RESUMO
OBJECTIVE: Describtion of the case of incidental occurence of high grade serous tubal adenocarcinoma. DESIGN: Case study. SETTING: II. Department of Gynecology and Obstetrics, Medical Faculty of Comenius University and University Hospital, Bratislava. METHODS: Author observation, literature resources. RESULTS: Incidental occurrence of high grade serous tubal adenocarcinoma has been detected in patient after vaginal hysterectomy with bilateral adnexectomy and anterior colporhaphy. CONCLUSION: Case report confirms the importance of prophylactic salpingectomy in prevention of ovarian carcinoma.
Assuntos
Adenocarcinoma/cirurgia , Doenças dos Anexos/cirurgia , Neoplasias das Tubas Uterinas/cirurgia , Procedimentos Cirúrgicos em Ginecologia/métodos , Procedimentos Cirúrgicos Profiláticos , Adenocarcinoma/patologia , Doenças dos Anexos/patologia , Neoplasias das Tubas Uterinas/patologia , Feminino , Humanos , Histerectomia Vaginal , Achados Incidentais , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/cirurgia , Salpingectomia , Resultado do TratamentoRESUMO
The aim of this work was to determine the expression of the multidrug resistance (MDR) proteins, namely MDR1 (P-glycoprotein), MRP1 (multidrug resistance-related protein) and LRP (lung resistance-related protein), in 87 samples of breast carcinoma. Detection of these proteins was provided by using indirect enzymatic immunohistochemistry. Our findings were compared with the other clinical and pathological parameters: expression of Her2/neu, estrogen receptor status (ER), progesteron receptor status (PR), histological grade and regional lymph node status. For statistical analysis, non-parametric two sided Mann-Whitney-U test was used. Majority of breast carcinoma specimens show positivity for these proteins. The MDR1 and MRP1 signal was found in the cytoplasm of cancer cells. The expression of LRP was detected in the cytoplasm close to the nuclear membrane. The samples were positive for MDR1 protein in 57%, for MRP1 in 84% and for LRP in 79%. Comparing our results with other clinical and pathological parameters, negative correlation between ER, PR and MDR1 expressions and histological grading status was found. No associations were observed between the MRP1 and LRP proteins and histological grading, as well as between the expression of three MDR proteins and the other clinically relevant parameters. In conclusion, high frequency of expression of MDR proteins in breast carcinoma cells suggests, that these proteins might be an important factor of drug resistance in breast carcinoma. Nevertheless, the negative correlation between the histological grade of malignancy of tumor and the expression of ER, PR and MDR1 indicates possible influence of progressive tumor cell de-differentiation. However, this finding has to be confirmed in additional evaluations.
Assuntos
Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Proteínas Associadas à Resistência a Múltiplos Medicamentos/biossíntese , Biomarcadores Tumorais/análise , Feminino , Humanos , Imuno-Histoquímica , Metástase Linfática/patologia , Prognóstico , Receptor ErbB-2/metabolismo , Receptores de Estrogênio/metabolismo , Receptores de Progesterona/metabolismoRESUMO
Fifty lung cancer samples (41 non-small cell lung cancer-NSCLC and 9 small cell lung cancer-SCLC) were immunohistochemically analyzed for lung resistance-related protein (LRP) and multidrug resistance-associated protein 1 (MRP1) expressions which were then correlated with histopathological subtype of the tumor. To detect these proteins, monoclonal antibodies LRP-56 and MRPm6 were used. NSCLC samples were divided into two groups, adenocarcinomas (17 samples) and squamous cell carcinomas (24 samples). Four categories of LRP and MRP1 quantity were distinguished: +++ = high level--90--100% of positive cells, ++ = lower level--10--90% of positive cells, + = low level--up to 10% of positive cells, - = negative cells--0% of positive cells. Within the NSCLC group the most samples (36/41) had the similar level of LRP and MRP1. Significantly higher expression of both proteins was observed in the adenocarcinomas in comparison with squamous cell carcinomas. The lowest positive staining for LRP and MRP1 proteins has been found in SCLC. It is suggested that our finding can confirm the overall empirical clinical knowledge about much higher chemosensitivity of untreated SCLC comparing to NSCLC.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma de Células Pequenas/tratamento farmacológico , Carcinoma de Células Pequenas/genética , Resistência a Múltiplos Medicamentos , Perfilação da Expressão Gênica , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/genética , Proteínas Associadas à Resistência a Múltiplos Medicamentos/biossíntese , Anticorpos Monoclonais , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma de Células Pequenas/patologia , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/patologiaRESUMO
In this study we describe the localization of MRP1 in normal and hyperplastic thyroid tissues. To demostrate this protein we have used the enzyme imunohistochemical method with monoclonal antibody MRPm6. We have found MRP1 to be present in follicular epithelial cells of normal thyroid tissue and higher expression in the same cells of hyperplastic thyroid tissue. The brown colour of the diaminobenzidine reaction product allowed us to define the distribution of stain clearly. (Fig. 4, Ref. 16.)
