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1.
Waste Manag Res ; : 734242X241241602, 2024 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-38563513

RESUMO

The development of the solar market has been fast in the past decades, and the number of photovoltaic module installations is large. The photovoltaic modules have a lifetime of about 25 years and need recovery after that. The aluminium-back surface field (Al-BSF) module is the first kind of large-scale installed module and will come to its end of life in the next few years. The recycling of silicon material in the Al-BSF module is investigated in this work. The components of the module are separated, and the silicon material in the module is collected and then purified by (aluminium-silicon) Al-Si solvent refining for reuse. It is found that Al-Si solvent refining removed key impurity elements, namely boron and phosphorus, in the collected silicon. Kinetics has a great effect on boron and phosphorus removal, and boron and phosphorus contents in purified silicon decrease with decreasing cooling rate. The boron and phosphorus contents in silicon are lowered to 0.28 and 0.03 ppmw, respectively, after two times of Al-Si solvent refining with the cooling rate of 5.55 * 10-4 K second-1, and it meets the requirement of solar-grade silicon.

2.
Neuropharmacology ; 133: 155-162, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29378210

RESUMO

Maternal sleep deprivation (MSD) has been suggested to be associated with increased frequency of neurodevelopmental disorders in offspring in both humans and animal models. However, the underlying cellular and molecular mechanism is still unclear. We have recently reported that MSD at different stages of pregnancy impairs the emotional and cognitive functions, and suppresses hippocampal CA1 long-term potentiation (LTP) in the offspring rats. Here, we report that the MSD induced LTP impairment at the CA1 hippocampus of the offspring rats is associated with increased long-term depression (LTD) and reduced expression of postsynaptic GluA2-containing α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid receptors (AMPARs). Importantly, we found that inhibition of AMPAR endocytosis by a synthetic peptide Tat-GluA23Y (3 µmol/kg, i.p.) not only increased level of AMPARs and reduced LTD, but also restored LTP. Moreover, treatment with Tat-GluA23Y peptide markedly alleviated the MSD-induced impairments of spatial learning and memory; and decreased depressive- and anxiety-like behaviors in the offspring. Together, our findings suggest that the MSD-induced postsynaptic AMPAR endocytosis causally contributes to the impairments of hippocampal synaptic plasticity, thereby disrupting the emotional and cognitive functions in the offspring.


Assuntos
Endocitose/fisiologia , Transtornos do Neurodesenvolvimento/etiologia , Transtornos do Neurodesenvolvimento/patologia , Plasticidade Neuronal/fisiologia , Efeitos Tardios da Exposição Pré-Natal/fisiopatologia , Receptores de AMPA/metabolismo , Privação do Sono/complicações , Animais , Animais Recém-Nascidos , Proteína 4 Homóloga a Disks-Large/metabolismo , Reação de Fuga/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Feminino , Hipocampo/patologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Aprendizagem em Labirinto/fisiologia , Gravidez , Ratos , Ratos Sprague-Dawley , Tempo de Reação/efeitos dos fármacos , Natação/psicologia
3.
Behav Brain Res ; 339: 66-72, 2018 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-29162383

RESUMO

Our previous study has shown that pentobarbital causes memory deficits and impairs hippocampal synaptic plasticity. The Tat-GluA23Y peptide (GluA23Y) prevents activity-dependent α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) endocytosis. It enables early-phase long-term potentiation (LTP) to proceed to late-phase LTP allowing short-term memory to convert to long-term memory. The purpose of this study is to explore the potential effects of GluA23Y on pentobarbital-induced memory deficits through behavioral and electrophysiological paradigms. We found that in vivo intrahippocampal infusion of GluA23Y (100µM, 1µl per hippocampus) 30min prior to pentobarbital administration (8mM, 1µl per hippocampus) significantly rescued the pentobarbital-induced deficit of memory retrieval in rats during the Morris water maze test. Pre-incubation of GluA23Y (10µM) partially rescued bath application of pentobarbital-induced synaptic transmission of the CA3-CA1 pathway in hippocampal slices. More importantly, GluA23Y selectively upregulated the synaptic GluA2 expression that was suppressed by pentobarbital. Together, these results suggest that inhibition of GluA2-containing AMPAR endocytosis by GluA23Y increases the pentobarbital-suppressed basal synaptic transmission by upregulating the synaptic GluA2, and then subsequently alleviates spatial memory deficits. Therefore, inhibition of AMPAR endocytosis may be a potential therapeutic way to treat memory disorders caused by anesthetics.


