Biological Synthesis of Silver Nanoparticles from Lavandula mairei Humbert: Antibacterial and Antioxidant Activities.

Hospital-acquired infections involving carbapenem-resistant Acinetobacter baumannii (A. baumannii) and extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae pose significant challenges in the intensive care units. The lack of novel antimicrobial drugs amplifies the urgency to explore innovative management strategies. Nanotechnology, with its ability to generate nanoparticles possessing specific properties beneficial in drug delivery and nanomedicine, stands as a pivotal research domain. The objective of this study was to synthesize, for the first time, biologically silver nanoparticles (Ag-NPs) from Lavandula mairei Humbert (L. mairei) plant. The biosynthesized Ag-NPs were characterized by UV-visible spectral analysis, X-Ray diffraction Analysis, Fourier transform infrared spectroscopy analysis, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy. Subsequently, the antibacterial and antioxidant activities of Ag-NPs were assessed using the micro-dilution method, DPPH test and FRAP assay, respectively. The green-synthesized Ag-NPs exhibited high antibacterial activity against ESBL-producing multidrug-resistant (MDR) strains and against carbapenem-resistant and non-carbapenem-resistant strains of A. baumannii, as well as a very interesting antioxidant activity. The present study suggests that these results hold very promising for the potential application of biologically synthesized Ag-NPs from L. mairei (Ag-LM-NPs) in the invention of novel antibacterial and antioxidant agents.

Fermentative optimization and characterization of exopolysaccharides from Enterococcus faecium F58 isolated from traditional fresh goat cheese.

This study focused on optimizing the fermentation-based production of Exopolysaccharides (EPS) from Enterococcus faecium F58 initially isolated from traditional Moroccan Jben, a fresh goat cheese. Using the central composite design, yeast extract, MnSO4, and time affect EPS concentration. The highest experimental and predicted EPS production yields were 2.46 g/L ± 0.38 and 2.86 g/L, respectively. Optimal concentrations of yeast extract (4.46 g/L) and MnSO4 (0.011 g/L) were identified after 26 h at 30 °C. Characterization of EPS was conducted using SEM with EDX, XRD, and FTIR analyses. These tests revealed a specific morphology and an amorphous structure. Additionally, thermogravimetric analysis indicated adequate EPS stability up to 200 °C with anti-adhesion properties against different pathogens. This study offers valuable insights into the optimized production of EPS from Enterococcus faecium F58, which exhibits significant structural and functional properties for various applications in the food and biotechnology industries. Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-023-01424-9.

Enterococcus mundtii A2 biofilm and its anti-adherence potential against pathogenic microorganisms on stainless steel 316L.

Pathogenic bacterial biofilms present significant challenges, particularly in food safety and material deterioration. Therefore, using Enterococcus mundtii A2, known for its antagonistic activity against pathogen adhesion, could serve as a novel strategy to reduce pathogenic colonization within the food sector. This study aimed to investigate the biofilm-forming ability of E. mundtii A2, isolated from camel milk, on two widely used stainless steels within the agri-food domain and to assess its anti-adhesive properties against various pathogens, especially on stainless steel 316L. Additionally, investigations into auto-aggregation and co-aggregation were also conducted. Plate count methodologies revealed increased biofilm formation by E. mundtii A2 on 316L, followed by 304L. Scanning electron microscopy (SEM) analysis revealed a dense yet thin biofilm layer, playing a critical role in reducing the adhesion of L. monocytogenes CECT 4032 and Staphylococcus aureus CECT 976, with a significant reduction of ≈ 2 Log CFU/cm2. However, Gram-negative strains, P. aeruginosa ATCC 27853 and E. coli ATCC 25922, exhibit modest adhesion reduction (~ 0.7 Log CFU/cm2). The findings demonstrate the potential of applying E. mundtii A2 biofilms as an effective strategy to reduce the adhesion and propagation of potentially pathogenic bacterial species on stainless steel 316L.

