New concept of schistosomiasis lesions of urinary bladder versus development of bladder cancer.

Schistosomiasis haematobium precancerous lesions of urinary bladder included group 1: preneo-plastic non papillary (flat) lesions [reactive atypia, flat hyperplasia, keratinizing squamous metaplasia, glandular metaplasia, dysplasia, carcinoma in-situ (CIS)] and group 2 preneoplastic papillary lesions (typical papillary hyperplasia, atypical papillary hyperplasia, papilloma). The present work studied the content of the schistosomiasis precancerous lesions of the urinary bladder using image analyzer, thereby shedding more light on the significance on the early diagnosis of the high risky group. A total of 140 previously diagnosed schistosomiasis urinary bladder lesions (124 precancerous and 16 cancers as controls) were stained by H & E, and Feulgen stain. The mean SPF was high in CIS (22.7), dysplasia (19.6) & low in atypia (7.91). DNA ploidy, non-diploidy DNA was high in CIS (53%), papilloma, dysplasia and atypical papillary hyperplasia than flat hyperplasia, glandular metaplasia, keratinizing squamous metaplasia and typical papillary hyperplasia that were diploid indicating more tendency and aggressiveness of the first group to turn malignant than the second group.

Q fever (Coxiella burnetii) among man and farm animals in North Sinai, Egypt.

Antibodies against Coxiella burnetii were estimated among sheep, goats and camels (190), their owners (150 patients with pyrexia of unknown origin) and 30 normal individuals in North Sinai over the 2006 by indirect immounofluorescence assay. Nested polymerase chain reaction was used to detect Com-1 gene (genetic target of C. burnetii) encoding a 27-kDa outer membrane protein in the samples. C. burnetii IFA antibodies (IgM & IgG) in patients were 8 (5.3%) and a healthy control (3.3%). The overall was 9 of 180 (5.0%). C. burnetii IgM were detected in 3/150 (2%) patients with positive genome, while IgG were detected in 5/150 patients, only the three who had IgM and IgG had positive genome suffered high fever. C. burnetii antibodies were detected in 20 (22.5%), 12 (16.8%) & 4 (13.3%) of sheep, goats, camels, which total 36/190 (18.9%). The positive genome of these IFA positive animals was 10 (50.0%), 4 (33.3%) & zero (0.0%) respectively. On the other hand, Rhipicephalus sanguineus (dog tick) and Dermacentor andersoni (wood tick) were identified on some Q fever infected animals. The results were discussed.

Natural infection of fresh and brackish water fish with heterophyid encysted metacercariae in Dakahlia Governorate, Egypt.

The encysted metacercariae (EMC) of heterophyids in the fish species; Mugil caphalus, M. capito, M. auratus, Oreochromis n. niloticus (=Tilapia nilotica), and Tilapia zillii trapped brackish and fresh water fishes were isolated by the artificial digestion method and identified. The EMC by morphometric analysis proved to be seven phenotypical distinct types. Cross-matched seven groups of clean laboratory bred puppies each was experimentally infected with a distinct EMC type. The recovered adult flukes were compatible to seven species of six genera of family Heterophyidae. These were Heterophyes heterophyes, H. aequalis, Pygidiopsis genata, Haplorchis yokogawai, Prohemostomumn vivax, Phagicola sp. and Stictodora sp. The EMC of the seven heterophyids were recovered from all the brackish water fishes, M. caphalus, M. capito, M. auratus, O. niloticus and T. zillii. On the other hand, the EMC of P. genata, Haplorchis yokogawai and Phagicola sp. were recovered only from the fresh water fish, O. niloticus and T. zillii. All the EMC was re-described and photographed.