Assuntos
Hipocampo/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Plasticidade Neuronal/efeitos dos fármacos , Pentobarbital/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Animais , Depressão/induzido quimicamente , Endocitose/efeitos dos fármacos , Hipocampo/metabolismo , Masculino , Transtornos da Memória/induzido quimicamente , Memória de Longo Prazo/efeitos dos fármacos , Ratos Sprague-Dawley
4.
J Chem Phys ; 147(5): 054302, 2017 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-28789540

RESUMO

We calculate the rovibrational bound levels with total angular momentum J = 0, 1 of 40Ar284Kr trimer using the slow variable discretization method combined with the finite-element method-discrete variable representation basis. The statistical distributions of the rovibrational levels for JΠ=0e, 1e, and 1o symmetries are presented and the effects of the Axilrod-Teller potential term are considered. For the 0e and 1e symmetries, the Axilrod-Teller term makes the spectra become fully chaotic. However, for the 1o symmetry, statistical properties depend mainly on the coupling between K = 0 and K = 1 and the Axilrod-Teller term has a small effect.

5.
Sci Total Environ ; 607-608: 541-548, 2017 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-28704677

RESUMO

As one of the most reactive species, hydrated electron (eaq-) is promising for reductive decomposition of recalcitrant organic pollutants, such as perfluorooctane sulfonate (PFOS). In this study, PFOS decomposition using a vacuum ultraviolet (VUV)/sulfite system was systematically investigated in comparison with sole VUV and ultraviolet (UV)/sulfite systems. A fast and nearly complete (97.3%) PFOS decomposition was observed within 4h from its initial concentration of 37.2µM in the VUV/sulfite system. The observed rate constant (kobs) for PFOS decomposition in the studied system was 0.87±0.0060h-1, which was nearly 7.5 and 2 folds faster than that in sole VUV and UV/sulfite systems, respectively. Compared to previously studied UV/sulfite system, VUV/sulfite system enhanced PFOS decomposition in both weak acidic and alkaline pH conditions. In weak acidic condition (pH6.0), PFOS predominantly decomposed via direct VUV photolysis, whereas in alkaline condition (pH>9.0), PFOS decomposition was mainly induced by eaq- generated from both sulfite and VUV photolytic reactions. At a fixed initial solution pH (pH10.0), PFOS decomposition kinetics showed a positive linear dependence with sulfite dosage. The co-presence of humic acid (HA) and NO3- obviously suppressed PFOS decomposition, whereas HCO3- showed marginal inhibition. A few amount of short chain perfluorocarboxylic acids (PFCAs) were detected in PFOS decomposition process, and a high defluorination efficiency (75.4%) was achieved. These results suggested most fluorine atoms in PFOS molecule ultimately mineralized into fluoride ions, and the mechanisms for PFOS decomposition in the VUV/sulfite system were proposed.

6.
J Alzheimers Dis ; 59(2): 683-694, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28671132

RESUMO

Alzheimer's disease (AD) is the most common cause of progressive cognitive impairment in the aged. The aggregation of the amyloid ß-protein (Aß) is a hallmark of AD and is linked to synapse loss and cognitive impairment. Capsaicin, a specific agonist of the transient receptor potential vanilloid 1 (TRPV1), has been proven to ameliorate stress-induced AD-like pathological and cognitive impairments, but it is unclear whether TRPV1 activation can affect cognitive and synaptic functions in Aß-induced mouse model of AD. In this study, we investigated the effects of TRPV1 activation on spatial memory and synaptic plasticity in mice treated with Aß. To induce AD-like pathological and cognitive impairments, adult C57Bl/6 mice were microinjected with Aß42 (100 µM, 2.5 µl/mouse, i.c.v.). Two weeks after Aß42 microinjection, spatial learning and memory as well as hippocampal long-term potentiation (LTP) were examined. The results showed that Aß42 microinjection significantly impaired spatial learning and memory in the Morris water maze and novel object recognition tests compared with controls. These behavioral changes were accompanied by synapse loss and impaired LTP in the CA1 area of hippocampus. More importantly, daily capsaicin (1 mg/kg, i.p.) treatment throughout the experiment dramatically improved spatial learning and memory and synaptic function, as reflected by enhanced hippocampal LTP and reduced synapse loss, whereas the TRPV1 antagonist capsazepine (1 mg/kg, i.p.) treatment had no effects on cognitive and synaptic function in Aß42-treated mice. These results indicate that TRPV1 activation by capsaicin rescues cognitive deficit in the Aß42-induced mouse model of AD both structurely and functionally.


