RESUMO
3 alpha-Hydroxysteroid dehydrogenase (SDH) quantifies ursodeoxycholic acid, 3 alpha,12 alpha-diol-7-one-5 beta-cholanoic acid, 3 alpha-ol-7-one-5 beta-cholanoic acid, and 3 alpha,7 alpha-diol-12-one-5 beta-cholanoic acid in a manner similar to the more commonly measured bile acids, exemplified by taurocholic acid. The type of oxygen function at the 7 or 12 position and its orientation at the 7 position has no effect on the rate of reaction of the enzyme. Discrepancies in the glycine/taurine ratios of patient intestinal aspirates containing ursodeoxycholic acid and oxo bile acids, obtained by SDH and gas-liquid chromatographic methods are not a result of the enzyme assay procedure.
Assuntos
3-Hidroxiesteroide Desidrogenases , Ácidos Cólicos/análise , Ácido Desoxicólico/análogos & derivados , Ácido Ursodesoxicólico/análise , Bile/análise , Ácidos e Sais Biliares/análise , Ácido Quenodesoxicólico/análogos & derivados , Ácido Quenodesoxicólico/análise , Cromatografia Gasosa , Ácido Desoxicólico/análise , Glicina/análise , Humanos , Intestinos/análise , Ácido Litocólico/análogos & derivados , Ácido Litocólico/análise , Taurina/análiseAssuntos
Catecolaminas/urina , Cromatografia Gasosa , Amidas , Cromatografia Líquida , Dopamina/urina , Humanos , Metanefrina/urina , Métodos , Normetanefrina/urina , Polímeros , Tiramina/urinaRESUMO
Proximal intestinal bile acids have been studied in 14 ambulatory patients with varying degrees of azotaemia. When compared with normal subjects, the azotaemic patients showed a significant decrease in deoxycholic acid. Analysis of faecal bile acids of a patient with low intestinal deoxycholic acid also showed low deoxycholic acid with a preponderance of primary bile acids, and contrast with faecal bile acids of a normal subject and a patient with normal intestinal deoxycholic acid. It is suggested that impairment of deconjugation or 7alpha-dehydroxylation might be contributing to the low deoxycholic acid observed in azotaemic patients. Unusual bile acids: ursodeoxycholic acid, 3-hydroxy-7-keto-cholanic acid, and 3,12-dihydroxy-7-keto-cholanic acid were also noted in intestinal aspirates of azotaemic patients. The presence of these bile acids in conjunction with low deoxycholic acid correlates with the symptom of diarrhoea in azotaemic patients, and may contribute to the pathogenesis of diarrhoea in these patients.
Assuntos
Ácidos e Sais Biliares/metabolismo , Diarreia/etiologia , Uremia/metabolismo , Adolescente , Adulto , Idoso , Ácido Desoxicólico/metabolismo , Fezes/análise , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
A method for the quantitative determination of the total bile acid content of human intestinal aspirates is presented. This method was applied to duodenal samples from ten normal subjects and distal jejunal samples from seven of these subjects. Enzymatic and radioactive monitoring of the procedures utilized yielded recoveries of 89 to 100 percent of bile acids.
Assuntos
Ácidos e Sais Biliares/análise , Secreções Intestinais/análise , Adulto , Cromatografia Gasosa , Duodeno/análise , Feminino , Humanos , Jejuno/análise , Masculino , Métodos , Pessoa de Meia-Idade , Ácido Taurocólico/análiseRESUMO
The conjugated bile acid pattern was evaluated in intestinal aspirates of five normal subjects and five patients known to have an abnormal bile acid distribution. The glycine/taurine (G/T) ratios for total bile acids were determined by gas liquid chromatography (GLC) and by enzymatic assay using 3-hydroxysteroid dehydrogenase (STDH). By both methods G/T ratios in normal samples approximated 3.0 as previously reported. A discrepancy between ratio values obtained by the two methods was found in patient samples. It is suggested that the presence of keto bile acids in patient aspirates correlates with this discrepancy. The G/T ratios for individual bile acids were obtained by GLC. The G/T ratio for cholic acid was higher than previously reported. Recovery studies showed that a loss of taurocholic acid in the extraction procedure employed did not account for the high G/T-cholic acid.
