RESUMO
OBJECTIVE: Endometrial cancer (EC) is one of the three most common gynecological cancers. Due to the lack of effective treatment for EC patients in an advanced stage, the mortality rate of EC is increasing rapidly. Hence, it is essential to seek for novel molecular therapeutic targets and biomarkers for EC. The aim of this study was to explore the role of miR-218 in the occurrence and development of EC and to investigate the possible underlying mechanism. PATIENTS AND METHODS: The expression level of miR-218 in EC tissues and cell lines was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Wound healing assay and Matrigel assay were performed to determine the migration and invasion abilities of EC cells. Meanwhile, the potential targets of miR-218 were predicted by bioinformatics analysis and confirmed by Luciferase reporter gene assay. In addition, the protein expression level of Adducin 2 (ADD2) was assessed by Western blotting analysis. RESULTS: QRT-PCR results revealed that miR-218 was significantly downregulated in EC tissues and cell lines. Wound healing assay and Matrigel assay demonstrated that miR-218 suppressed the migration and invasion abilities of EC cells. Online prediction databases predicted that ADD2 was a direct target of miR-218, which was verified by Luciferase reporter gene assay. Rescue experiments further validated that miR-218 could serve as a carcinoma suppressor by negatively regulating ADD2 expression in EC. CONCLUSIONS: In the present study, we elucidated that miR-218 served as a tumor suppressor in EC by negatively regulating ADD2. This might bring a novel insight into new molecular therapeutic targets and biomarkers for EC.
Assuntos
Proteínas do Citoesqueleto/metabolismo , Neoplasias do Endométrio/patologia , MicroRNAs/metabolismo , Regiões 3' não Traduzidas , Antagomirs/metabolismo , Sequência de Bases , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Proteínas do Citoesqueleto/antagonistas & inibidores , Proteínas do Citoesqueleto/genética , Neoplasias do Endométrio/metabolismo , Feminino , Humanos , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Alinhamento de SequênciaRESUMO
In 526 cases of trigeminal neuralgia,254 cases were male,272 were female.The age from 17-90,average age is 58.8. The shortest course of disease is 2 months,but the longest one is 39 yrs,with a mean course of 6.8 yrs.Pain in right side is more than in left side.Pain was more often involved simultaneously on first and second branch of trigeminal nerve.505 cases (96%)got the complete response after radiofrequency thermal coagulation therapy,13 cases (2.47%) were obviously advanced,while 8 cases (1.52%) were failed.The total effective rate was 98.47%.After follow up of 1-5yrs,in 518 efficacious cases,65 recurred cases (12.54%) were treated again by the same method,pain was then stopped.
