and niclosamide, whereas ivermectin was less effective. Mice were then orally immunized with CS-23 that expressed the anti-cysticercal KETc7 antigen (10 µg/mouse), CS-WT (10 mg/mouse), or both together to evaluate their preventive properties. CS-23 and CS-WT significantly reduced the expected parasite and increased the percentage of calcified cysticerci as well as recovery, being more effective when employed together. The results reported in this study support the feasibility of the development of an anti-cysticercosis vaccine from cells of C. papaya in in vitro cultures, as they are a source of an anthelmintic, natural, and reproducible product.

Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus.

Parasitic diseases have a major impact on human and animal health worldwide. Despite the availability of effective anti-parasitic drugs, their excessive and uncontrolled use has promoted the emergence of drug resistance, severely affecting ecosystems and human health. Thus, developing environmentally friendly antiparasitic treatments is urgently needed. Carica papaya has shown promising effects against infectious diseases. C. papaya embryogenic calluses were genetically modified by our research team to insert immunogenic peptides with the goal of developing an oral anti-cysticercosis vaccine. Among these callus cell lines, one labeled as CF-23, which expresses the KETc7 immunogenic peptide, induced the highest protection levels against experimental cysticercosis. In the process of designing a natural antiparasitic product based on C. papaya that simultaneously induced immunity against cysticercosis, both transformed (SF-23) and untransformed (SF-WT) suspension cultures were produced and optimized. Our results showed a better duplication time (td) for SF-23 (6.9 days) than SF-WT (13.02 days); thus, the SF-23 line was selected for scale-up in a 2-L airlift bioreactor, reaching a td of 4.4 days. This is the first time that a transgenic line of C. papaya has been grown in an airlift bioreactor, highlighting its potential for scale-up cultivation in this type of reactor. Considering the previously reported nematocidal activity of C. papaya tissues, their activity against the nematode Haemonchus contortus of aqueous extracts of SF-WT and SF-23 was explored in this study, with promising results. The information herein reported will allow us to continue the cultivation of the transgenic cell suspension line of C. papaya under reproducible conditions, to develop a new anti-parasitic product.

Autopercepción de la calidad de vida del adulto mayor con diagnóstico de cáncer sometido a quimioterapia ambulatoria. HNERM. 2013 / Autoperception of the quality of life of the elderly diagnosed with cancer undergoing outpatient chemotherapy. HNERM. 2013

El estudio titulado: "Autopercepción de la calidad de vida del adulto mayor con diagnóstico de Cáncer sometido a quimioterapia ambulatoria. HNERM. 2013". Tuvo como objetivo general: Determinar la autopercepción de la calidad de vida del adulto mayor con diagnóstico de Cáncer sometido a quimioterapia ambulatoria. Material y Método: El estudio fue de nivel aplicativo, tipo cuantitativo, método descriptivo de corte transversal. Población y Muestra: El muestreo fue no probabilístico a conveniencia del investigador y estuvo conformado por 44 pacientes adultos mayores que asistieron a la Unidad de Quimioterapia durante el mes de mayo. Técnica e Instrumento: La técnica fue la encuesta y el instrumento la escala tipo Likert modificada con 38 enunciados presentados en base a las preguntas del cuestionario WHOQOL-Bref de la OMS y el cuestionario multidimensional QLQ-C30. Resultados: Del 100 por ciento (44) de pacientes adultos mayores, el 25 por ciento (11) tienen una Autopercepción desfavorable de su calidad de vida; el 43.2 por ciento (19) tienen una Autopercepción medianamente favorable y 31.8 por ciento (14) tienen una Autopercepción favorable de su calidad de vida. Conclusiones: El mayor porcentaje de adultos mayores encuestados tiene una Autopercepción medianamente favorable de su calidad de vida seguida por un porcentaje significativo que tiene una Autopercepción favorable.

The qualified study: “Autoperception of the quality of life of the major adult with diagnosis of Cancer submitted to ambulatory chemotherapy. HNERM. 2013". It had as general aim: To determine the Autoperception of the quality of life of the major adult with diagnosis of Cancer submitted to ambulatory chemotherapy. The study was of applicative level, quantitative type, and descriptive method of transverse court. The sampling went not probabilistic to convenience of the investigator and was shaped by 44 adult major patients who were present at the Unit of Chemotherapy during May. The technique was the survey and the instrument the scale type Likert modified with 38 terms of reference adapted on the basis of the questions of the questionnaire WHOQOL-Bref of the WHO and the multidimensional questionnaire QLQ-C30. Results: Of 100 per cent (44) of adult major patients, 25 per cent (11) they have an unfavorable Autoperception of his quality of life; 43.2 per cent (19) has a moderately favorable Autoperception and 31.8 per cent (14) has a favorable Autoperception of his quality of life. Conclusions: The highest percentage of major polled adults has a moderately favorable Autoperception of his quality of life followed by a significant percentage that has a favorable Autoperception.