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Degenerative tendon injuries are common clinical problems associated with overuse or aging, and understanding the mechanisms of tendon injury and regeneration can contribute to the study of tendon healing and repair. As a transcription factor, Mohawk (Mkx) is responsible for tendons development, yet, the roles of which in tendon damage remain mostly elusive. In this study, using Mkx overexpressed mice on long treadmill as an in vivo model and MkxOE Achilles tenocytes stimulated by equiaxial stretch as an in vitro model, we anaylsed the effects of Mkx overexpression on the tendon. Mkx and tendon tension strength were decreased after the expose to excessive mechanical forces, and Mkx overexpression protected the tendon from damage. Moreover, we revealed that the Wnt/ß-catenin activation, inflammation, and Runx2 expression were increased at the injured Achilles tendon, upregulated Mkx significantly reversed the increased Wnt/ß-catenin pathway, Tnf-α, Il-1ß, and Il-6 levels, and reduced tendon cell damage. However, Wnt3a, IWR and BIO had not significantly affected the Mkx expression in achilles tenocytes. In conclusion, Mkx is involved in tendon healing and protects the tendon from damage through suppressing Wnt/ß-catenin pathway, suggesting Mkx/Wnt/ß-catenin pathway may be potential therapeutic targets for tendon damage.
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OBJECTIVES: Shifting macrophages towards an anti-inflammatory state is key in treating osteoarthritis (OA) by reducing inflammation and tissue damage. However, the underlying mechanisms guiding this shift remain largely undefined. STUB1, an E3 ubiquitin ligase, known for its regulatory role in macrophage polarization. This study aims to explore the function and underlying action mechanisms of STUB1 in OA. METHODS: An in vivo OA model was established in rats. Hematoxylin-Eosin and safranin O-fast green staining were performed to reveal the hispathological injuries in knee-joint tissues. Immunohistochemistry and flow cytometry were performed to detect the distribution of M1 and M2 macrophages. The inflammatory response (TNF-α and IL-6 levels) was evaluated by ELISA. In vitro, the interaction between STUB1 and NFR2 was determined by CO-IP and pull-down assays. After treated with LPS (an in vitro model of OA), the viability and apoptosis of chondrocytes were measured by CCK-8 and flow cytometry, respectively. RESULTS: Silencing STUB1 alleviated OA in rats, as indicated by reduced subchondral bone thickness, knee synovitis score, histopathological damages, and inflammatory response. STUB1 silencing also decreased M1 macrophages and increased M2 macrophages in both in vivo and in vitro settings. NRF2 was identified as a target of STUB1, with STUB1 mediating its ubiquitination. Silencing NRF2 reversed the effects of STUB1 silencing on inducing M2 macrophage polarization. Furthermore, silencing STUB1 upregulated NRF2 expression in LPS-treated chondrocytes, promoting cell viability and inhibiting apoptosis. CONCLUSION: Silencing STUB1 induces M2 macrophage polarization by inhibiting NRF2 ubiquitination, thereby contributing to the mitigation of OA.
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Fator 2 Relacionado a NF-E2 , Osteoartrite , Animais , Ratos , Inflamação/patologia , Lipopolissacarídeos/farmacologia , Macrófagos/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Osteoartrite/patologiaRESUMO
In clinical practice, anterior cruciate ligament (ACL) rupture is always repaired by the single-beam reconstruction method. Before the surgery, the surgeon made the diagnosis based on medical images, such as CT (computerized tomography) and MR (magnetic resonance) images. However, little is known about how biomechanics governs the biological nature for femoral tunnel position. In the present study, three volunteers' motion trails were captured by six cameras when they were doing squat movement. The medical image can reconstruct the structure of the ligaments and bones and a left knee model was reconstructed by MIMICS by MRI data of DICOM format. Finally, the effects of different femoral tunnel positions on ACL biomechanics were characterized by the inverse dynamic analysis method. The results showed that there were significant differences in the direct mechanical effects of the anterior cruciate ligament at different locations of the femoral tunnel (p < 0.05), the peak stress of ACL in the low tension area was 1097.24 ± 25.55 N, and the peak stress of ACL in the distal femur was 356.81 ± 15.39 N, both of which were much higher than that in the direct fiber area (118.78 ± 20.68 N).
