RESUMO
An E. coli K-12 mutant deficient in S-adenosylmethionine (SAM) synthesis, i.e ΔmetK, but expressing a rickettsial SAM transporter, can grow in glucose minimal medium if provided with both SAM and methionine. It uses the externally provided (R)-enantiomer of SAM as methyl donor to produce most but not all of its methionine, by methylation of homocysteine catalysed by homocysteine methyltransferase (MmuM). The ΔmetK cells are also altered in growth and are twice as long as those of the parent strain. When starved of SAM, the mutant makes a small proportion of very long cells suggesting a role of SAM and of methylation in the onset of crosswall formation.
Assuntos
Carbono/metabolismo , Divisão Celular , Escherichia coli K12/fisiologia , Proteínas de Escherichia coli/metabolismo , Homocisteína S-Metiltransferase/metabolismo , Metionina Adenosiltransferase/deficiência , Metionina/biossíntese , Meios de Cultura/química , Escherichia coli K12/citologia , Escherichia coli K12/genética , Escherichia coli K12/metabolismo , Glucose/metabolismo , S-Adenosilmetionina/metabolismoRESUMO
Vascular complications, mostly hematomas and false aneurysms, are rare after coronary angiography. Infectious problems are even less frequent and a mycotic aneurysm is an extremely rare complication. Our patient presented with a mycotic aneurysm of the external iliac artery following percutaneous transluminal coronary angioplasty.