Tumor associated macrophages as key contributors and targets in current and future therapies for melanoma.

INTRODUCTION: Despite the success of immunotherapies for melanoma in recent years, there remains a significant proportion of patients who do not yet derive benefit from available treatments. Immunotherapies currently licensed for clinical use target the adaptive immune system, focussing on Tcell interactions and functions. However, the most prevalent immune cells within the tumor microenvironment (TME) of melanoma are macrophages, a diverse immune cell subset displaying high plasticity, to which no current therapies are yet directly targeted. Macrophages have been shown not only to activate the adaptive immune response, and enhance cancer cell killing, but, when influenced by factors within the TME of melanoma, these cells also promote melanoma tumorigenesis and metastasis. AREAS COVERED: We present a review of the most up-to-date literatureavailable on PubMed, focussing on studies from within the last 10 years. We also include data from ongoing and recent clinical trials targeting macrophages in melanoma listed on clinicaltrials.gov. EXPERT OPINION: Understanding the multifaceted role of macrophages in melanoma, including their interactions with immune and cancer cells, the influence of current therapies on macrophage phenotype and functions and how macrophages could be targeted with novel treatment approaches, are all critical for improving outcomes for patients with melanoma.

From Software to Hardware: A Case Series of Functional Neurological Symptoms and Cerebrovascular Disease.

OBJECTIVE: Neuroimaging studies have identified alterations in both brain structure and functional connectivity in patients with functional neurological disorder (FND). For many patients, FND emerges from physical precipitating events. Nevertheless, there are a limited number of case series in the literature that describe the clinical presentation and neuroimaging correlates of FND following cerebrovascular disease. METHODS: The authors collected data from two clinics in the United Kingdom on 14 cases of acute, improving, or delayed functional neurological symptoms following cerebrovascular events. RESULTS: Most patients had functional neurological symptoms that were localized to cerebrovascular lesions, and the lesions mapped onto regions known to be part of functional networks disrupted in FND, including the thalamus, anterior cingulate gyrus, insula, and temporoparietal junction. CONCLUSIONS: The findings demonstrate that structural lesions can lead to FND symptoms, possibly explained through changes in relevant mechanistic functional networks.

Toward a Vision-Based Intelligent System: A Stacked Encoded Deep Learning Framework for Sign Language Recognition.

Sign language recognition, an essential interface between the hearing and deaf-mute communities, faces challenges with high false positive rates and computational costs, even with the use of advanced deep learning techniques. Our proposed solution is a stacked encoded model, combining artificial intelligence (AI) with the Internet of Things (IoT), which refines feature extraction and classification to overcome these challenges. We leverage a lightweight backbone model for preliminary feature extraction and use stacked autoencoders to further refine these features. Our approach harnesses the scalability of big data, showing notable improvement in accuracy, precision, recall, F1-score, and complexity analysis. Our model's effectiveness is demonstrated through testing on the ArSL2018 benchmark dataset, showcasing superior performance compared to state-of-the-art approaches. Additional validation through an ablation study with pre-trained convolutional neural network (CNN) models affirms our model's efficacy across all evaluation metrics. Our work paves the way for the sustainable development of high-performing, IoT-based sign-language-recognition applications.

An IoT Enable Anomaly Detection System for Smart City Surveillance.

Since the advent of visual sensors, smart cities have generated massive surveillance video data, which can be intelligently inspected to detect anomalies. Computer vision-based automated anomaly detection techniques replace human intervention to secure video surveillance applications in place from traditional video surveillance systems that rely on human involvement for anomaly detection, which is tedious and inaccurate. Due to the diverse nature of anomalous events and their complexity, it is however, very challenging to detect them automatically in a real-world scenario. By using Artificial Intelligence of Things (AIoT), this research work presents an efficient and robust framework for detecting anomalies in surveillance large video data. A hybrid model integrating 2D-CNN and ESN are proposed in this research study for smart surveillance, which is an important application of AIoT. The CNN is used as feature extractor from input videos which are then inputted to autoencoder for feature refinement followed by ESN for sequence learning and anomalous events detection. The proposed model is lightweight and implemented over edge devices to ensure their capability and applicability over AIoT environments in a smart city. The proposed model significantly enhanced performance using challenging surveillance datasets compared to other methods.