Assuntos
Peptídeos beta-Amiloides/toxicidade , Encéfalo/patologia , Capsaicina/uso terapêutico , Disfunção Cognitiva , Fragmentos de Peptídeos/toxicidade , Fármacos do Sistema Sensorial/uso terapêutico , Sinapses/efeitos dos fármacos , Análise de Variância , Animais , Disfunção Cognitiva/induzido quimicamente , Disfunção Cognitiva/tratamento farmacológico , Disfunção Cognitiva/patologia , Modelos Animais de Doenças , Proteína 4 Homóloga a Disks-Large/metabolismo , Estimulação Elétrica , Comportamento Exploratório/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Eletrônica de Transmissão , Reconhecimento Psicológico/efeitos dos fármacos , Sinapses/patologia , Sinapses/ultraestrutura , Sinaptofisina/metabolismo , Fatores de Tempo
7.
Proc Natl Acad Sci U S A ; 114(9): 2401-2406, 2017 02 28.
Artigo em Inglês | MEDLINE | ID: mdl-28223480

RESUMO

Migraine is characterized by severe headaches that can be preceded by an aura likely caused by cortical spreading depression (SD). The antiepileptic pregabalin (Lyrica) shows clinical promise for migraine therapy, although its efficacy and mechanism of action are unclear. As detected by diffusion-weighted MRI (DW-MRI) in wild-type (WT) mice, the acute systemic administration of pregabalin increased the threshold for SD initiation in vivo. In familial hemiplegic migraine type 1 mutant mice expressing human mutations (R192Q and S218L) in the CaV2.1 (P/Q-type) calcium channel subunit, pregabalin slowed the speed of SD propagation in vivo. Acute systemic administration of pregabalin in vivo also selectively prevented the migration of SD into subcortical striatal and hippocampal regions in the R192Q strain that exhibits a milder phenotype and gain of CaV2.1 channel function. At the cellular level, pregabalin inhibited glutamatergic synaptic transmission differentially in WT, R192Q, and S218L mice. The study describes a DW-MRI analysis method for tracking the progression of SD and provides support and a mechanism of action for pregabalin as a possible effective therapy in the treatment of migraine.


Assuntos
Analgésicos/farmacologia , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio Tipo N/genética , Ataxia Cerebelar/tratamento farmacológico , Depressão Alastrante da Atividade Elétrica Cortical/efeitos dos fármacos , Transtornos de Enxaqueca/tratamento farmacológico , Enxaqueca com Aura/tratamento farmacológico , Pregabalina/farmacologia , Animais , Encéfalo/diagnóstico por imagem , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Canais de Cálcio Tipo N/metabolismo , Ataxia Cerebelar/diagnóstico por imagem , Ataxia Cerebelar/metabolismo , Ataxia Cerebelar/patologia , Imagem de Difusão por Ressonância Magnética , Modelos Animais de Doenças , Expressão Gênica , Humanos , Camundongos , Camundongos Transgênicos , Transtornos de Enxaqueca/diagnóstico por imagem , Transtornos de Enxaqueca/metabolismo , Transtornos de Enxaqueca/patologia , Enxaqueca com Aura/diagnóstico por imagem , Enxaqueca com Aura/metabolismo , Enxaqueca com Aura/patologia , Mutação , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Neurônios/patologia , Transmissão Sináptica
8.
Huan Jing Ke Xue ; 38(4): 1477-1482, 2017 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965149

