Non-bracket oblique traction-hoisting construction strategy for cable-truss structures.

This study describes the non-bracket oblique traction-hoisting construction strategy for cable-truss structures, which is to assemble the upper and lower radial cables, hoop cables, sling cables, and compression rods without stress at a low altitude, then hoist the cable-strut system to a high altitude by oblique traction of the upper radial cables through the jack fixed on the upper radial anchorage nodes, and finally actively tension the lower radial cables to achieve the designed shape and prestress level of the entire structure. This strategy assembles at a low altitude, requires simple operations, results in high tensioning efficiency, and does not require brackets, which could guarantee both quality and quantity in terms of completing the construction of cable-truss structures. The semilune-shaped canopy of Yueqing Stadium is constructed using this strategy. The construction simulation and disturbance stability analyses of the structure in the traction-hoisting state and prestress tensioning state are conducted using a nonlinear dynamic finite element method. In the traction-hoisting stage, the deformation changes sharply, and the hoop cables and upper radial cables make up the primary bearing substructure, while the lower radial cables are in a suspended hanging state. In the forming process, the forces of the radial and hoop cables increase gradually, and the structure finally reaches the designed state. For cable-trusses with crossed upper and lower radial cables, the additional stabilizing tooling ropes should be tied at the top of the middle rods to ensure geometric stability because they are susceptible to excessive out-of-plane displacement or even overturning, which is the least desirable at the beginning of traction hoisting.

Hetero Nucleus Growth Stabilizing Zinc Anode for High-Biosecurity Zinc-Ion Batteries.

Biocompatible devices are widely employed in modernized lives and medical fields in the forms of wearable and implantable devices, raising higher requirements on the battery biocompatibility, high safety, low cost, and excellent electrochemical performance, which become the evaluation criteria toward developing feasible biocompatible batteries. Herein, through conducting the battery implantation tests and leakage scene simulations on New Zealand rabbits, zinc sulfate electrolyte is proved to exhibit higher biosecurity and turns out to be one of the ideal zinc salts for biocompatible zinc-ion batteries (ZIBs). Furthermore, in order to mitigate the notorious dendrite growth and hydrogen evolution in mildly acidic electrolyte as well as improve their operating stability, Sn hetero nucleus is introduced to stabilize the zinc anode, which not only facilitates the planar zinc deposition, but also contributes to higher hydrogen evolution overpotential. Finally, a long lifetime of 1500 h for the symmetrical cell, the specific capacity of 150 mAh g-1 under 0.5 A g-1 for the Zn-MnO2 battery and 212 mAh g-1 under 5 A g-1 for the Zn-NH4V4O10 battery are obtained. This work may provide unique perspectives on biocompatible ZIBs toward the biosecurity of their cell components.

Genome-wide identification of abiotic stress-regulated and novel microRNAs in mulberry leaf.

As the most important food plant for sericultural industry, mulberry trees have to suffer from a wide range of abiotic and biotic stresses, such as drought and high salinity. MicroRNAs (miRNAs) have been proved to play important roles in abiotic stresses regulation in many plants. However, there are seldom reports on the miRNAs expression profiles upon abiotic challenges in mulberry. In this study, three small RNA libraries from mulberry leaf tissue with or without drought or salt treatment were constructed and deep sequenced. Total of 48 conserved miRNAs (including miRNA*) and 162 novel miRNAs were identified (processing precision value>0.1). A total of 270 and 1963 target genes were predicted for conserved miRNAs and novel miRNAs, respectively. 13 differentially expressed miRNAs were detected under drought or salt stresses by deep sequencing and qRT-PCR. 5' RLM-RACE validated Morus 013341 to be the target gene of miR-395a. Our results provided initial clue to further study molecular mechanism on abiotic stresses regulation in mulberry.

Involvement of microRNAs in infection of silkworm with bombyx mori cytoplasmic polyhedrosis virus (BmCPV).

Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the most important pathogens of silkworm. MicroRNAs (miRNAs) have been demonstrated to play key roles in regulating host-pathogen interaction. However, there are limited reports on the miRNAs expression profiles during insect pathogen challenges. In this study, four small RNA libraries from BmCPV-infected midgut of silkworm at 72 h post-inoculation and 96 h post-inoculation and their corresponding control midguts were constructed and deep sequenced. A total of 316 known miRNAs (including miRNA*) and 90 novel miRNAs were identified. Fifty-eight miRNAs displayed significant differential expression between the infected and normal midgut (P value < = 0.01 and fold change > = 2.0 or < = 0.5), among which ten differentially expressed miRNA were validated by qRT-PCR method. Further bioinformatics analysis of predicted target genes of differentially expressed miRNAs showed that the miRNA targets were involved in stimulus and immune system process in silkworm.