Environmental drivers of Euphorbia resinifera seed germination and seedling establishment for conservation purpose.

Euphorbia resinifera O. Berg is a prickly, leafless and succulent, Moroccan endemic shrub. Field data indicate that the plant faces many challenges related to its natural regeneration and its gradual decline that can lead to a probability of extinction, at least in some areas. Successful seed germination and survival of E. resinifera seedlings during the dry period is one of the main obstacles encountered in establishing natural seedlings. With this in mind, 3080 seeds of two morphotypes of E. resinifera (M1 and M2) were harvested in the Atlas of Beni Mellal to study their germinative potential and determine suitable conditions for growth and development of the seedlings. In the laboratory, five temperatures (10 °, 15 °C, 18 °C, 25 °C, and 35 °C) and two photoperiods (12 h light/12 h dark and 24 h dark) were tested. Whereas in field research, two factors were considered: the availability of water and the type of substrate (clay, peat, and limestone). Results show a maximum germination rate of around 52% for M2 at 15 °C and 48% for M1 at 18 °C. The Monitoring of plant seedling establishment and growth revealed a high vulnerability to prolonged periods of drought. However, consolidated soil is more conducive to seedling establishment. For this species, it is therefore essential to conserve the habitat within the karst geosystem. Furthermore, the variability of this species' morphotypes and their growth form architecture shows a tendency to favor the dwarf, cushion-shaped morphotype, which is the most widespread in the study area.

Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine.

A novel actinomycete, strain X4(T), was isolated from a phosphate mine in Youssoufia, 100 km north of Marrakesh, Morocco. The taxonomic status of this strain was evaluated by a polyphasic approach. Strain X4(T) had white aerial mycelium with Rectiflexibiles spore chains bearing smooth-surfaced spores and did not produce diffusible pigments. Chemotaxonomic analysis showed that the cell wall of strain X4(T) contained LL-diaminopimelic acid and glycine. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence indicated that strain X4(T) belongs to the Group I streptomycetes, branching off next to Streptomyces ramulosus NRRL B-2714(T) and Streptomyces kasugaensis M338-M1(T). DNA-DNA relatedness and phenotypic data enabled strain X4(T) to be distinguished from the phylogenetically most closely related type strains. It is therefore proposed that strain X4(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces youssoufiensis sp. nov. is proposed; the type strain is X4(T) ( = CCMM B709(T)  = DSM 41920(T)).