PKR-like ER kinase (PERK) Haplotypes Are Associated with Depressive Symptoms in People with HIV.

Background: Depression is a debilitating and difficult-to-treat condition in people with HIV (PWH) despite viral suppression on antiretroviral therapy (ART). Depression is associated with activation of the PKR-like ER kinase (PERK) pathway, which regulates protein synthesis in response to metabolic stress. We evaluated common PERK haplotypes that influence PERK expression in relation to depressed mood in PWH. Methods: PWH from 6 research centers were enrolled in the study. Genotyping was conducted using targeted sequencing with TaqMan. The major PERK haplotypes A, B, and D were identified. Depressive symptom severity was assessed using the Beck Depression Inventory-II (BDI-II). Covariates including genetically-defined ancestry, demographics, HIV disease/treatment parameters and antidepressant treatments were assessed. Data were analyzed using multivariable regression models. Results: A total of 287 PWH with a mean (SD) age of 57.1±7.8 years were enrolled. Although the largest ethnic group was non-Hispanic white (n=129, 45.3%), African-American (n=124, 43.5%) and Hispanic (n=30, 10.5%) made up over half the sample. 20.3% were female and 96.5% were virally suppressed. Mean BDI-II was 9.6±9.5, and 28.9% scored above the cutoff for mild depression (BDI-II>13). PERK haplotype frequencies were AA57.8%, AB25.8%, AD 10.1%, and BB4.88%. PERK haplotypes were differentially represented according to genetic ancestry (p=6.84e-6). BDI-II scores were significantly higher in participants with the AB haplotype (F=4.45, p=0.0007).This finding was robust to consideration of potential confounds. Conclusion: PERK haplotypes were associated with depressed mood in PWH.Consequently, pharmacological targeting of PERK-related pathways might amelioratedepression in PWH.

Focusing anomalies with binary diffractive optical elements.

Binary diffractive optics have been extensively studied to date as tools for arbitrary laser beam shaping and experimentally implemented with etched transparent optics and spatial light modulators. Here we demonstrate that a simple one-step binary optic is able to enhance the intensity of a focused beam, displaying some counterintuitive focusing anomalies. We explain these effects by considering the optical aberrations in binary diffractive optics and outline how this may be exploited for further improvements in refractive/diffractive combinations for super-resolution microscopy.

Toll-like receptor 2 ligand, lipoteichoic acid is inhibitory against infectious laryngotracheitis virus infection in vitro and in vivo.

Lipoteichoic acid (LTA) is one of the pathogen associated molecular patterns (PAMPs) that activates toll-like receptor (TLR)2-cluster of differentiation (CD)14 signalling pathway. This recognition elicits antiviral responses that have been recorded against viruses of mammals although such responses have not been characterized adequately against avian viruses. In this investigation, we characterized the LTA induced antiviral responses against infectious laryntotracheitis virus (ILTV) infection in vitro and in vivo. We found that LTA is capable of up regulating mRNA expression of innate proteins in macrophages such as MyD88, iNOS and IL-1ß and reduces the ILTV plaques in vitro. Similarly, we found that LTA treatment of embryonic day 18 (ED18) eggs can lead to the antiviral response against pre-hatch ILTV infection in vivo and is associated with expansion of macrophage populations and expression of IL-1ß and MyD88 in the lung. The data highlight that LTA can be a potential innate immune stimulant that can be used against ILTV infection in chickens.

Photonic molecules: tailoring the coupling strength and sign.

We demonstrate a large tuning of the coupling strength in Photonic Crystal molecules without changing the inter-cavity distance. The key element for the design is the "photonic barrier engineering", where the "potential barrier" is formed by the air-holes in between the two cavities. This consists in changing the hole radius of the central row in the barrier. As a result we show, both numerically and experimentally, that the wavelength splitting in two evanescently-coupled Photonic Crystal L3 cavities (three holes missing in the ΓK direction of the underlying triangular lattice) can be continuously controlled up to 5× the initial value upon ∼ 30% of hole-size modification in the barrier. Moreover, the sign of the splitting can be reversed in such a way that the fundamental mode can be either the symmetric or the anti-symmetric one without altering neither the cavity geometry nor the inter-cavity distance. Coupling sign inversion is explained in the framework of a Fabry-Perot model with underlying propagating Bloch modes in coupled W1 waveguides.

