Assuntos
Beneficência , Tomada de Decisões , Competência Mental , Nicotiana/citologia , Doenças das Plantas , Plantas Tóxicas , Vírus do Mosaico do Tabaco , Cristalografia , Efeito Citopatogênico Viral , Corpos de Inclusão Viral , Microinjeções , Microscopia Eletrônica , Vírus do Mosaico do Tabaco/crescimento & desenvolvimento , Vírus do Mosaico do Tabaco/isolamento & purificação , Vírus do Mosaico do Tabaco/patogenicidade , Cultura de VírusAssuntos
Meio Ambiente Extraterreno , Desenvolvimento Vegetal , Cloroplastos/citologia , Meios de Cultura , Técnicas de Cultura , Citoplasma , Retículo Endoplasmático , Complexo de Golgi , Microscopia Eletrônica , Mitocôndrias , Nitrogênio/análise , Oryza/crescimento & desenvolvimento , Consumo de Oxigênio , Fósforo/análise , Células Vegetais , Plantas/análise , Plantas/metabolismo , Plantas Tóxicas , Nicotiana/crescimento & desenvolvimento , Árvores/crescimento & desenvolvimento , Zea mays/crescimento & desenvolvimentoRESUMO
Activity of the enzymes phosphoriboisomerase, glucose-6-phosphate and 6-phosphogluconic acid dehydrogenases were assayed in healthy and TMV-infected roots of Nicotiana tabacum L. var. Samsun plants grown in nutrient culture. The results indicate that all enzymes were equally active in both infected and healthy tissues. Endogenous sterol concentration in TMV-infected and healthy N. tabacum leaves, stems, and roots were investigated. Sterol biosynthesis was studied by infusing mevalonic acid-2-14C (MVA-14C) into TMV-infected and healthy tissues and isolating the sterol fraction by silicic acid column chromatography. The component sterols of the total sterol fraction were identified by gas-liquid chromatography. Infection with TMV had no effect on the level of sterols in roots, stems or leaves. Healthy and TMV-infected plants were not significantly different in their ability to synthesize sterols from MVA-14C. Cholesterol, campesterol, stigmasterol, and beta-sitosterol were identified as the major constituents of the total sterol fraction from stem and leaf tissues.