RESUMO
Elastin-like polypeptides (ELPs) are stimulus-responsive protein-based biopolymers that exhibit phase transition behavior. By joining them to synthetic phytochelatin (EC), EC-ELP fusion proteins with temperature sensitivity and metal-binding functionality were generated to remove heavy metal ions biologically. Three different EC domains (EC10, EC20, EC30) were incorporated into the ELP, and the EC-ELP fusion proteins were expressed in E. coli. Their thermal properties and metal binding abilities were then investigated according to the EC length. In addition, the feasibility of reusing EC-ELPs and the cadmium ion binding affinity of reused EC-ELPs were explored.
Assuntos
Cádmio/metabolismo , Elastina/metabolismo , Fitoquelatinas/metabolismo , Cádmio/isolamento & purificação , Clonagem Molecular , Elastina/química , Elastina/genética , Escherichia coli/genética , Expressão Gênica , Metais Pesados/isolamento & purificação , Metais Pesados/metabolismo , Peptídeos/química , Peptídeos/genética , Peptídeos/metabolismo , Transição de Fase , Fitoquelatinas/química , Fitoquelatinas/genética , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Temperatura , Poluentes Químicos da Água/isolamento & purificação , Poluentes Químicos da Água/metabolismoRESUMO
The purpose of this study was to determine the effectiveness of antihistamine therapy for withdrawal movements caused by rocuronium injection. One hundred seventy one ASA I-II adults undergoing elective surgery were randomly assigned to one of two groups. Patients in the control group (Group C) were premedicated with 2 mL normal saline, and those in the antihistamine group (Group A) were pre-medicated with 2 mL (45.5 mg) pheniramine maleate. After the administration of thiopental sodium 5 mg/kg, rocuronium 0.6 mg/kg was injected. Withdrawal movements were assessed using a four-grade scale. The administration of antihistamine reveals lower grade of withdrawal movement after rocuronium injection.
