Water Deficit, Nitrogen Availability, and Their Combination Differently Affect Floral Scent Emission in Three Brassicaceae Species.

Floral scent plays a central role in plant-pollinator interactions, as flower visitors can discriminate between scent differences to recognize and forage on rewarding flowers. Changes in scent compositions might therefore lead to recognition mismatches between host plants and flower visitors. An understanding of the phenotypic plasticity of floral scent, especially in crop species, is becoming important because of climate change, e.g., increasing drought periods, and other anthropogenic influences, e.g., nitrogen (N) deposition. We have investigated the effects of the combination of progressive water deficits (dry-down) and N supplementation on floral scent emission in three Brassicaceae species (cultivated vs. wild). Individuals were randomly assigned to one of four treatments: (1) well-watered without N supplementation; (2) well-watered with N supplementation; (3) dry-down without N supplementation; (4) dry-down with N supplementation. We collected scent on day 0, 2, 7, and 14 after the commencement of the watering treatment. All samples were analyzed using gas chromatography coupled with mass spectrometry. We found that the highly cultivated Brassica napus had the lowest overall emission rate; its scent composition was affected by the interaction of watering treatment and N supplementation. Scent bouquets of the cultivated Sinapis alba also changed under these treatments. Scent bouquets of the common weed Sinapis arvensis were affected by watering treatment, but not by time and N supplementation. Furthermore, the influence of treatments on the emission rate of single compounds was highly compound-specific. Nonetheless, our study revealed that especially terpenes were negatively affected by drought-stress.

Bumblebee Behavior on Flowers, but Not Initial Attraction, Is Altered by Short-Term Drought Stress.

Climate change is leading to increasing drought and higher temperatures, both of which reduce soil water levels and consequently water availability for plants. This reduction often induces physiological stress in plants, which in turn can affect floral development and production inducing phenotypic alterations in flowers. Because flower visitors notice and respond to small differences in floral phenotypes, changes in trait expression can alter trait-mediated flower visitor behavior. Temperature is also known to affect floral scent emission and foraging behavior and, therefore, might modulate trait-mediated flower visitor behavior. However, the link between changes in flower visitor behavior and floral traits in the context of increasing drought and temperature is still not fully understood. In a wind-tunnel experiment, we tested the behavior of 66 Bombus terrestris individuals in response to watered and drought-stressed Sinapis arvensis plants and determined whether these responses were modulated by air temperature. Further, we explored whether floral traits and drought treatment were correlated with bumblebee behavior. The initial attractiveness of drought and watered plants did not differ, as the time to first visit was similar. However, bumblebees visited watered plants more often, their visitation rate to flowers was higher on watered plants, and bumblebees stayed for longer, indicating that watered plants were more attractive for foraging. Bumblebee behavior differed between floral trait expressions, mostly independently of treatment, with larger inflorescences and flowers leading to a decrease in the time until the first flower visit and an increase in the number of visits and the flower visitation rate. Temperature modulated bumblebee activity, which was highest at 25°C; the interaction of drought/water treatment and temperature led to higher visitation rate on watered plants at 20°C, possibly as a result of higher nectar production. Thus, bumblebee behavior is influenced by the watered status of plants, and bumblebees can recognize differences in intraspecific phenotypes involving morphological traits and scent emission, despite overall morphological traits and scent emission not being clearly separated between treatments. Our results indicate that plants are able to buffer floral trait expressions against short-term drought events, potentially to maintain pollinator attraction.