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1.
PLoS One ; 18(8): e0290173, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37585425

RESUMO

Arthropods account for a large proportion of animal biomass and diversity in terrestrial systems, making them crucial organisms in our environments. However, still too little is known about the highly abundant and megadiverse groups that often make up the bulk of collected samples, especially in the tropics. With molecular identification techniques ever more evolving, analysis of arthropod communities has accelerated. In our study, which was conducted within the Global Malaise trap Program (GMP) framework, we operated two closely placed Malaise traps in Padang, Sumatra, for three months. We analyzed the samples by DNA barcoding and sequenced a total of more than 70,000 insect specimens. For sequence clustering, we applied three different delimitation techniques, namely RESL, ASAP, and SpeciesIdentifier, which gave similar results. Despite our (very) limited sampling in time and space, our efforts recovered more than 10,000 BINs, of which the majority are associated with "dark taxa". Further analysis indicates a drastic undersampling of both sampling sites, meaning that the true arthropod diversity at our sampling sites is even higher. Regardless of the close proximity of both Malaise traps (< 360 m), we discovered significantly distinct communities.


Assuntos
Artrópodes , Biodiversidade , Animais , Código de Barras de DNA Taxonômico/métodos , Artrópodes/genética , DNA/genética , Biomassa
2.
Biodivers Data J ; 11: e104942, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37448693

RESUMO

Launched in 2015, the large-scale initiative Indonesian Biodiversity Discovery and Information System (IndoBioSys) is a multidisciplinary German-Indonesian collaboration with the main goal of establishing a standardised framework for species discovery and all associated steps. One aspect of the project includes the application of DNA barcoding for species identification and biodiversity assessments. In this framework, we conducted a large-scale assessment of the insect fauna of the Mount Halimun-Salak National Park which is one of the largest tropical rain-forest ecosystems left in West Java. In this study, we present the results of processing 5,034 specimens of Phoridae (scuttle flies) via DNA barcoding. Despite limited sequencing success, we obtained more than 500 clusters using different algorithms (RESL, ASAP, SpeciesIdentifier). Moreover, Chao statistics indicated that we drastically undersampled all trap sites, implying that the true diversity of Phoridae is, in fact, much higher. With this data release, we hope to shed some light on the hidden diversity of this megadiverse group of flies.

3.
Zookeys ; 897: 29-47, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31857786

RESUMO

The Indonesian species of the family Signiphoridae (Hymenoptera, Chalcidoidea) are revised. Three species of Chartocerus are described as new (Chartocerus kartiniae Polaszek & Schmidt, sp. nov., C. sumatrensis Schmidt & Polaszek, sp. nov., and C. javensis Schmidt & Ubaidillah, sp. nov.) and four species of Signiphora, viz., S. bennetti Woolley & Dal Molin, S. flavella Girault, S. perpauca Girault and S. bifasciata Ashmead, are diagnosed.

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