Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Viruses ; 14(2)2022 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-35215950

RESUMO

Encephalomyocarditis virus can cause myocarditis and encephalitis in pigs and other mammals, thus posing a potential threat to public health safety. The 2A protein is an important virulence factor of EMCV. Previous studies have shown that the 2A protein may be related to the inhibition of apoptosis by virus, but its specific molecular mechanism is not clear. In this study, the 2A protein was expressed in Escherichia coli in order to find interacting cell proteins. A pull down assay, coupled with mass spectrometry, revealed that the 2A protein possibly interacted with annexin A2. Co-immunoprecipitation assays and confocal imaging analysis further demonstrated that the 2A protein interacted with annexin A2 in cells. In reducing the expression of annexin A2 by siRNA, the ability of the 2A protein to inhibit apoptosis was weakened and the proliferation of EMCV was slowed down. These results suggest that annexin A2 is closely related to the inhibition of apoptosis by 2A. Furthermore, both RT-PCR and western blot results showed that the 2A protein requires annexin A2 interaction to inhibit apoptosis via JNK/c-Jun pathway. Taken together, our data indicate that the 2A protein inhibits apoptosis by interacting with annexin A2 via the JNK/c-Jun pathway. These findings provide insight into the molecular pathogenesis underlying EMCV infection.


Assuntos
Anexina A2/metabolismo , Apoptose , Vírus da Encefalomiocardite/fisiologia , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Proteínas Proto-Oncogênicas c-jun/metabolismo , Proteínas Virais/metabolismo , Animais , Anexina A2/genética , Apoptose/genética , Linhagem Celular , Cricetinae , Proteínas Quinases JNK Ativadas por Mitógeno/genética , Sistema de Sinalização das MAP Quinases , Ligação Proteica , Proteínas Proto-Oncogênicas c-jun/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Suínos , Proteínas Virais/genética , Replicação Viral
2.
Res Vet Sci ; 85(2): 368-71, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18155114

RESUMO

This study was carried out to investigate the biological characteristics of the foot-and-mouth disease (FMD) virus strain Asia-1 China/2005, which is responsible for the 2005 epidemic in China. The result showed that this strain is not host restricted, and could not only cause FMD in cattle and sheep but also in pigs by either inoculation or direct contact.


Assuntos
Surtos de Doenças/veterinária , Vírus da Febre Aftosa/genética , Febre Aftosa/epidemiologia , Animais , Anticorpos Antivirais/sangue , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , China/epidemiologia , Febre Aftosa/imunologia , Febre Aftosa/virologia , Vírus da Febre Aftosa/classificação , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/virologia , Especificidade da Espécie , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...