Solar Power Prediction Using Dual Stream CNN-LSTM Architecture.

The integration of solar energy with a power system brings great economic and environmental benefits. However, the high penetration of solar power is challenging due to the operation and planning of the existing power system owing to the intermittence and randomicity of solar power generation. Achieving accurate predictions for power generation is important to provide high-quality electric energy for end-users. Therefore, in this paper, we introduce a deep learning-based dual-stream convolutional neural network (CNN) and long short-term nemory (LSTM) network followed by a self-attention mechanism network (DSCLANet). Here, CNN is used to learn spatial patterns and LSTM is incorporated for temporal feature extraction. The output spatial and temporal feature vectors are then fused, followed by a self-attention mechanism to select optimal features for further processing. Finally, fully connected layers are incorporated for short-term solar power prediction. The performance of DSCLANet is evaluated on DKASC Alice Spring solar datasets, and it reduces the error rate up to 0.0136 MSE, 0.0304 MAE, and 0.0458 RMSE compared to recent state-of-the-art methods.

Deep Multi-Scale Features Fusion for Effective Violence Detection and Control Charts Visualization.

The study of automated video surveillance systems study using computer vision techniques is a hot research topic and has been deployed in many real-world CCTV environments. The main focus of the current systems is higher accuracy, while the assistance of surveillance experts in effective data analysis and instant decision making using efficient computer vision algorithms need researchers' attentions. In this research, to the best of our knowledge, we are the first to introduce a process control technique: control charts for surveillance video data analysis. The control charts concept is merged with a novel deep learning-based violence detection framework. Different from the existing methods, the proposed technique considers the importance of spatial information, as well as temporal representations of the input video data, to detect human violence. The spatial information are fused with the temporal dimension of the deep learning model using a multi-scale strategy to ensure that the temporal information are properly assisted by the spatial representations at multi-levels. The proposed frameworks' results are kept in the history-maintaining module of the control charts to validate the level of risks involved in the live input surveillance video. The detailed experimental results over the existing datasets and the real-world video data demonstrate that the proposed approach is a prominent solution towards automated surveillance with the pre- and post-analyses of violent events.

An Efficient Pest Detection Framework with a Medium-Scale Benchmark to Increase the Agricultural Productivity.

Insect pests and crop diseases are considered the major problems for agricultural production, due to the severity and extent of their occurrence causing significant crop losses. To increase agricultural production, it is significant to protect the crop from harmful pests which is possible via soft computing techniques. The soft computing techniques are based on traditional machine and deep learning-based approaches. However, in the traditional methods, the selection of manual feature extraction mechanisms is ineffective, inefficient, and time-consuming, while deep learning techniques are computationally expensive and require a large amount of training data. In this paper, we propose an efficient pest detection method that accurately localized the pests and classify them according to their desired class label. In the proposed work, we modify the YOLOv5s model in several ways such as extending the cross stage partial network (CSP) module, improving the select kernel (SK) in the attention module, and modifying the multiscale feature extraction mechanism, which plays a significant role in the detection and classification of small and large sizes of pest in an image. To validate the model performance, we develop a medium-scale pest detection dataset that includes the five most harmful pests for agriculture products that are ants, grasshopper, palm weevils, shield bugs, and wasps. To check the model's effectiveness, we compare the results of the proposed model with several variations of the YOLOv5 model, where the proposed model achieved the best results in the experiments. Thus, the proposed model has the potential to be applied in real-world applications and further motivate research on pest detection to increase agriculture production.

Improving the Efficiency of Multistep Short-Term Electricity Load Forecasting via R-CNN with ML-LSTM.

