RESUMO
The aim of the present study was to investigate the effect of miR133b on atherosclerosis (AS). A mouse model of AS (AS group) was established, and serum levels of total cholesterol, triglyceride, highdensity lipoprotein cholesterol and lowdensity lipoprotein (LDL) cholesterol were detected. The thoracic aorta tissues were subjected to hematoxylin and eosin staining for pathological examination. Mice were intravenously injected with microRNA (miR)133b mimics (the miR133b mimic + AS group) and miR133b mimics negative control (the miR133b NC + AS group). Normal mice were named the Sham group. Vascular reconstruction parameters, the Collagen/Vascular Area Ratio (CA/CVA) and serum inflammatory factors of mice in each group were detected. mRNA expression was measured by reverse transcriptionquantitative PCR and protein expression was determined by western blot analysis. An in vitro model of AS was induced in vascular smooth muscle cells (VSMCs) using oxidized (ox)LDL. CCK8 and wound healing assays were used to detect cell proliferation and migration. Compared with the Sham group, mice of the AS group, the AS + miR133b NC group and the AS + miR133b mimic group had higher intima thickness (IT), tumor necrosis factor (TNF)α and monocyte chemoattractant protein (MCP)1 levels, as well as increased Notch1 and Jagged1 expression; and they had lower medial thickness (MT), CA/CVA ratio and Notch3 expression (all P<0.05). In addition, miR133b mimic promoted the proliferation and migration, upregulated Notch1 and Jagged1, and downregulated Notch3 in oxLDLinduced VSMCs. Taken together, miR133b aggravates AS by activating the Notch signaling pathway, which could serve as a potential target for the treatment of AS.
Assuntos
Aterosclerose/patologia , MicroRNAs/farmacologia , Miócitos de Músculo Liso , Receptor Notch1/metabolismo , Animais , Linhagem Celular , Movimento Celular , Proliferação de Células , Proteína Jagged-1/metabolismo , Masculino , Camundongos , Camundongos Knockout para ApoE , Miócitos de Músculo Liso/metabolismo , Miócitos de Músculo Liso/patologia , Receptor Notch3/metabolismoRESUMO
Given the proliferation in studies investigating green coffee bean extract (GCBE) supplementation, the purpose of this study was to determine the efficacy and effectiveness of GCBE supplementation on indices of blood pressure. The literature search was performed in four databases, namely, PubMed/Medline, Scopus, the Cochrane Library, and Google Scholar, to identify clinical trials that examined the effects of green coffee supplements on systolic blood pressure (SBP) and diastolic blood pressure (DBP) up to February 2019. Mean change and standard deviation (SD) of the outcome measures were used to estimate the mean difference between the intervention group and the control group at follow-up. Nine studies reported SBP and DBP as an outcome measure. Results revealed significant reduction in SBP (weighted mean difference: -3.093 mmHg, 95% confidence interval [CI]: -3.914, -2.273; I2 = 0.0%) and DBP (-2.170 mmHg, 95% CI: -2.749, -1.590; I2 = 46.5%) after green coffee supplementation with low heterogeneity among the studies. In addition, in subgroup analysis, a significant reduction in SBP and DBP in studies with hypertensive patients, green coffee dosage <400 mg, and administered for 4 weeks was identified. The results of the current meta-analysis study support the use of GCBE supplementation for the improvement of blood pressure indices, with subgroup analysis highlighting improvements in hypertensive patients.