Nanomolar detection of methylparaben by a cost-effective hemoglobin-based biosensor.

This work describes the development of a new biosensor for methylparaben determination using electrocatalytic properties of hemoglobin in the presence of hydrogen peroxide. The voltammetric oxidation of methylparaben by the proposed biosensor in phosphate buffer (pH=7.0), a physiological pH, was studied and it was confirmed that methylparaben undergoes a one electron-one proton reaction in a diffusion-controlled process. The biosensor was fabricated by carbon paste electrode modified with hemoglobin and multiwalled carbon nanotube. Based on the excellent electrochemical properties of the modified electrode, a sensitive voltammetric method was used for determination of methylparaben within a linear range from 0.1 to 13µmolL(-1) and detection limit of 25nmolL(-1). The developed biosensor possessed accurate and rapid response to methylparaben and showed good sensitivity, stability, and repeatability. Finally, the applicability of the proposed biosensor was verified by methylparaben evaluation in various real samples.

Identification of the mechanical impedance at the human finger tip.

Rapid transients were applied to the outstretched human index finger tip, which resulted in motion primarily at the metacarpophalangeal (MCP) joint in extension and in abduction. A second-order linear model was fit to approximately 20 milliseconds of the force and displacement data to determine the effective mechanical impedance at the finger tip. Ranges of mass, damping, and stiffness parameters were estimated over a range of mean finger tip force (2-20 N for extension, 2-8 N for abduction). Effective translational finger tip mass for each subject was relatively constant for force levels greater than 6 N for extension, and constant throughout the abduction trials. Stiffness increased linearly with muscle activation. The estimated damping ratio for extension trials was about 1.7 times the ratio for abduction.