Multistep power consumption forecasting is smart grid electricity management's most decisive problem. Moreover, it is vital to develop operational strategies for electricity management systems in smart cities for commercial and residential users. However, an efficient electricity load forecasting model is required for accurate electric power management in an intelligent grid, leading to customer financial benefits. In this article, we develop an innovative framework for short-term electricity load forecasting, which includes two significant phases: data cleaning and a Residual Convolutional Neural Network (R-CNN) with multilayered Long Short-Term Memory (ML-LSTM) architecture. Data preprocessing strategies are applied in the first phase over raw data. A deep R-CNN architecture is developed in the second phase to extract essential features from the refined electricity consumption data. The output of R-CNN layers is fed into the ML-LSTM network to learn the sequence information, and finally, fully connected layers are used for the forecasting. The proposed model is evaluated over residential IHEPC and commercial PJM datasets and extensively decreases the error rates compared to baseline models.

Identification of the ubiquitin-proteasome pathway domain by hyperparameter optimization based on a 2D convolutional neural network.

The major mechanism of proteolysis in the cytosol and nucleus is the ubiquitin-proteasome pathway (UPP). The highly controlled UPP has an effect on a wide range of cellular processes and substrates, and flaws in the system can lead to the pathogenesis of a number of serious human diseases. Knowledge about UPPs provide useful hints to understand the cellular process and drug discovery. The exponential growth in next-generation sequencing wet lab approaches have accelerated the accumulation of unannotated data in online databases, making the UPP characterization/analysis task more challenging. Thus, computational methods are used as an alternative for fast and accurate identification of UPPs. Aiming this, we develop a novel deep learning-based predictor named "2DCNN-UPP" for identifying UPPs with low error rate. In the proposed method, we used proposed algorithm with a two-dimensional convolutional neural network with dipeptide deviation features. To avoid the over fitting problem, genetic algorithm is employed to select the optimal features. Finally, the optimized attribute set are fed as input to the 2D-CNN learning engine for building the model. Empirical evidence or outcomes demonstrates that the proposed predictor achieved an overall accuracy and AUC (ROC) value using 10-fold cross validation test. Superior performance compared to other state-of-the art methods for discrimination the relations UPPs classification. Both on and independent test respectively was trained on 10-fold cross validation method and then evaluated through independent test. In the case where experimentally validated ubiquitination sites emerged, we must devise a proteomics-based predictor of ubiquitination. Meanwhile, we also evaluated the generalization power of our trained modal via independent test, and obtained remarkable performance in term of 0.862 accuracy, 0.921 sensitivity, 0.803 specificity 0.803, and 0.730 Matthews correlation coefficient (MCC) respectively. Four approaches were used in the sequences, and the physical properties were calculated combined. When used a 10-fold cross-validation, 2D-CNN-UPP obtained an AUC (ROC) value of 0.862 predicted score. We analyzed the relationship between UPP protein and non-UPP protein predicted score. Last but not least, this research could effectively analyze the large scale relationship between UPP proteins and non-UPP proteins in particular and other protein problems in general and our research work might improve computational biological research. Therefore, we could utilize the latest features in our model framework and Dipeptide Deviation from Expected Mean (DDE) -based protein structure features for the prediction of protein structure, functions, and different molecules, such as DNA and RNA.

To Assist Oncologists: An Efficient Machine Learning-Based Approach for Anti-Cancer Peptides Classification.

