Metabolic adjustments in neonatal dwarf and normal-sized goat kids: Relationship between serum metabolites and body size.

The relationship between body size and metabolism of goats remains poorly studied. The study evaluated the neonatal metabolic adjustments and elucidated the relationship between serum metabolites and body size in 39 single-born dwarf and normal-sized goat kids. Body weight, length and height of kids were recorded at birth and blood samples were collected from the dwarf and normal-sized (Red Sokoto and Sahel) goats on Days 0 (birth), 3, 10 and 20, postnatal. Also, the body mass index (BMI) was calculated and the concentration of metabolic markers was determined. Results revealed that values of BMI, body weight, length and height were lowest (P < 0.01) in the dwarf, followed by values in Red Sokoto kids, while the Sahel kids had the highest (P < 0.01) values. Conversely, the concentration of triglyceride at birth was highest (P < 0.05) in the dwarf, moderate in Red Sokoto and lowest in Sahel goats. Similarly, the Sahel goat kids had the lowest neonatal (P < 0.05) concentration of serum cholesterol. Neonatal concentrations of serum albumin and urea were higher in Sahel than Red Sokoto (P < 0.05) and the dwarf (P > 0.05) goats. Concentration of serum albumin was lower (P < 0.05) at birth, but significantly increased later, while values of serum urea concentration were higher (P < 0.05) at birth, but significantly decreased in subsequent postnatal days. Unlike the BMI, birth weight showed significant negative (P < 0.05) correlation with the concentration of most serum metabolites, especially triglyceride, which showed negative correlation at birth and in subsequent postnatal days. We concluded that dwarfism or small body size is associated with high serum triglyceride in single-born neonatal goats, and this is probably due to the accumulation of body energy reserve in the form of body fat to compensate for lower body tissue mass.

Role of maternal variables on the development of neonatal hypoglycaemia and influence of neonatal hypoglycaemia on performance of goat kids.

The study evaluated the influence of maternal variables (age, body weight and body mass index; BMI) during mating on the development of hypoglycaemia and investigated whether hypoglycaemia at birth impairs thermoregulation, metabolism, body weight gain and immunoglobulin concentration in neonatal goat kids. Post-kidding, the kids born with hypoglycaemia (n = 19) and normoglycaemia (n = 19) were immediately identified and postnatal blood samples, body weight and cardinal physiological variables were determined. Results revealed no significant (P < 0.05) difference in pre-mating maternal variables between dams that kidded hypoglycaemic and normoglycaemic kids. Kids born with hypoglycaemia had lower (P <0.05) blood glucose concentration from birth, until Day 2, when values became comparable between the two groups. Afternoon respiratory and pulse rates were markedly (P < 0.05) unstable in kids born with hypoglycaemia and the early postnatal rise (P < 0.05) in morning rectal temperature in both groups was accompanied by a decrease (P < 0.05) on day 20 in kids born with hypoglycaemia. Blood cholesterol and triglyceride concentrations were lower (P < 0.05) in hypoglycaemic kids and the normoglycaemic kids showed marked increase (P < 0.05) in circulating immunoglobulin concentration 24 h after birth, while age had no (P > 0.05) effect in hypoglycaemic kids. A more pronounced decrease (P < 0.05) in weekly weight gain was observed in hypoglycaemic kids. It was concluded that neonatal goat kids born with hypoglycaemia may have compromised thermoregulation, metabolism and body weight gain, and the cause of hypoglycaemia in kids may not be related to pre-mating maternal variables.

Thermoregulation in periparturient rabbit does and their neonatal kits with different litter sizes during West African winter.

The study evaluated the changes in body temperatures and hematological parameters in periparturient rabbit does and the dynamics of body surface temperature in neonatal rabbit kits during West African winter. The variables were measured in 21 rabbits, comprising periparturient (n = 12) and dry (n = 9) does, and after kindling, the kits were grouped based on litter size, into small (4 kits per litter; n = 24) and large (5-7 kits per litter; n = 34) litter. Results revealed that all body temperatures of the does were lower (p < 0.05) during gestation compared with lactation period or dry does in all hours of the day, and the PCV was significantly higher in lactating than dry does. At birth, small litter kits had higher (p < 0.05) body weight and morning body surface temperature than the large litter kits. The large litter kits showed distinct (p < 0.05) diurnal variation in body surface temperature from birth, while diurnal variation in the small litter kits was absent (P > 0.05) in the first three days after birth. It was concluded that pregnant rabbits and large litter kits may have poor thermoregulation during West African winter, and thus, the need for special protective housing to improve productivity.