RESUMO

The objective of this experiment was to investigate the influence of SO32-dosage, pH value, initial perfluorooctane sulfonate(PFOS)concentration as well as coexisting substances on the degradation and defluorination of PFOS by VUV-SO32- process. The results indicated that the increase of SO32- dosage could lead to rise in the concentration of active species hydrated electron(eaq-) and thus enhance the degradation and defluorination of PFOS. As the concentration of SO32- increased from 1 mmol·L-1 to 20 mmol·L-1, the degradation and defluorination rates of PFOS increased from 45% and 40% to 97% and 63%, respectively. The degradation and defluorination of PFOS were also enhanced with the increment of the solution pH values, and the defluorination was more sensitive to the pH values. In addition, more PFOS was degraded with the increase of initial PFOS mass concentration, although the degradation and defluorination rates of PFOS were reduced. When the initial PFOS mass concentration changed from 1 mg·L-1 to 50 mg·L-1, the degradation amount of PFOS after four hrs increased by about 50 times, probably due to the higher utilization proportion of eaq- at high pollutants concentration. Last but not least, the influence of co-existing substances, Cl- and HCO3-, on PFOS degradation could be neglected, whereas their effects on defluorination were observed. Defluorination of PFOS was enhanced with the increased Cl- concentration, however, increased first and then decreased with the increment of HCO3- concentration. It was also found that the presence of humic acid(HA) lowered degradation as well as defluorination of PFOS owing to the blockage of effective UV light and trapping of active species for photochemical reaction.

9.
Front Pharmacol ; 7: 318, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27679577

RESUMO

Atopic dermatitis (AD) is a common chronic inflammatory skin disorder, and patients with AD suffer from severe psychological stress, which markedly increases the prevalence rate of depression and anxiety disorders in later life. Fluoxetine, a selective serotonin reuptake inhibitor, has recently been reported to exert anti-inflammatory and immunosuppressive effects. However, it is unclear whether fluoxetine is effective in the treatment of AD through reducing psychological stress and inflammatory reaction. Here, we reported that a BALB/c mouse model of AD was induced by application of 2,4-dinitrochlorobenzene (DNCB) onto hairless dorsal skin. Chronic fluoxetine treatment (10 mg/kg per day, i.p.) significantly attenuated AD-like symptoms, as reflected by a dramatic decrease in scratching bouts, as well as a decrease in anxiety- and depressive-like behaviors. Furthermore, these behavioral changes were accompanied by a significant decrease in epidermal thickness, the number of mast cells in skin tissue, mRNA levels of interleukin-4 (IL-4) and IL-13 in the spleen, as well as serum immunoglobulin E (IgE) in the DNCB-treated mice by treatment with fluoxetine. Taken together, these results indicate that fluoxetine may suppress psychological stress and inflammatory response during AD development, and subsequently ameliorate AD symptoms, suggesting that fluoxetine may be a potential therapeutic agent against AD in clinic.

10.
J Chem Phys ; 145(3): 034304, 2016 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-27448884

RESUMO

The ground state vibrational energy and spatial features of (4)He-(4)He-Li(+) and its triatomic isotopic complexes are studied using the slow variable discretization (SVD) method in the hyperspherical coordinates for the zero total angular momentum. Our results show that the dominant structure of the system is an isosceles triangle with the shorter side associated with the two Li(+)-He distances using the sum-of-potential approximation. Corrections caused by the induced dipole-induced dipole interactions on the He atoms are also investigated. The effects are seen to be small and have a minor influence on the binding energy and the structure of present system. The results are also compared with the full ab initio calculations including all the three-body interactions and information of three-body corrections is obtained.

11.
Nanotechnology ; 27(31): 315105, 2016 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-27346852

RESUMO

A safe and efficient nanocomposite hydrogel for colon cancer drug delivery was synthesized using pH-sensitive and biocompatible graphene oxide (GO) containing azoaromatic crosslinks as well as poly (vinyl alcohol) (PVA) (GO-N=N-GO/PVA composite hydrogels). Curcumin (CUR), an anti-cancer drug, was encapsulated successfully into the hydrogel through a freezing and thawing process. Fourier transform infrared spectroscopy, scanning electron microscopy and Raman spectroscopy were performed to confirm the formation and morphological properties of the nanocomposite hydrogel. The hydrogels exhibited good swelling properties in a pH-sensitive manner. Drug release studies under conditions mimicking stomach to colon transit have shown that the drug was protected from being released completely into the physiological environment of the stomach and small intestine. In vivo imaging analysis, pharmacokinetics and a distribution of the gastrointestinal tract experiment were systematically studied and evaluated as colon-specific drug delivery systems. All the results demonstrated that GO-N=N-GO/PVA composite hydrogels could protect CUR well while passing through the stomach and small intestine to the proximal colon, and enhance the colon-targeting ability and residence time in the colon site. Therefore, CUR loaded GO-N=N-GO/PVA composite hydrogels might potentially provide a theoretical basis for the treatment of colon cancer with high efficiency and low toxicity.