In the modern technological era, Anti-cancer peptides (ACPs) have been considered a promising cancer treatment. It's critical to find new ACPs to ensure a better knowledge of their functioning processes and vaccine development. Thus, timely and efficient ACPs using a computational technique are highly needed because of the enormous peptide sequences generated in the post-genomic era. Recently, numerous adaptive statistical algorithms have been developed for separating ACPs and NACPs. Despite great advancements, existing approaches still have insufficient feature descriptors and learning methods, limiting predictive performance. To address this, a trustworthy framework is developed for the precise identification of ACPs. Particularly, the presented approach incorporates four hypothetical feature encoding mechanisms namely: amino acid, dipeptide, tripeptide, and an improved version of pseudo amino acid composition are applied to indicate the motif of the target class. Moreover, principal component analysis (PCA) is employed for feature pruning, while selecting optimal, deep, and highly variated features. Due to the diverse nature of learning, experiments are performed over numerous algorithms to select the optimum operating method. After investigating the empirical outcomes, the support vector machine with hybrid feature space shows better performance. The proposed framework achieved an accuracy of 97.09% and 98.25% over the benchmark and independent datasets, respectively. The comparative analysis demonstrates that our proposed model outperforms as compared to the existing methods and is beneficial in drug development, and oncology.

An Effective Skin Cancer Classification Mechanism via Medical Vision Transformer.

Skin Cancer (SC) is considered the deadliest disease in the world, killing thousands of people every year. Early SC detection can increase the survival rate for patients up to 70%, hence it is highly recommended that regular head-to-toe skin examinations are conducted to determine whether there are any signs or symptoms of SC. The use of Machine Learning (ML)-based methods is having a significant impact on the classification and detection of SC diseases. However, there are certain challenges associated with the accurate classification of these diseases such as a lower detection accuracy, poor generalization of the models, and an insufficient amount of labeled data for training. To address these challenges, in this work we developed a two-tier framework for the accurate classification of SC. During the first stage of the framework, we applied different methods for data augmentation to increase the number of image samples for effective training. As part of the second tier of the framework, taking into consideration the promising performance of the Medical Vision Transformer (MVT) in the analysis of medical images, we developed an MVT-based classification model for SC. This MVT splits the input image into image patches and then feeds these patches to the transformer in a sequence structure, like word embedding. Finally, Multi-Layer Perceptron (MLP) is used to classify the input image into the corresponding class. Based on the experimental results achieved on the Human Against Machine (HAM10000) datasets, we concluded that the proposed MVT-based model achieves better results than current state-of-the-art techniques for SC classification.

A Deep-Learning Model for Real-Time Red Palm Weevil Detection and Localization.

Background and motivation: Over the last two decades, particularly in the Middle East, Red Palm Weevils (RPW, Rhynchophorus ferruginous) have proved to be the most destructive pest of palm trees across the globe. Problem: The RPW has caused considerable damage to various palm species. The early identification of the RPW is a challenging task for good date production since the identification will prevent palm trees from being affected by the RPW. This is one of the reasons why the use of advanced technology will help in the prevention of the spread of the RPW on palm trees. Many researchers have worked on finding an accurate technique for the identification, localization and classification of the RPW pest. This study aimed to develop a model that can use a deep-learning approach to identify and discriminate between the RPW and other insects living in palm tree habitats using a deep-learning technique. Researchers had not applied deep learning to the classification of red palm weevils previously. Methods: In this study, a region-based convolutional neural network (R-CNN) algorithm was used to detect the location of the RPW in an image by building bounding boxes around the image. A CNN algorithm was applied in order to extract the features to enclose with the bounding boxes-the selection target. In addition, these features were passed through the classification and regression layers to determine the presence of the RPW with a high degree of accuracy and to locate its coordinates. Results: As a result of the developed model, the RPW can be quickly detected with a high accuracy of 100% in infested palm trees at an early stage. In the Al-Qassim region, which has thousands of farms, the model sets the path for deploying an efficient, low-cost RPW detection and classification technology for palm trees.

An Efficient and Effective Deep Learning-Based Model for Real-Time Face Mask Detection.