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Ginsenoside Rh2, which is extracted from ginseng, exerts antitumor activity. Recent studies suggest that Rh2 may suppress the growth of colon cancer (CC) in vitro. However, the underlying mechanism remains unclear. In this study, we identified the relative levels of miR-150-3p in CC tissues and cells by a comprehensive strategy of data mining, computational biology, and real-time reverse transcription PCR (qRT-PCR) experiments. The regulatory effects of miR-150-3p/SRCIN1 on the proliferative and invasive abilities of CC cells are evaluated by CCK-8, EdU, wound healing, and transwell assays. Cell cycle- and apoptosis-related protein levels are assessed by western blot analysis. An in vivo tumor formation assay was conducted to explore the effects of miR-150-3p on tumor growth. Furthermore, bioinformatics and dual luciferase reporter assays are applied to determine the functional binding of miRNA to mRNA of the target gene. Finally, the relationship between Rh2 and miR-150-3p was further verified in SW620 and HCT-116 cells. miR-150-3p is downregulated in CC tissues and cell lines. Functional assays indicate that the upregulation of miR-150-3p inhibits tumor growth both in vivo and in vitro. In addition, SRCIN1 is upregulated in CC and predicts a poor prognosis, and it is the direct target for miR-150-3p. Moreover, the miR-150-3p mimic decreases Topflash/Fopflash-dependent luciferase activity, resulting in the inhibition of Wnt pathway activity. Rh2 can suppress the growth of CC by increasing miR-150-3p expression. Rh2 alleviates the accelerating effect on Wnt pathway activity, cell proliferation/migration, and colony formation caused by miR-150-3p inhibition. Rh2 inhibits the miR-150-3p/SRCIN1/Wnt axis to suppress colon cancer growth.
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Neoplasias do Colo , Ginsenosídeos , MicroRNAs , Humanos , Linhagem Celular Tumoral , MicroRNAs/genética , MicroRNAs/metabolismo , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/genética , Ginsenosídeos/farmacologia , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Movimento CelularRESUMO
OBJECTIVES: Unilateral percutaneous kyphoplasty (UPKP) has been effective in reducing the operative time, cement volume, and cement leakage (CL) rate compared with bilateral kyphoplasty. However, no device can help to determine the trajectory during operation, especially the inner inclination angle. To assess the safety and efficacy of a novel guide device (GD) for UPKP in the treatment of thoracolumbar osteoporotic vertebral fractures (TLOVFs). METHODS: From January 2019 to May 2021, 31 patients diagnosed with single TLOVF who underwent UPKP were retrospectively reviewed. The patients were divided into two groups: traditional UPKP (UPKP group, 15 patients) and UPKP assisted with GD (UPKP-GD group, 16 patients). Pre-procedure demographic, clinical and radiologic characteristics, operative procedure details, and clinical and radiologic outcomes at 1 day and 12 months post-procedure were collected. Statistical analyses were carried out using SPSS 24.0. The baseline characteristics of the two groups were compared by the independent sample t test or the χ2 test. The anterior height or local kyphotic angle (LKA) of the fractured vertebrae, visual analog scale (VAS) score, and Oswestry Disability Index (ODI) within groups were compared using the paired t test. RESULTS: A total of 31 patients (five men and 26 women; age range: 58-90 years) completed the full 12-month postoperative follow-up schedule. No significant differences were observed between treatment groups with respect to sex, age, body mass index, preoperative bone mineral density, or surgical level. Compared with the UPKP group, the operation time in the UPKP-GD group was significantly shorter (40.8 ± 5.5 min vs. 48.5 ± 8.5 min, p = 0.005), and the number of intraoperative fluoroscopy times in the UPKP-GD group was significantly decreased (20.6 ± 4.5 vs. 25.2 ± 2.4, p = 0.001). Five (31.3%) patients in the UPKP-GD group and four (26.7%) patients in the UPKP group had bone CL. The VAS and ODI scores, anterior height and LKA of the fractured vertebrae were significantly improved after surgery in each group. No significant differences in postoperative VAS and ODI scores, anterior height or LKA of the fractured vertebrae, volume of injected cement or CL were observed between the two groups. CONCLUSION: Unilateral puncture using a novel GD is a safe and effective technique for patients with TLFs and UPKP assisted with a novel GD is associated with fewer intraoperative fluoroscopy times and shorter operation time.