12.
CNS Neurosci Ther ; 22(7): 617-24, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27079181

RESUMO

BACKGROUND: Parkinson disease (PD) is a neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra (SN) and diminished dopamine content in the striatum, which is at least partly associated with α-synuclein protein overexpression in these neurons. Recent reports show that 7,8-dihydroxyflavone (DHF), a TrkB agonist, has beneficial effects in animal model of PD. However, it is unclear whether the therapeutic effects of DHF are associated with the expression of α-synuclein. AIMS: In this study, we investigated the protective effects of DHF on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced deficit of motor functions, the loss of dopaminergic neurons and the expression of α-synuclein as well as antioxidative activity in the C57BL/6 mice. RESULTS: Mice were treated with MPTP (30 mg/kg, i.p.) once a day for 5 days to induce dopaminergic neuron death in the SN. DHF (5 mg/kg, i.p.) was administrated once a day from the first day of MPTP injection until 9 days after the last injection of MPTP. Behavioral tests showed that DHF succeeded in ameliorating the impaired motor functions in the MPTP-treated mice. The immunohistochemical assay showed that the amelioration of motor function was accompanied by a reduction in the loss of dopaminergic neurons in the SN and striatum. Western blot analyses showed that DHF prevented the inactivation of TrkB and suppressed α-synuclein overexpression in the SN and striatum following MPTP treatment. Antioxidative activity detection revealed that DHF prevented MPTP-induced reduction in glutathione and total superoxide dismutase activity in the SN and striatum. CONCLUSION: Taken together, these results indicate that DHF treatment may suppress the accumulation of α-synuclein and oxidative stress via activating TrkB and subsequently block the loss of dopaminergic neurons in the SN and striatum, thereby ameliorating MPTP-induced motor deficits in the C57BL/6 mice.


Assuntos
Antiparkinsonianos/uso terapêutico , Flavonas/uso terapêutico , Regulação da Expressão Gênica/efeitos dos fármacos , Intoxicação por MPTP , Estresse Oxidativo/efeitos dos fármacos , alfa-Sinucleína/metabolismo , Análise de Variância , Animais , Morte Celular/efeitos dos fármacos , Modelos Animais de Doenças , Intoxicação por MPTP/tratamento farmacológico , Intoxicação por MPTP/metabolismo , Intoxicação por MPTP/fisiopatologia , Camundongos , Camundongos Endogâmicos C57BL , Movimento/efeitos dos fármacos , Desempenho Psicomotor/efeitos dos fármacos , Receptor trkA/metabolismo , Teste de Desempenho do Rota-Rod , Tirosina 3-Mono-Oxigenase/metabolismo
13.
Mol Brain ; 9: 17, 2016 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-26876533

RESUMO

BACKGROUND: Sleep deprivation during pregnancy is a serious public health problem as it can affect the health of pregnant women and newborns. However, it is not well studied whether sleep deprivation at different stages of pregnancy has similar effects on emotional and cognitive functions of the offspring, and if so, the potential cellular mechanisms also remain poorly understood. METHODS: In the present study, the pregnant rats were subjected to sleep deprivation for 6 h per day by gentle handling during the first (gestational days 1-7), second (gestational days 8-14) and third trimester (gestational days 15-21) of pregnancy, respectively. The emotional and cognitive functions as well as hippocampal long-term potentiation (LTP) were tested in the offspring rats (postnatal days 42-56). RESULTS: The offspring displayed impaired hippocampal-dependent spatial learning and memory, and increased depressive- and anxiety-like behaviors. Quantification of BrdU-positive cells revealed that adult hippocampal neurogenesis was significantly reduced compared to control. Electrophysiological recording showed that maternal sleep deprivation impaired hippocampal CA1 LTP and reduced basal synaptic transmission, as reflected by a decrease in the frequency and amplitude of miniature excitatory postsynaptic current in the hippocampal CA1 pyramidal neurons. CONCLUSIONS: Taken together, these results suggest that maternal sleep deprivation at different stages of pregnancy disrupts the emotional and cognitive functions of the offspring that might be attributable to the suppression of hippocampal LTP and basal synaptic transmission.