Neonatal thermoregulation and dynamics of serum thyroid hormones in tropical breeds of goat kids.

The study was aimed at evaluating the concentration of serum thyroid hormones and diurnal trends in rectal and body surface temperatures in neonatal buck-kids and doelings. Rectal and body surface (eye, head and trunk) temperatures as well as the differences between rectal and surface (DBRS) temperatures were determined in 39 kids, having equal number of Red Sokoto, Sahel and West African Dwarf (WAD) goats during daytime nadir (07:00 h) and zenith (13:00 h) in ambient temperatures. All variables and blood samples were obtained from birth to Day 20, post-natal. Results revealed significantly (P < 0.05) lower values of morning and afternoon rectal temperatures at birth with subsequent post-natal increase, attaining higher stable values after Day 2. Morning head surface temperature decreased significantly after birth, whereas, DBRS temperatures were lower at birth and increased in subsequent days. From birth, diurnality was distinct (P < 0.05) in all body surface and DBRS temperatures, while, diurnality in rectal temperature was only observed on Days 1-8 (P < 0.05). All body temperatures (rectal, eye, head and trunk) recorded during the afternoon hours in the late neonatal life (Days 10-20) were significantly higher in WAD than Red Sokoto and Sahel kids. Buck-kids had higher (P < 0.05) values of morning rectal temperature than doelings. Circulating concentration of tri-iodothyronine was significantly lower in Sahel than Red Sokoto and WAD goat kids, whereas, the values of thyroxin were lower (P < 0.05) in Red Sokoto than Sahel and WAD kids. Similarly, lower (P < 0.05) values of tri-iodothyronine were recorded in buck-kids, while a higher (P < 0.05) level of thyroxin was recorded in doelings. In conclusion, neonatal adjustment in body temperatures of tropical breeds was influenced by thyroid hormone concentration, and the feeble cutaneous thermoregulation atbirth, contributed significantly to heat loss and gain during the morning and afternoon hours, respectively.

Comparative effect of dietary supplements on the performance and severity of experimental Eimeria tenella infection in broiler chickens.

The effect of the dietary supplements, sugarcane molasses (prebiotic), Antox® (probiotic) and Enflorax® (synbiotic), on gut health, performance and severity of infection with Eimeria tenella in broiler chickens was evaluated in this study. Ninety-day-old broiler chicks were divided into six groups (A, B, C, D, E and F). Groups B to F were challenged with Eimeria tenella (2.0 × 104 oocysts/chick). Groups C, D and E were supplemented from day old with prebiotic, probiotic and synbiotic, respectively, while F was administered amprolium. Groups A and B served as negative and positive controls, respectively. Feed intake and performance parameters were assessed weekly for each group. After infection clinical signs, morbidity and mortality rates were monitored alongside oocyst output, gross and microscopic caecal lesions. Infected chickens exhibited clinical signs 4 days post infection (dpi) with 100% morbidity in all infected groups. Infected groups showed significant (P < 0.05) drop in feed intake and weight gain from 3 to 5 weeks of age. Feed conversion ratio was highest in B but lower in the supplemented groups. Oocyst output in faeces were significantly lower (P < 0.05) in the supplemented groups compared with B. Macroscopic lesion scores 7 dpi were significantly lower in the supplemented groups compared with B, though group F had the lowest mean score. Histopathological examination of caeca tissues showed milder lesions in the supplemented groups. In conclusion, the supplements prebiotic, probiotic and synbiotic ameliorated the consequences of caecal coccidiosis in broiler chickens and therefore recommended for use in broiler production.

Postnatal hypoglycemia and blood glucose concentrations in neonatal tropical goat kids.

