[Effect of 1,5-benzodiazepine derivatives on the electrical activity of neurons of the Helix albescens Rossm].

Using a standard technique for intracellular recording of potentials, we investigated the influence of two new derivatives of 1,5-benzodiazepine-2-one on the electrical activity of neurons of the Helix albescens Rossm. These substances possess neurotropic properties, the effects were concentration dependent in the concentration range 10(-6) M -10(-2) and did not depend on the type of neurons. We defined the threshold, optimum and toxic concentrations for the compounds investigared. Analysis of the total ion current showed that the compounds reduce the rate of inward Na+- and Ca2+ currents and of outward K+-currents.

[Effect of benzimidazole and its derivatives on electrical activity of Helix albescens Rossm neurons and behavior of rats].

We studied concentration dependency and the mechanisms of the effect of bezimidazole and its new derivatives on electrical processes in Helix albescens Rossm. neurons. These compounds appeared to have neurotropic properties dependent on their chemical structure and the types of neurons. We determined the threshold, optimal and toxic concentration values of the tested substances. Benzimidazole and 2-trifluoromethylbenzimidazole at concentrations of 10(-3) and 10(-2) M elicit both excitatory and inhibitory postsynaptic potentials on soma membrane of some pacemaker and non-pacemaker neurons and even cause a strong depolarization. The membrane of pacemaker neurons (namely, neuron RPa2) was found to have at least two spatially separated trigger sites with different trigger mechanism of action potential. After single intraperitoneal injection (50 mg/kg) of the substance solution, the tested compounds based on the discovered effects were separated into two groups. The first group inhibits locomotion and psychoemotional state of rats, the second group elicits the opposite effect. We found that at 100 and 150 mg/kg all tested substances inhibit the animal's activity.