Assuntos
Cognição , Emoções , Hipocampo/fisiopatologia , Potenciação de Longa Duração/fisiologia , Privação do Sono/fisiopatologia , Animais , Animais Recém-Nascidos , Ansiedade/complicações , Ansiedade/fisiopatologia , Comportamento Animal , Proliferação de Células , Sobrevivência Celular , Depressão/complicações , Depressão/fisiopatologia , Potenciais Pós-Sinápticos Excitadores , Feminino , Masculino , Aprendizagem em Labirinto , Memória , Neurogênese , Neurônios/patologia , Gravidez , Ratos Sprague-Dawley , Privação do Sono/complicações , Natação
14.
J Pain ; 17(2): 181-9, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26521174

RESUMO

UNLABELLED: Itch and pain are unpleasant sensations that distress many patients with disease. However, most studies have focused on the neural mechanisms of pain, and much less effort has been devoted to itch. It has been reported that itch and pain might share a common pathway, and γ-aminobutyric acid type A (GABA(A)) receptors in the central nucleus of the amygdala (CeA) are involved in pain modulation. However, the contribution of GABAA receptors in the CeA to the modulation of itch remains poorly understood. Herein, we report that bilateral intra-CeA microinjection of a selective GABAA receptor agonist muscimol hydrochloride (Mus; 50 ng per side), but not a selective GABAA receptor antagonist bicuculline (Bic; 20 ng per side) or vehicle, showed significant analgesic effects, reflected by an increase in tail-flick latency and a decrease in allyl isothiocyanate (mustard oil)-evoked ipsilateral forelimb wipes. More importantly, rats subjected to intra-CeA infusion of Bic showed a significantly greater number of scratching bouts and time in acute and chronic pruritus animal models than control rats. Conversely, intra-CeA infusion of Mus in animal models dramatically decreased the number of scratching bouts and time compared with control rats. In addition, intra-CeA infusion of Bic or Mus at the current dose had no obvious effects on other behaviors including locomotor activity and spontaneous facial grooming in rats subjected to cheek microinjection of 5-hydroxytryptamine. Taken together, these results indicate that the GABA(A) receptor-mediated inhibitory system in the CeA is involved in itch modulation as well as is known in pain control. PERSPECTIVE: Itch, especially chronic itch, remains a challenge in clinic. Results of this study showed that the GABAA receptors in the CeA play an important role in itch modulation, which might help us to better understand the mechanisms of itch and subsequently develop novel mechanisms-based strategies to treat chronic itch in clinic.


Assuntos
Comportamento Animal/efeitos dos fármacos , Núcleo Central da Amígdala/metabolismo , Agonistas de Receptores de GABA-A/farmacologia , Antagonistas de Receptores de GABA-A/farmacologia , Dor/metabolismo , Prurido/metabolismo , Receptores de GABA-A/metabolismo , Animais , Bicuculina/administração & dosagem , Bicuculina/farmacologia , Modelos Animais de Doenças , Agonistas de Receptores de GABA-A/administração & dosagem , Antagonistas de Receptores de GABA-A/administração & dosagem , Masculino , Muscimol/administração & dosagem , Muscimol/farmacologia , Dor/tratamento farmacológico , Prurido/tratamento farmacológico , Ratos , Ratos Sprague-Dawley
15.
Sci Rep ; 5: 12667, 2015 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-26218751

RESUMO

A growing body of evidence has shown that chronic treatment with fluoxetine, a widely prescribed medication for treatment of depression, can affect synaptic plasticity in the adult central nervous system. However, it is not well understood whether acute fluoxetine influences synaptic plasticity, especially on hippocampal CA1 long-term depression (LTD), and if so, whether it subsequently impacts hippocampal-dependent spatial memory. Here, we reported that LTD facilitated by elevated-platform stress in hippocampal slices was completely prevented by fluoxetine administration (10 mg/kg, i.p.) 30 min before stress. The LTD was not, however, significantly inhibited by fluoxetine administration immediately after stress. Similarly, fluoxetine incubation (10 µM) during electrophysiological recordings also displayed no influence on the stress-facilitated LTD. In addition, behavioral results showed that a single fluoxetine treatment 30 min before but not after acute stress fully reversed the impairment of spatial memory retrieval in the Morris water maze paradigm. Taken together, these results suggest that acute fluoxetine treatment only before, but not after stress, can prevent hippocampal CA1 LTD and spatial memory retrieval impairment caused by behavioral stress in adult animals.