Anti-adherence Activity of Monomicrobial and Polymicrobial Food-Derived Enterococcus spp. Biofilms Against Pathogenic Bacteria.

Enterococcus species are commensal organisms of the gastrointestinal tract and can also be isolated from traditional food products. They are used as probiotics in animals and less often in humans. This study aimed to investigate the antibacterial and anti-adhesive activities of twelve food-origin Enterococcus spp. biofilms on stainless steel AISI 316 L against foodborne pathogens, including Listeria monocytogenes CECT4032, Pseudomonas aeruginosa ATCC27853, and Escherichia coli ATCC25922. The antimicrobial and co-aggregation abilities of Enterococcus spp. were evaluated using spots-agar test and spectrophotometry aggregation assay, respectively. The anti-adhesive activity of selected strains on pathogenic bacteria was tested using serial dilution technique. Enterococci strains in planktonic mode showed strong inhibition activity against different pathogens tested with a significant difference in co-aggregation capacity. Moreover, L. monocytogenes and E. coli presented a low auto-aggregation rate compared to P. aeruginosa, which showed an amount of 11.25%. Scanning electron microscopy (SEM) revealed that biofilm biomass of Enterococcus spp. increased after 10 days. The thick layer of enterococci biofilms on AISI 316 L caused a low adhesion of L. monocytogenes, resulting in a reduction of approximately 2.8 log CFU/cm² for some selected strains. Additionally, Enterococcus monocultures' biofilms were more efficient than polymicrobial cultures (a cocktail of enterococci strains) in controlling pathogen adhesion. These results indicate that monocultures of Enterococcus spp. biofilms could be used to prevent the adhesion of pathogenic bacteria on AISI 316 L.

Anti-adhesion and antibiofilm activities of Lavandula mairei humbert essential oil against Acinetobacter baumannii isolated from hospital intensive care units.

This study aimed to assess, for the first time, the anti-adhesion and antibiofilm effects of Lavandula mairei Humbert essential oil against multidrug resistant Acinetobacter baumannii. Scanning electron microscope was used for visualizing its antibiofilm activity and the effect of this oil on surface physicochemical parameters was examined as a possible anti-adhesive target. Chemical analysis of Lavandulaa mairei essential oil showed a high content of carvacrol composition (79.12%). The oil tested exhibited antibacterial efficacy with inhibition diameters of 33 to 37.33 mm and minimum inhibitory and bactericidal concentrations of 1.56 µl ml-1. The oil inhibited adhesion by 83.66%, detach 73.30% of adherent cells and eliminated 64.02% of the biofilm compared to the untreated control. Lavandula mairei essential oil has proven its possible application as a preventive strategy by intervening in the initial adhesion of Acinetobacter baumannii to polystyrene.

Microplastics pollution in sediments of Moroccan urban beaches: The Taghazout coast as a case study.

This work describes the spatiotemporal analysis of microplastics (MPs) pollution in sediments collected on a high urbanized beach located in Taghazout coast, central Atlantic part of Morocco. The study area is mainly composed of moderately well-sorted fine sands with an average density of MPs that ranged between 915 MPs/kg in 2018 and 1448 MPs/kg in 2019. The most polluted sites were in the south part of Taghazout coast, close to facilities of where beachgoers are often found. Microplastic Pollution Index (MPPI), Microplastic Impact Coefficient (CMPI), Agglomerative Hierarchical Clustering (AHC), and Multidimensional Scaling (MDS) revealed spatiotemporal variation of MPs pollution. Thus, the principal component analysis (PCA) showed a low correlation between the sediment characteristics (i.e., grain size, sorting, skewness) and MPs densities. Overall, the outputs of this baseline recommend implementing plastic management strategies to eliminate or at least minimize the collateral effects generated by MPs pollution in sediments of urbanized beaches.

Pseudomonas aeruginosa Biofilm Removal from Two Kinds of Granite Commonly Found in Catering Kitchen.