This study aimed to evaluate the influences of breed, sex, litter size, and birth weight on the incidence of postnatal hypoglycemia and blood glucose concentrations in neonatal tropical goats. A total of 160 neonatal goat kids belonging to Red Sokoto (n = 98), Sahel (n = 40), and West African Dwarf (n = 22) breeds, were categorized after birth based on glycemic status, sex, litter size, and birth weights (high or low). Blood samples were collected on postnatal days 0, 1, 2, 3, 10, and 20 to determine blood glucose concentrations. Results revealed that the incidence of hypoglycemia at birth among neonatal goat kids was 22.5% (36/160) and was significantly (P < 0.05) higher in the Sahel breed kids, twin kids, and kids born with low birth weights. After 24 hours, all hypoglycemic kids became normoglycemic but maintained lower (P < 0.05) blood glucose levels than those that were normoglycemic at birth, until day 10, when both groups had comparable blood glucose levels. In all groups, blood glucose concentrations increased significantly 24 hours postnatal, and kids with high birth weights had significantly higher values than those with low birth weights, while the buck kids had higher values than the doelings. There was a negative correlation (r = -0.31; P < 0.0003) between the blood glucose concentration of dams and those of their kids. In conclusion, the incidence of hypoglycemia in tropical goat kids was markedly influenced by breed, birth weight, and litter size. Furthermore, the hypoglycemic kids maintained lower blood glucose concentrations until day 10 postnatal, suggesting the need for relevant nutritional provisions for hypoglycemic kids during this critical period.

Thermoregulatory, oxidative stress and lipid responses in prepartum ewes administered with L-carnosine during the hot-dry season.

The aim of the study was to investigate the effects of L-carnosine administration to prepartum ewes on oxidative status, lipid profile and thermoregulatory responses in the tropics during the hot-dry season. Twenty pregnant ewes were allotted each into two groups of treated and untreated ewes. The untreated ewes received orally 10 mL of water, while treated ewes were administered orally with L-carnosine (100 mg/kg) daily during the last 3 weeks of gestation. Blood samples were obtained during pre-gestation on day 0, gestation days 136 and 143 and after lambing. Dry-bulb temperatures (DBTs) of the pen were measured once per week at 06:00, 14:00 and 18:00 h as well as the thermoregulatory responses of the ewes. Birth weights of the lambs were determined 2-3 h postpartum. The DBT (37.33 ± 1.20 °C) and temperature-humidity index (85.24 ± 1.91) were above the thermoneutral zone. The results revealed no significant effect of L-carnosine on thermoregulatory responses of ewes and on the live-birth weight of their lambs. Malondialdehyde, 8-isoprostane and triglyceride concentrations were lower (P < 0.05) in treated ewes than in untreated ewes, but superoxide dismutase, catalase and glutathione peroxidase activities were higher (P < 0.05) in treated ewes compared to untreated ewes. In conclusion, L-carnosine reduced lipo-peroxidative products and augmented antioxidant enzyme activities in prepartum ewes under hot-dry conditions.

In vivo ameliorative effects of methanol leaf extract of Lawsonia inermis Linn on experimental Trypanosoma congolense infection in Wistar rats.

The aim of this study was to investigate the ameliorative effect of Lawsonia inermis Linn used traditionally against trypanosomosis. Twenty-five adult Wistar rats of both sex were individually infected intraperitoneally (IP) with 106Trypanosoma congolense per ml of blood. Following establishment of infection, the rats were randomly divided into five groups of 5 rats each. Rats in groups I, II, and III were treated with 125, 250 and 500 mg/kg of the extract, respectively, while rats in groups IV and V were treated with 3.5 mg/kg and 2 ml/kg of diminazene aceturate (DM) once and physiological buffered saline, respectively. All treatments except DM were given orally for 7 days IP. The antitrypanosomal effect of the plant was assessed by observing the level of parasitaemia daily, packed cell volume (PCV) weekly, erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration on day 21. Phytochemical screening of the extract revealed the presence of alkaloids, carbohydrates, triterpenes, steroids, cardiac glycosides, saponins, tannins and flavonoids. The extract significantly (P < 0.05) reduced levels of parasitaemia at 250 mg/kg. PCV was higher (P > 0.05) in extract treated groups but significantly higher (P < 0.05) in group II at week 2 when compared to group V. Rats in group II had significantly lower values of EOF and MDA when compared with groups IV and V. Thus, the leaf of L. inermis has in addition to an antitrypanosomal effect against T. congolense in rats, an attenuating effect on the trypanosomosis pathology probably mediated via protection of the erythrocyte membrane against trypanosome-induced oxidative damage to the erythrocytes.