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Fraturas por Compressão , Cifoplastia , Cifose , Fraturas por Osteoporose , Fraturas da Coluna Vertebral , Masculino , Humanos , Feminino , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Cifoplastia/métodos , Fraturas da Coluna Vertebral/diagnóstico por imagem , Fraturas da Coluna Vertebral/cirurgia , Estudos Retrospectivos , Fraturas por Compressão/cirurgia , Resultado do Tratamento , Punção Espinal , Coluna Vertebral , Cimentos Ósseos/uso terapêutico , Cifose/cirurgia , Fraturas por Osteoporose/cirurgia , Fraturas por Osteoporose/tratamento farmacológicoRESUMO
We investigated the function of lncRNA zinc finger antisense 1 (ZFAS1) in intervertebral disc degeneration (IDD) progression in vitro and in vivo. Nucleus pulposus (NP) tissues were obtained from 20 patients with IDD. IL-1ß was used to stimulate primary NP cells to establish the IDD models in vitro. Gene expression was determined by RT-qPCR. 5-Ethynyl-2'-deoxyuridine and flow cytometry were performed to determine cell proliferation and apoptosis, and western blotting was conducted to measure the apoptosis- and extracellular matrix (ECM)-related protein expression. Luciferase reporter assay was used to examine the interactions between the genes. We also investigated the effect of ZFAS1 in a mouse model of IDD induced by needle punctures. Our results showed that ZFAS1 expression was elevated in degenerative NP tissues and IL-1ß-treated NP cells. ZFAS1 knockdown inhibited NP cell apoptosis and ECM degradation induced by IL-1ß. Mechanically, ZFAS1 sponged miR-4711-5p and adaptor-associated kinase 1 (AAK1) was targeted by miR-4711-5p. Furthermore, AAK1 overexpression partially eliminated the impact of ZFAS1 depletion on NP cell proliferation, apoptosis, and ECM degradation. More importantly, the results of the in vivo studies confirmed the effect of silencing ZFAS1 on alleviating the symptoms of IDD mice. Overall, silencing ZFAS1 inhibits IDD progression by reducing NP cell apoptosis and ECM degradation through the miR-4711-5p/AAK1 axis.
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BACKGROUND: To investigate the clinical application of modified Crain classification in anterior cruciate ligament (ACL) reconstruction (ACLR) with remnant preservation. METHODS: The subjects were 70 patients with ACL injury who underwent ACLR from May 2016 to June 2018, and their general data were recorded. They were randomly divided into modified remnant-preserved ACLR group (group M, n = 35) and non remnant-preserved ACLR group (group N, n = 35). ACLR program with remnant preservation was designed based on modified Crain classification in group M, while ACL remnants were completely cleaned during ACLR in group N. Subsequently, the two groups were compared in terms of operation time, complications, as well as Lysholm score, international knee documentation committee (IKDC) score and positive rate of Lachman test of knee joint before operation and at 3, 6 and 12 months after operation. RESULTS: Both the groups showed good postoperative efficacy, and none had complications like limited knee extension or cyclops lesion. The comparison results found that group M (72.49 ± 7.64 min) required longer operation time than group N (66.06 ± 6.37 min) (P < 0.05). Lysholm score and IKDC score at 3, 6 and 12 months after operation in the two groups were significantly higher than those before operation (P < 0.05); group M had higher Lysholm score and IKDC score at 3 months and 6 months after operation compared with group N (P < 0.05). Additionally, the positive rate of Lachman test at 3, 6 and 12 months after operation in both groups was significantly lower than that before operation (P < 0.05), but there was no significant difference between group M and group N. CONCLUSION: With the modified Crain classification, many remnant-preserved reconstruction techniques can be rationally used to completely preserve the remnant ligament tissue during operation and improve knee joint function and joint stability with few complications.
