Assuntos
Androstenodiona/sangue , Adulto , Androstenodiona/análogos & derivados , Androstenodiona/imunologia , Química Clínica/instrumentação , Química Clínica/métodos , Reações Cruzadas , Estudos de Avaliação como Assunto , Feminino , Humanos , Hipogonadismo/sangue , Soros Imunes , Indicadores e Reagentes , Matemática , Obesidade/sangue , Síndrome do Ovário Policístico/sangue , Radioimunoensaio/métodos , Soroalbumina Bovina , SoluçõesRESUMO
Fifty-eight samples of amniotic fluid from pregnant women between the gestation period of 34-42 weeks were analyzed for total cortisol levels. Thirty-four simulatneous maternal serum total cortisol levels were also measured. Amniotic fluid cortisol (AFC), maternal serum cortisol (MSC) and the ratio of AFC/MSC were correlated with L/S ratio. AFC alone and AFC/MSC ratios correlate with L/S ratios (r=0.36, p less than 0.01, and r=0.46, p less than 0.01, respectively). MSC and L/S ratios had no correlation. AFC/MSC had less individual variation as compared to AFC alone. The AFC/MSC could be divided by an arbitrary line at 0.1 and values less than 0.1 signify immature fetal lungs. Values of 0.1 and greater signify mature fetal lungs.
Assuntos
Líquido Amniótico/análise , Hidrocortisona/análise , Pulmão/embriologia , Feminino , Idade Gestacional , Humanos , Hidrocortisona/sangue , Recém-Nascido , Fosfatidilcolinas/análise , Gravidez , Complicações na Gravidez/metabolismo , Síndrome do Desconforto Respiratório do Recém-Nascido/etiologia , Esfingomielinas/análiseRESUMO
The literature suggests that the presence of cellular debris and sediment in amniotic fluid samples may alter the value of the lecithin/sphingomyelin (L/S) ratios, with a loss of as much as 75 percent of the surface-active lecithin1 and consequently a marked change in the L/S ratio. We wish to report our findings on 22 amniotic fluids obtained by transabdominal amniocentesis and analyzed with and without preliminary centrifugation. We find the L/S ratio is indeed unaltered by centrifugation. However, the actual amounts of lecithin and sphingomyelin appear to be lower in centrifuged samples.
Assuntos
Líquido Amniótico/análise , Fosfatidilcolinas/análise , Esfingomielinas/análise , Centrifugação , Feminino , Humanos , Masculino , GravidezRESUMO
One hundred fifty-seven pregnancies complicated by different degrees of diabetes, toxemia, and hypertension were studied with serial urinary placental estrogen determinations. A simple and fast method for total placental estrogen determination was used. The level of total estrogen excretion was related to Apgar score in cases of class B diabetes, severe toxemia, and also in moderate toxemia when estrogen excretion was falling. Mean estrogen levels did not differ as a function of severity of diabetes. Levels did differ with severity of toxemia; however, only the difference in mean estrogen excretion between mild and severe toxemia was significant. Estrogen excretion was very low in hypertension but was not related to Apgar score. This study concludes that total urinary estrogens constitute only a single parameter necessary in the management of high-risk pregnancies.