Since December 2019, the COVID-19 pandemic has led to a dramatic loss of human lives and caused severe economic crises worldwide. COVID-19 virus transmission generally occurs through a small respiratory droplet ejected from the mouth or nose of an infected person to another person. To reduce and prevent the spread of COVID-19 transmission, the World Health Organization (WHO) advises the public to wear face masks as one of the most practical and effective prevention methods. Early face mask detection is very important to prevent the spread of COVID-19. For this purpose, we investigate several deep learning-based architectures such as VGG16, VGG19, InceptionV3, ResNet-101, ResNet-50, EfficientNet, MobileNetV1, and MobileNetV2. After these experiments, we propose an efficient and effective model for face mask detection with the potential to be deployable over edge devices. Our proposed model is based on MobileNetV2 architecture that extracts salient features from the input data that are then passed to an autoencoder to form more abstract representations prior to the classification layer. The proposed model also adopts extensive data augmentation techniques (e.g., rotation, flip, Gaussian blur, sharping, emboss, skew, and shear) to increase the number of samples for effective training. The performance of our proposed model is evaluated on three publicly available datasets and achieved the highest performance as compared to other state-of-the-art models.

An Efficient Lightweight Hybrid Model with Attention Mechanism for Enhancer Sequence Recognition.

Enhancers are sequences with short motifs that exhibit high positional variability and free scattering properties. Identification of these noncoding DNA fragments and their strength are extremely important because they play a key role in controlling gene regulation on a cellular basis. The identification of enhancers is more complex than that of other factors in the genome because they are freely scattered, and their location varies widely. In recent years, bioinformatics tools have enabled significant improvement in identifying this biological difficulty. Cell line-specific screening is not possible using these existing computational methods based solely on DNA sequences. DNA segment chromatin accessibility may provide useful information about its potential function in regulation, thereby identifying regulatory elements based on its chromatin accessibility. In chromatin, the entanglement structure allows positions far apart in the sequence to encounter each other, regardless of their proximity to the gene to be acted upon. Thus, identifying enhancers and assessing their strength is difficult and time-consuming. The goal of our work was to overcome these limitations by presenting a convolutional neural network (CNN) with attention-gated recurrent units (AttGRU) based on Deep Learning. It used a CNN and one-hot coding to build models, primarily to identify enhancers and secondarily to classify their strength. To test the performance of the proposed model, parallels were drawn between enhancer-CNNAttGRU and existing state-of-the-art methods to enable comparisons. The proposed model performed the best for predicting stage one and stage two enhancer sequences, as well as their strengths, in a cross-species analysis, achieving best accuracy values of 87.39% and 84.46%, respectively. Overall, the results showed that the proposed model provided comparable results to state-of-the-art models, highlighting its usefulness.

Abnormal Activity Recognition from Surveillance Videos Using Convolutional Neural Network.

Background and motivation: Every year, millions of Muslims worldwide come to Mecca to perform the Hajj. In order to maintain the security of the pilgrims, the Saudi government has installed about 5000 closed circuit television (CCTV) cameras to monitor crowd activity efficiently. PROBLEM: As a result, these cameras generate an enormous amount of visual data through manual or offline monitoring, requiring numerous human resources for efficient tracking. Therefore, there is an urgent need to develop an intelligent and automatic system in order to efficiently monitor crowds and identify abnormal activity. METHOD: The existing method is incapable of extracting discriminative features from surveillance videos as pre-trained weights of different architectures were used. This paper develops a lightweight approach for accurately identifying violent activity in surveillance environments. As the first step of the proposed framework, a lightweight CNN model is trained on our own pilgrim's dataset to detect pilgrims from the surveillance cameras. These preprocessed salient frames are passed to a lightweight CNN model for spatial features extraction in the second step. In the third step, a Long Short Term Memory network (LSTM) is developed to extract temporal features. Finally, in the last step, in the case of violent activity or accidents, the proposed system will generate an alarm in real time to inform law enforcement agencies to take appropriate action, thus helping to avoid accidents and stampedes. RESULTS: We have conducted multiple experiments on two publicly available violent activity datasets, such as Surveillance Fight and Hockey Fight datasets; our proposed model achieved accuracies of 81.05 and 98.00, respectively.