Vancomycin Resistant Enterococci Prevalence, Antibiotic Susceptibility Patterns and Colonization Risk Factors Among HIV-Positive Patients in Health-Care Facilities in Debre Berhan Town, Ethiopia.

Background: The majority of multidrug-resistant organisms found in immunocompromised patients are enterococci. The rise of vancomycin-resistant enterococci (VRE) poses a significant threat to public health. There is a scarcity of information regarding the prevalence of VRE in Ethiopia. Purpose: This study aims to determine the prevalence of VRE in fecal samples from Human Immunodeficiency Virus (HIV)-positive individuals, to identify associated factors, and to assess their susceptibility to selected commonly prescribed medications. Patients and Methods: A cross-sectional study was conducted from April 1 to July 15, 2023, on 170 HIV-positive clients at Debre Berhan Town. A pre-tested structured questionnaire was used to collect socio-demographic and clinical data. Stool sample was collected by trained health workers, and processed by standard microbiological techniques. Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing. Data entry and analysis was carried out by SPSS Version 25. Bivariate and multivariate logistic regressions were used to assess the associated factors. Variables with a p-value of <0.05 were considered to be significantly associated with the outcome variables and the results were displayed with tables. Results: From the total of 170 study participants, colonization of Enterococcus species was observed among 95 (55.9%). Vancomycin resistance was found in 13 (13.8%) of them with 95% confidence interval (CI) 7.4-22.1. History of hospitalization Adjusted Odds Ratio (AOR): 11.9 (95% CI 1.11-127.53); habit of eating uncooked food (AOR: 15.34 (95% CI 2.36-99.63)) and invasive procedures (AOR: 23.07 (95% CI 3.54-150)) were among the predictors of VRE. MDR (multidrug resistance) was observed in 83 (87.4%) of the isolates. The highest rate of resistance was observed for ampicillin with 72 (74.6%). Conclusion: Vancomycin and multidrug resistance of enterococci among HIV patients are significant in ART clinics of Debre Berhan Town. These warrant applicable infection prevention guidelines in the health facilities and health education on food hygiene.

Breastfeeding Practice of Children Age Less than Two Years in South Wollo Zone, Northeast Ethiopia.

BACKGROUND: Suboptimal breastfeeding causes substantial numbers of child morbidity and mortality in Ethiopia. This study was designed to assess suboptimal breastfeeding practice and its associated factors among children age less than two years in South Wollo, northeast Ethiopia. METHOD: A community-based cross-sectional study was conducted among 636 women-child pairs. The study participants were selected by a multi sage random sampling technique. Data were collected using a structured interviewer-administered questionnaire, then it was entered into Epi-Data version 3.1, and exported into SPSS version 24.0 for analysis. A Binary Logistic Regression Model with a backward elimination method was used to determine the association of factors and suboptimal breastfeeding practice of babies at a 95% confidence interval. RESULT: Six hundred and thirty-six participants were included with a response rate of 99.7%. The study showed that 36.3% babies age less than 2 years were received suboptimal breastfeeding. Cesarean delivery [AOR: 8.81; 95% CI (4.92-15.77)], lack of breastfeeding counseling [AOR: 3.22; 95% CI (1.93-5.36)], maternal feeding less than 5 times/day during breastfeeding [AOR: 2.01; 95% CI (1.20-3.34)], child health problems [AOR: 3.57; 95%CI (2.17-5.85)], and babies age less than 6 months [AOR:1.92; 95%CI (1.24-2.97)] were positively associated with suboptimal breastfeeding practice. CONCLUSION: Suboptimal breastfeeding practice is highly prevalent in Ethiopia. Health service-related and socio-cultural factors were associated with the high prevalence of suboptimal breastfeeding practice. Emphasis should be given to the breastfeeding practices of children age less than years in Ethiopia. Moreover, counseling regarding the importance of breastfeeding practices should be strengthened.

Assessment of risky sexual behaviours and associated factors among adolescents in Shewa Robit Town, Northeast, Ethiopia: a cross-sectional study.

