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J Biophotonics ; 11(6): e201700213, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29457696

RESUMO

Based on the modal analysis method, we developed a model that describes the output beam of a diode-pumped solid state (DPSS) laser emitting a multimode beam. Measuring the output beam profile in the near field and at the constructed far field the individual modes, their respective contributions, and their optical parameters are determined. Using this information, the beam is optically reshaped into a quasi-Gaussian beam by the interference and superposition of the various modes. This process is controlled by a mode modulator unit that includes different meso-aspheric elements and a soft-aperture. The converted beam is guided into a second optical unit comprising achromatic-aspheric elements to produce a thin light sheet for ultramicroscopy. We found that this light sheet is markedly thinner and exhibits less side shoulders compared with a light sheet directly generated from the output of a DPSS multimode laser.


Assuntos
Lasers de Estado Sólido , Imagem Óptica/instrumentação , Animais , Encéfalo/citologia , Drosophila melanogaster , Camundongos , Neoplasias/diagnóstico por imagem , Neoplasias/patologia , Distribuição Normal
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