Assuntos
Fluoxetina/farmacologia , Depressão Sináptica de Longo Prazo/efeitos dos fármacos , Transtornos da Memória/prevenção & controle , Memória Espacial/efeitos dos fármacos , Estresse Psicológico/fisiopatologia , Análise de Variância , Animais , Antidepressivos de Segunda Geração/farmacologia , Região CA1 Hipocampal/efeitos dos fármacos , Região CA1 Hipocampal/fisiologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Aprendizagem em Labirinto/fisiologia , Transtornos da Memória/fisiopatologia , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/fisiologia , Ratos Sprague-Dawley , Memória Espacial/fisiologia , Fatores de Tempo
16.
Physiol Behav ; 149: 169-73, 2015 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-26056078

RESUMO

Reports of the effects of pentobarbital on learning and memory are contradictory. Some studies have not shown any interference with learning and memory, whereas others have shown that pentobarbital impairs memory and that these impairments can last for long periods. However, it is unclear whether acute local microinjections of pentobarbital affect learning and memory, and if so, the potential mechanisms are also unclear. Here, we reported that the intra-hippocampal infusion of pentobarbital (8.0mM, 1µl per side) significantly impaired hippocampus-dependent spatial learning and memory retrieval. Moreover, in vitro electrophysiological recordings revealed that these behavioral changes were accompanied by impaired hippocampal CA1 long-term potentiation (LTP) and suppressed neuronal excitability as reflected by a decrease in the number of action potentials (APs). These results suggest that acute pentobarbital application causes spatial learning and memory deficits that might be attributable to the suppression of synaptic plasticity and neuronal excitability.


Assuntos
Hipocampo/efeitos dos fármacos , Hipnóticos e Sedativos/toxicidade , Deficiências da Aprendizagem/induzido quimicamente , Potenciação de Longa Duração/efeitos dos fármacos , Transtornos da Memória/induzido quimicamente , Pentobarbital/toxicidade , Animais , Bicuculina/farmacologia , Biofísica , Relação Dose-Resposta a Droga , Estimulação Elétrica , Reação de Fuga/efeitos dos fármacos , Antagonistas de Receptores de GABA-A/farmacologia , Técnicas In Vitro , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Tempo de Reação/efeitos dos fármacos , Aprendizagem Espacial/efeitos dos fármacos , Fatores de Tempo
17.
Sci Rep ; 5: 9666, 2015 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-25942289

RESUMO

Addictive behavior is increasingly accepted as a drug-associated pathological memory in which the hippocampus is profoundly engaged. It has been well documented that adaptations of synaptic plasticity of excitatory transmission in the hippocampus may contribute to opioid addiction. However, it remains unknown whether and how adaptive changes of synaptic plasticity of inhibitory transmission in the hippocampus occurs during opioid abuse. Here, we reported that a single in vivo morphine exposure (SM) did not affect inhibitory long-term depression (I-LTD) in the hippocampus, compared with saline control; while repeated morphine exposure (RM) abolished this I-LTD. Interestingly, opioid withdrawal for 3-5 days after repeated (RMW), but not a single morphine exposure (SMW), largely enhanced I-LTD. More importantly, the I-LTD in single morphine treatment is dependent on presynaptic mechanism since it can be blocked by AM251, a selective cannabinoid receptor 1 antagonist. While the large I-LTD in RMW group is dependent on combinatorial presynaptic and postsynaptic mechanisms since it can be blocked by co-application of AM251 and L-type calcium channel blocker LaCl3. Thus, these results demonstrate that opioid use and withdrawal drive the dynamics of presynaptic and postsynaptic I-LTD expression in the hippocampus that may contribute to the persistent behavioral changes during opioid abuse.


Assuntos
Hipocampo/fisiopatologia , Depressão Sináptica de Longo Prazo , Dependência de Morfina/fisiopatologia , Inibição Neural , Síndrome de Abstinência a Substâncias/fisiopatologia , Transmissão Sináptica , Animais , Doença Crônica , Masculino , Ratos , Ratos Wistar
18.
Pharmacol Biochem Behav ; 133: 51-6, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25843400

RESUMO

It is well known that bidirectional glia-neuron interactions play important roles in the neurophysiological and neuropathological processes. It is reported that impairing glial functions with sodium fluoroacetate (FAC) impaired hippocampal long-term depression (LTD) and spatial memory retrieval. However, it remains unknown whether FAC impairs hippocampal long-term potentiation (LTP) and learning and/or memory, and if so, whether pharmacological treatment with exogenous d-serine can recuse the impairment. Here, we reported that systemic administration of FAC (3mg/kg, i.p.) before training resulted in dramatic impairments of spatial learning and memory in water maze and fear memory in contextual fear conditioning. Furthermore, the behavioral deficits were accompanied by impaired LTP induction in the hippocampal CA1 area of brain slices. More importantly, exogenous d-serine treatment succeeded in recusing the deficits of hippocampal LTP and learning and memory induced by FAC. Together, these results suggest that astrocytic d-serine may be essential for hippocampal synaptic plasticity and memory, and that alteration of its levels may be relevant to the induction and potentially treatment of psychiatric and neurological disorders.