Introduction: adolescents are at high risk of engaging in risky sexual behaviours that could predispose them to several health problems. Cognizant of these facts, studies addressing the root causes for risky sexual behaviours in risk areas like Shewa Robit are mandatory. Therefore, the study was conducted to assess risky sexual behaviours and its determinants in Shewa Robit town, Northeast Ethiopia. Methods: an institutional-based cross-sectional study was conducted among 349 students using quantitative and qualitative approaches. For the quantitative study, data entry and analysis was done by using Epidata-3.1 and statistical package for social sciences (SPSS)-20, respectively. Binary and multiple logistic regression analysis was used to identify factors associated with risky sexual practices. Odds ratio with 95% confidence interval (CI) was used for measuring the strength of association. Variables with P-value of <0.05 were considered statistically significant. Collected data from the qualitative study was debriefed, categorized, coded and common themes were generated manually. Finally, the findings were triangulated with quantitative findings. Results: a total of 338 respondents involved in the study. Overall, 168 (49.7%) of respondents have had risky sexual behaviour. Alcohol consumption (Adjusted odd ration (AOR)=5.01, 95%CI; 2.12-11.79), peer pressure (AOR=5.82, 95%CI; 2.97-11.41), grade level (AOR=5.82, 95%CI; 2.06-16.45), and family size (AOR=5.19, 95%CI; 2.24-12.01) were significantly associated with risky sexual behaviour. Conclusion: risky sexual behaviour in the study area was high. Several families and school-level factors were found to be major determinants of risky sexual behaviours. Public health interventions focusing on adolescent sexual health should be targeted to save those risky adolescents.

Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia.

Background: Tuberculosis (TB) is a leading cause of morbidity and mortality in Ethiopia. Investigation of the Mycobacterium tuberculosis complex (MTBC) species circulating in the Ethiopian population would contribute to the efforts made to control TB in the country. Therefore, this study was conducted to investigate the MTBC species and spoligo patterns in the Oromia region (central) of Ethiopia. Methods: A cross-sectional study design was used to recruit 450 smear positive pulmonary TB (PTB) cases from the Oromia region between September 2017 and August 2018. Mycobacteria were isolated from sputum samples on the Lowenstein Jensen (LJ) medium. Molecular identification of the isolates was performed by spoligotyping. The results of spoligotyping were transferred into a query box in the SITVIT2 database and Run TB-Lineage in the TB Insight website for the identification of spoligo international type (SIT) number and linages of the isolates, respectively. Statistical Product and Service Solutions (SPSS) 20 was applied for statistical analysis. Results: Three hundred and fifteen isolates were grouped under 181 different spoligotype patterns. The most dominantly isolated spoligotype pattern was SIT149 and it consisted of 23 isolates. The majority of the isolates were grouped under Euro-American (EA), East-African-Indian (EAI), and Indo-Oceanic (IO) lineages. These lineages consisted of 79.4, 9.8, and 9.8% of the isolates, respectively. One hundred and sixty-five of the isolates were classified under 31 clustered spoligotypes whereas the remaining 150 were singleton types. Furthermore, 91.1% of the total isolates were classified as orphan types. Clustering of spoligotypes was associated (p < 0.001) with EAI lineage. Conclusion: SIT149 and EA lineage were predominantly isolated from the Oromia region substantiating the findings of the similar studies conducted in other regions of Ethiopia. The observation of significant number of singleton and orphan spoligotypes warrants for additional genetic typing of the isolates using method(s) with a better discriminatory power than spoligotyping.

Drug Resistance Pattern of M. tuberculosis Complex in Oromia Region of Ethiopia.

PURPOSE: Multidrug resistant tuberculosis is an emerging problem in many parts of the world. The aim of this study was to determine the drug resistance pattern of Mycobacterium tuberculosis complex in Oromia Region of Ethiopia. PATIENTS AND METHODS: A cross-sectional study was conducted from Jan 2017 to June 2018 on 450 pulmonary tuberculosis patients who visited health facilities in nine administrative zones of Oromia Region. Socio-demographic characteristics and relevant clinical information were obtained using a structured questionnaire. Line Probe Assay for first and second line drugs was used to assess the pattern of drug resistance. SPSS version 20 was used for statistical analysis. RESULTS: Median age was 26 years and 240 (53.3%) patients were males. About 24% of them were previously treated for tuberculosis. Thirty-four (7.6%) were HIV co-infected. Line Probe Assay interpretable results were obtained for 387 isolates. Thirty (7.8%) were resistant to rifampicin and isoniazid and thus were multidrug resistant isolates. Among the multidrug resistant samples, three were found to be extensively drug resistant and one was pre-extensively drug resistant. Previous treatment history (AOR 9.94 (95% CI 3.73-26.51), P < 0.001) and nutritional status below normal (AOR 3.15 (95% CI 1.13-8.81), P < 0.029) were found to be associated with multidrug resistance. The chi-square tests have shown that there was a significant difference between the BCG vaccinated and the non-vaccinated in developing multidrug resistant tuberculosis at P = 0.027. CONCLUSION: The proportion of multidrug resistance is above the WHO estimate for the country, Ethiopia, and the fact that some zones were at risk of transmission of extensively drug resistant tuberculosis warrant great attention of the control program holders even though it has to be verified through the conventional method.