Fuscumol and Geranylacetone as Pheromone Components of Californian Longhorn Beetles (Coleoptera: Cerambycidae) in the Subfamily Spondylidinae.

In field trials testing attraction of cerambycid beetles to a blend of known pheromone components plus host plant volatiles, several species in the subfamily Spondylidinae were attracted to baited traps, suggesting that one or more components of the blend might constitute their pheromones. Here, we describe laboratory and field experiments aimed at identifying the actual pheromone components produced by these species. Analysis of headspace odors collected from male Tetropium abietis (Fall) (Coleoptera: Cerambycidae) contained (S)-fuscumol as a single component, whereas Asemum nitidum (LeConte) (Coleoptera: Cerambycidae) males produced both (S)-fuscumol and geranylacetone, and Asemum caseyi (Linsley) (Coleoptera: Cerambycidae) produced only geranylacetone. In field trials testing fuscumol, fuscumol acetate, and geranylacetone as individual components or in blends, in combination with host plant volatiles, A. nitidum were attracted to blends of fuscumol and geranylacetone, T. abietis were attracted to fuscumol alone, and A. caseyi were attracted to geranylacetone alone. Fuscumol acetate did not appear to be either attractive or inhibitory. These results, along with previous catches of spondylidine species in traps baited with fuscumol, provide evidence that fuscumol and geranylacetone are likely to be relatively common pheromone structures for species in the subfamily Spondylidinae.

Volatiles from intact and Lygus-damaged Erigeron annuus (L.) Pers. are highly attractive to ovipositing Lygus and its parasitoid Peristenus relictus Ruthe.

Trap cropping and biological control can provide a sustainable means of controlling insect pests. Insects in the genus Lygus (Hemiptera: Miridae) are major pests on cotton and horticultural crops throughout the United States, and pesticide resistance within Lygus populations necessitates more sustainable long-term management techniques. Here, we explore behavioral responses of Lygus bugs (L. rubrosignatus Knight) and an introduced parasitoid, Peristenus relictus (Hymenoptera: Braconidae), to a common field edge plant, Erigeron annuus, which has the potential to serve as a trap host. Erigeron annuus is attractive to Lygus in the field, with Lygus preferentially moving to Erigeron patches compared to more abundant cotton plants. To determine the role of odor cues in mediating this attraction, we collected volatiles from E. annuus with and without Lygus damage, and then tested the attractiveness of these volatiles vs. those of cotton to Lygus females and female P. relictus wasps using Y-tube and wind tunnel bioassays. We found that undamaged E. annuus emits high concentrations of a complex volatile blend (60+ compounds), with novel compounds induced and constitutive compounds up-regulated in response to damage. Additionally, both female Lygus bugs and female P. relictus wasps are highly attracted to E. annuus volatiles over those of cotton in almost every combination of damage treatments. Our results suggest that Erigeron annuus would be an effective trap plant to control Lygus in cotton, since it is highly attractive to both the pest and its natural enemy.

Gradiometric micro-SQUID susceptometer for scanning measurements of mesoscopic samples.

We have fabricated and characterized micro-SQUID susceptometers for use in low-temperature scanning probe microscopy systems. The design features the following: a 4.6 mum diameter pickup loop; an integrated field coil to apply a local field to the sample; an additional counterwound pickup-loop/field-coil pair to cancel the background signal from the applied field in the absence of the sample; modulation coils to allow setting the SQUID at its optimum bias point (independent of the applied field), and shielding and symmetry that minimizes coupling of magnetic fields into the leads and body of the SQUID. We use a SQUID series array preamplifier to obtain a system bandwidth of 1 MHz. The flux noise at 125 mK is approximately 0.25 mu Phi 0/ sqrt Hz above 10 kHz, with a value of 2.5 mu Phi 0/ sqrt Hz at 10 Hz. The nominal sensitivity to electron spins located at the center of the pickup loop is approximately 200 muB/ sqrt Hz above 10 kHz, in the white-noise frequency region.