The biofilm formation on the surfaces which are in direct contact with food products might lead to their contamination and, consequently, present serious health problems for the consumers. The goals of the present work were to study P. aeruginosa biofilm formation on two granites and to investigate the efficiency of sodium hypochlorite (NaCLO) against the same biofilm formed on these substrata using the plate count method (PCM) and epifluorescence microscopy (EP). More biofilm cells adhered to Rosa Porrino than Gris Pinhel, and the PCM method indicated that NaCLO was efficient against the biofilm installed on the Gris Pinhel at the concentration of 1.5% after 15 min of treatment, while it was not efficient against the one installed on the Rosa Porrino. By contrast, the EP showed that the biofilm persists on two granites after NaCLO treatment, at different concentrations and contact times. In addition, the surface properties of granites such as mineral composition, roughness, and physicochemical properties were determined by X-ray diffraction (XRD), scanning electron microscopy coupled with electron diffraction spectroscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDS), Fourier transform infrared (FTIR), atomic force microscopy (AFM), and contact angle measurement (CAM), respectively. The results revealed that Gris Pinhel is hydrophilic with a high roughness value and Rosa Porrino is hydrophobic with low roughness, while both of them contain the quartz, feldspar, and mica as the main dominant compositions.

Occurrence of Vibrio and Salmonella species in mussels (Mytilus galloprovincialis) collected along the Moroccan Atlantic coast.

This study reports the occurrence of different Vibrio and Salmonella species in 52 samples of Mytilus galloprovincialis collected from four sites along the Atlantic coast between Agadir and Essaouira (Anza, Cap Ghir, Imssouane and Essaouira). The level of Escherichia coli (E. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas. In this study three methods were used : AFNOR NF EN ISO 6579 V08-013 for Salmonella spp., the provisional method routinely used by several laboratories (Institut Pasteur, Paris,…) for Vibrio cholerae and Vibrio parahaemolyticus in the seafood, and the most probable number method (MPN) using Norm ISO/TS 16649-3 (2005) for E. coli. The most frequently isolated Vibrios were Vibrio alginolyticus (90.4% of samples), followed by V. cholerae non O1 non O139 (15.4%) and V. parahaemolyticus (7.7%). Salmonella spp. was found in 15% of the samples. The number of E. coli ranged between 0.2/100 g and 1.8 10(3) /100 g of mussel soft tissues. This study indicates the potential sanitary risk associated with the presence of pathogenic bacteria in cultivated mussels in the two populous regions of southern Morocco, where shellfish production and maritime tourism are important to the local economy.

A study of pH effects on the bacterial surface physicochemical properties of Acinetobacter baumannii.

The first step in the biofilm formation is the bacterial attachment to solid surfaces, which is dependent on the bacteria cell surface physico-chemical properties. The purpose of this work was to analyze the effect of pH on the physicochemical cell surface properties of Acinetobacter baumannii by two different methods. The cell surface properties were evaluated using the microbial adhesion to solvents method (MATS) and contact angle measurements (CAM). MATS technique allowed us to enlighten that A. baumannii was hydrophilic at the different values of pH. It was found that at a desired pH of 6.5, the strain presents a maximum and stable value of electron-donor characteristic, while the electron acceptor character increased as the pH increased. Regardless of the methods employed, the obtained results using MATS and CAM confirmed the influence of the pH on the surface physicochemical properties of A. baumannii. The cell surface electron-donor and electron-acceptor character at pH 6.5 was found to be quite similar using both methods.

Environmental Scanning Electron Microscopy characterization of the adhesion of conidia from Penicillium expansum to cedar wood substrata at different pH values.