High quality beaming and efficient free-space coupling in L3 photonic crystal active nanocavities.

We report on far-field measurements of L3 photonic crystal (PhC) cavities with high quality beaming. This is achieved by means of the so-called "band folding" technique, in which a modulation of the radius of specific holes surrounding the cavity is introduced. Far-field patterns are measured from photoluminescence of quantum wells embedded in the PhC. A very good agreement between experimental results and simulated radiation patterns has been found. Laser effect is demonstrated in the beaming cavity with a threshold comparable to the regular one. In addition, free-space input coupling to this cavity has been achieved. In order to fully analyze the coupling efficiency, we generalize the approach developed in S. Fan, et al., [J. Opt. Soc. Am. A 20, 569 (2003)], relaxing the hypothesis of mirror symmetry. The obtained coupling efficiencies are about 15% with quality factors (Q) exceeding 10(4). These results further validate the "folding" technique on L3 cavities for nanocavity realization with efficient free-space coupling and high Q factors.

Determination of polycyclic aromatic hydrocarbons and their oxy-, nitro-, and hydroxy-oxidation products.

A sensitive method has been developed for the trace analysis of PAHs and their oxidation products (i.e., nitro-, oxy-, and hydroxy-PAHs) in air particulate matter (PM). Following PM extraction, PAHs, nitro-, oxy-, and hydroxy-PAHs were fractionated using solid phase extraction (SPE) based on their polarities. Gas chromatography-mass spectrometry (GC-MS) conditions were optimized, addressing injection (i.e., splitless time), negative-ion chemical ionization (NICI) parameters, i.e., source temperature and methane flow rate, and MS scanning conditions. Each class of PAH oxidation products was then analyzed using the sample preparation and appropriate ionization conditions (e.g., nitro-PAHs exhibited the greatest sensitivity when analyzed with NICI-MS while hydroxy-PAHs required chemical derivatization prior to GC-MS analysis). The analyses were performed in selected-ion-total-ion (SITI) mode, combining the increased sensitivity of selected-ion monitoring (SIM) with the identification advantages of total-ion current (TIC). The instrumental LODs determined were 6-34 pg for PAHs, 5-36 pg for oxy-PAHs, and 1-21 pg for derivatized hydroxy-PAHs using electron ionization (GC-EI-MS). NICI-MS was found to be a useful tool for confirming the tentative identification of oxy-PAHs. For nitro-PAHs, LODs were 1-10 pg using negative-ion chemical ionization (GC-NICI-MS). The developed method was successfully applied to two types of real-world PM samples, diesel exhaust standard reference material (SRM 2975) and wood smoke PM.

[Polytrauma: a therapeutic and logistical approach]. / Le polytraumatisé : approche thérapeutique et logistique.

This work aims to define a strategy for the management of polytrauma based on findings from a review of the recent literature. After a definition of terminology, we address the modes of injury, pathophysiology, and the different phases in the management of polytrauma. We assess the results of current management of polytrauma in developed countries and stress the need for improvement in trauma systems and therapeutic interventions in order to achieve better results.

Anodized aluminum wire as a solid-phase microextraction fiber.

The efficiency of anodized aluminum wire was investigated as a new fiber for solid-phase microextraction (SPME). Aluminum wires were anodized by direct current in a solution of sulfuric acid at room temperature and were conditioned at 300 degrees C for 30 min. These fibers were used for the extraction of some aliphatic alcohols, BTEX, and petroleum products from gaseous samples. The extracted analytes were transferred to a GC injector using an (inhouse-designed) SPME syringe that also allowed for an easy change of SPME fibers. The results obtained prove the ability of anodized aluminum wire as a new fiber for sampling of organic compounds from gaseous samples. This behavior is due most probably to the porous layer of aluminum oxide, which is formed on the metal surfaces. In this work, the optimum conditions for the preparation and conditioning of fibers and the extraction of analytes from gaseous samples were obtained. In the optimum conditions, one fiber was used in several equal analyses and the relative standard deviations were below 5% (n = 5). However, fiber-to-fiber reproducibility was 8% (n = 5). This fiber is firm, inexpensive, and durable and can be prepared simply.