Prevalence of Escherichia coli O157:H7 isolated from fecal samples of diarrheic camels in Tunisia.

Shiga­toxin­producing E. coli (STEC) is a foodborne pathogen associated with outbreaks worldwide that can be identified in the feces and in the meat of food­producing animals. Our study aimed to evaluate the incidence of E. coli O157:H7 in the feces of diarrheic camels (Camelus dromedarius) in Tunisia. From January 2018 to April 2019, 120 unduplicated fecal samples were obtained from diarrheic camels located in southern Tunisia. Non­sorbitol­fermenting colonies were confirmed as E. coli O157 via latex agglutination test and were screened for the presence of rfbEO157, fliCH7, stx1, stx2, eaeA, and ehxA genes by PCR. All isolates were examined for their susceptibility to 21 antibiotics. Of the 70 E. coli isolates that were recovered from 120 diarrheic camels, 4 (5.7%) were identified as STEC O157:H7. All isolates harbored ehxA and eae genes. Shiga toxin genes stx2 and stx1 were present in 50% and 25% of isolates, respectively. All E. coli O157:H7 isolates were sensitive to amoxicillin/clavulanic acid, cefotaxime, cefepime, aztreonam, colistin, and sulfamethoxazole­trimethoprim. All isolates belonged to the phylogroup E. This is the first report of E. coli O157:H7 isolates from diarrheic camels in Tunisia with a prevalence of 4 isolates (3.3%) amongst 120 fecal samples. This study supports the necessity for a platform purposed for regular screening and surveillance programs in food­producing animals and meat products, to perform early and rapid identification of food­borne pathogens.

Serological survey and associated risk factors' analysis of Trypanosomiasis in camels from Southern Tunisia.

Surra (Trypanosoma evansi infection) is one of the main causes of dromedary (Camelus dromedarius) abortion, besides generating severe economic losses in herds. A sero-epidemiological survey was carried out between December 2018 and December 2019 in Southern Tunisia to estimate the seroprevalence of Trypanosoma evansi infection in camels and to determine its possible associated risk factors. Two-stage sampling was conducted to select breeders and camels targeted in our study. A total of 1205 blood samples were collected from 277 randomly selected farms belonging to six governorates of southern Tunisia. Sera were tested with the card agglutination test for Trypanosoma evansi (CATT/T. evansi) to detect the presence of anti-Trypanosoma. evansi antibodies. The overall individual and herd seroprevalence were 30.8% (95%CI 27.9-33.1%), 64.9% (95%CI 61.7-73), respectively. The seroprevalence of T. evansi infection both at the animal (26.2% (95%CI 21.4-30.9%) and herd level (84.4 (95%CI 76.3-92.5)) was higher in Kebili than in other governorates (P = 0.003). At the animal level, the infection rate with T. evansi was significantly associated to the age group among camels (P = 0.0008), production system (P = 0.006), bioclimatic stage (P = 0.02), and herd size (P = 0.04) in the univariable analysis. Multivariable logistic regression indicated that only age group and herd size were potential risk factors associated with Trypanosoma evansi infection. However, no significant variation of the seroprevalence of T. evansi with the sex of camels, farm type, and previous trypanocidal treatment were detected (P > 0.05). The findings of this study are crucial for this disease surveillance and control. Further investigations on the efficacy of the treatment against surra are needed to explain the persistence of the disease in the south of Tunisia.