Initial microbial adhesion to surfaces is a complicated process that is affected by a number of factors. An important property of a solution that may influence adhesion is pH. The surface properties of the cedar wood were characterized by the sessile drop technique. Moreover, the interfacial free energy of surface adhesion to the cedar wood was determined under pH values (2, 3, 5, 7, 9 and 11). The results showed that cedar wood examined at different pH levels could be considered hydrophobic ranged from Giwi = -13.1 mJ/m(2) to Giwi = -75 mJ/m(2). We noted that the electron-donor character of cedar wood was important at both basic and limit acidic conditions (pH 11 and pH 3) and it decreased at intermediate pH (pH 5). The cedar wood substratum presents a weak electron acceptor under various pH's. In addition, the adhesion of conidia from Penicilllium expansum to the cedar wood surfaces at different pH values (2, 3, 5, 7, 9 and 11) was investigated using Environmental Scanning Electron Microscopy and image analysis was assessed with the Mathlab(®) program. The data analysis showed that the conidia from P. expansum were strongly influenced by the pH. The maximum adhesion occurs in the pH 11 and pH 3 and decreased to 24% at pH 5.

Evaluation of the relative cell surface charge by using microbial adhesion to hydrocarbon.

A simple and rapid method, Microbial adhesion to hexadecane, for estimating the cell surface charge is proposed. This method is based on the determination of cell affinity to hexadecane at low ionic strength and at high ionic strength. The difference between these two affinities can provide the relative cell surface charge. The application of this method for Staphylococcus aureus and Escherichia coli show that the profile of surface charge evolution as a function ofpH was similar to these obtained by microelectrophoresis method.

Comparison of contact angle measurement and microbial adhesion to solvents for assaying electron donor-electron acceptor (acid-base) properties of bacterial surface.

The electron donor-electron acceptor (acid-base properties) of cell surfaces of a series of bacteria were determined by two methods, namely, Microbial Adhesion to Solvents (MATS) and Contact Angle Measurements (CAM) combined with equation of Van Oss. The efficiency of these two methods was then compared. Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis ILP 142B, Staphylococcus aureus ATCC 25923 and four Escherichia coli strains including HB101, AL52, O128B12 and ATCC 25922, acid-base properties were examined under the two different conditions mentioned above. The results showed that the correlation between acid-base properties determined by MATS and CAM was very weak. We have also found that when the microbial cell surface was electron donor by CAM method, similar result was found by MATS, but the reverse was not always true. In contrast, a good correlation between the two methods was obtained when the four E. coli strains were examined.

The relation between Escherichia coli surface functional groups' composition and their physicochemical properties

Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.

Características de superfície de Escherichia coli, tais como hidrofobicidade, mobilidade eletroforética e composição dos grupos funcionais de superfície, foram estudadas. Essas características foram determinadas por medidas de angulo de contato com água, microeletroforese e espectroscopia fotoeletrônica de raio-X (XPS), respectivamente. A relação entre as propriedades fisicoquímicas e a composição dos grupos funcionais foi também examinada. Aparentemente, a mobilidade eletroforética em pH 7 é controlada na superfície celular pelos grupos funcionais (O=C). A hidrofilicidade da superfície celular estava associada com altos níveis dos grupos funcionais [C-(O.N)] e [OH-(C-O-C)], enquanto a hidrofobicidade estava associada com os grupos funcionais [C-(C,H)].

The relation between Escherichia coli surface functional groups' composition and their physicochemical properties.

Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH- (C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.

Association between plasmid carrying an expanded-spectrum cephalosporin resistance and biofilm formation in Escherichia coli.

The formation of biofilm is a universal bacterial survival strategy. Biofilms occur on inert and living support in the natural environment and in industrial installations. This microenvironment leads to the horizontal transfer of genetic material between bacteria by physical contact. In order to evaluate the relationship between biofilm-forming capabilities, surface characteristics and plasmid content we purified from Salmonella a plasmid conferring resistance to cephalosporin and transferred it by electroporation to E.coli DH10B originally unable to form biofilm in inert surface. We demonstrated the association between a plasmid conferring resistance to expanded-spectrum cephalosporin and biofilm formation. We also noted that this plasmid influences the cell surface properties and cell motility.