Assuntos
Condicionamento Psicológico/efeitos dos fármacos , Medo/efeitos dos fármacos , Fluoracetatos/antagonistas & inibidores , Fluoracetatos/farmacologia , Hipocampo/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Serina/farmacologia , Animais , Masculino , Ratos
19.
J Clin Invest ; 125(1): 234-47, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25437879

RESUMO

Long-term potentiation (LTP) of synaptic strength between hippocampal neurons is associated with learning and memory, and LTP dysfunction is thought to underlie memory loss. LTP can be temporally and mechanistically classified into decaying (early-phase) LTP and nondecaying (late-phase) LTP. While the nondecaying nature of LTP is thought to depend on protein synthesis and contribute to memory maintenance, little is known about the mechanisms and roles of decaying LTP. Here, we demonstrated that inhibiting endocytosis of postsynaptic α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid receptors (AMPARs) prevents LTP decay, thereby converting it into nondecaying LTP. Conversely, restoration of AMPAR endocytosis by inhibiting protein kinase Mζ (PKMζ) converted nondecaying LTP into decaying LTP. Similarly, inhibition of AMPAR endocytosis prolonged memory retention in normal animals and reduced memory loss in a murine model of Alzheimer's disease. These results strongly suggest that an active process that involves AMPAR endocytosis mediates the decay of LTP and that inhibition of this process can prolong the longevity of LTP as well as memory under both physiological and pathological conditions.


Assuntos
Endocitose , Potenciação de Longa Duração , Transtornos da Memória/metabolismo , Receptores de AMPA/metabolismo , Doença de Alzheimer/metabolismo , Doença de Alzheimer/fisiopatologia , Doença de Alzheimer/psicologia , Animais , Região CA1 Hipocampal/fisiopatologia , Humanos , Masculino , Memória de Longo Prazo , Memória de Curto Prazo , Camundongos Transgênicos , Proteína Quinase C/genética , Proteína Quinase C/metabolismo , Transporte Proteico , Ratos Sprague-Dawley
20.
Front Behav Neurosci ; 8: 325, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25294992

RESUMO

Dysfunction of central serotonin (5-HT) system has been proposed to be one of the underlying mechanisms for anxiety and depression, and the association of diabetes mellitus and psychiatric disorders has been noticed by the high prevalence of anxiety/depression in patients with diabetes mellitus. This promoted us to examine these behaviors in central 5-HT-deficient mice and those also suffering with diabetes mellitus. Mice lacking either 5-HT or central serotonergic neurons were generated by conditional deletion of Tph2 or Lmx1b respectively. Simultaneous depletion of both central serotonergic neurons and pancreatic islet cells was achieved by administration of diphtheria toxin (DT) in Pet1-Cre;Rosa26-DT receptor (DTR) mice. The central 5-HT-deficient mice showed reduced anxiety-like behaviors as they spent more time in and entered more often into the light box in the light/dark box test compared with controls; similar results were observed in the elevated plus maze test. However, they displayed no differences in the immobility time of the forced swimming and tail suspension tests suggesting normal depression-like behaviors in central 5-HT-deficient mice. As expected, DT-treated Pet1-Cre;Rosa26-DTR mice lacking both central serotonergic neurons and pancreatic islet endocrine cells exhibited several classic diabetic symptoms. Interestingly, they displayed increased anxiety-like behaviors but reduced immobility time in the forced swimming and tail suspension tests. Furthermore, the hippocampal neurogenesis was dramatically enhanced in these mice. These results suggest that the deficiency of central 5-HT may not be sufficient to induce anxiety/depression-like behaviors in mice, and the enhanced hippocampal neurogenesis may contribute to the altered depression-like behaviors in the 5-HT-deficient mice with diabetes. Our current investigation provides understanding the relationship between diabetes mellitus and psychiatric disorders.

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