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Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior , Humanos , Ligamento Cruzado Anterior/cirurgia , Lesões do Ligamento Cruzado Anterior/cirurgia , Reconstrução do Ligamento Cruzado Anterior/métodos , Articulação do Joelho/cirurgia , Resultado do TratamentoRESUMO
The intestinal microflora is a bacterial group that lives in the human digestive tract and has a long-term interdependence with the host. Due to the close anatomical and functional relationship between the liver and the intestine, the intestinal flora affects liver metabolism via the intestinal-hepatic circulation, thereby playing an extremely important role in the pathological process of liver inflammation, chronic fibrosis, and liver cancer. In recent years, the rapid development of technologies in high-throughput sequencing and genomics has opened up possibilities for a broader and deeper understanding of the crosstalk between the intestinal flora and the occurrence and development of liver cancer. This review aims to summarize the mechanisms by which the gut microbiota changes the body's metabolism, through the gut-liver axis, thereby affecting the occurrence and development of primary liver cancer. In addition, the potential regulation of intestinal microflora in the treatment of liver cancer is discussed.
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Carcinoma Hepatocelular , Microbioma Gastrointestinal , Neoplasias Hepáticas , Microbioma Gastrointestinal/fisiologia , Humanos , Cirrose HepáticaRESUMO
OBJECTIVE: This study aimed to explore the epidemiological and microbiological characteristics of fracture-related infection (FRI), analyze the drug resistance characteristics of major pathogens, and provide timely and relatively complete clinical and microbiological data for antimicrobial treatment of FRI. METHODS: The clinical and microbiological data of patients with FRI from January 1, 2011, to December 31, 2020, were collected from three tertiary hospitals in Northeast China. The automatic microbial analysis system was used for strain identification and drug susceptibility testing, and the drug susceptibility results were determined in accordance with the latest Clinical and Laboratory Standards Institute (CLSI) criteria (as applicable each year). RESULTS: A total of 744 patients with FRI were enrolled. The incidence of FRI was about 1.5%, and 81.7% were male patients, with an average age of 48.98 ± 16.01 years. Open fractures accounted for 64.8%. Motor crush (32.8%) and falling (29.8%) were the main causes of injuries. The common sites of infection were the tibia and fibula (47.6%), femur (11.8%), foot (11.8%), and hand (11.6%). A total of 566 pathogenic bacteria were cultured in 378 patients with positive bacterial cultures, of which 53.0% were Gram-positive bacteria and 47.0% were Gram-negative bacteria. The most common pathogen at all sites of infection is Staphylococcus aureus. Staphylococcus aureus had a high resistance rate to penicillin (PEN), erythromycin (ERY), and clindamycin (CLI), exceeding 50%. Methicillin-resistant Staphylococcus aureus (MRSA) was more than 80% resistant to CLI and ERY. CONCLUSIONS: The incidence of FRI in Northeast China was at a low level among major medical centers nationwide. Staphylococcus aureus was still the main pathogen causing bone infections, and the proportion of MRSA was lower than reported abroad, but we have observed an increase in the proportion of infections. Enterobacteriaceae have a higher resistance rate to third-generation cephalosporins and quinolones. For Enterobacteriaceae, other sensitive treatment drugs should be selected clinically.
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Staphylococcus aureus Resistente à Meticilina , Mycobacterium tuberculosis , Preparações Farmacêuticas , Infecções Estafilocócicas , Staphylococcus aureus/química , Adulto , Idoso , Farmacorresistência Bacteriana , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-IdadeRESUMO
Given the rapid development of genome mining in this decade, the substrate channel of paclitaxel might be identified in the near future. A robust microbial cell factory with gene dbat, encoding a key rate-limiting enzyme 10-deacetylbaccatin III-10-O-transferase (DBAT) in paclitaxel biosynthesis to synthesize the precursor baccatin III, will lay out a promising foundation for paclitaxel de novo synthesis. Here, we integrated gene dbat into the wild-type Escherichia coli BW25113 to construct strain BWD01. Yet, it was relatively unstable in baccatin III synthesis. Mutant gene dbat S189V with improved thermostability was screened out from a semi-rational mutation library of DBAT. When it was over-expressed in an engineered strain N05 with improved acetyl-CoA generation, combined with carbon source optimization of fermentation engineering, the production level of baccatin III was significantly increased. Using this combination, integrated strain N05S01 with mutant dbat S189V achieved a 10.50-fold increase in baccatin III production compared with original strain BWD01. Our findings suggest that the combination of protein engineering and metabolic engineering will become a promising strategy for paclitaxel production.
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A novel CFCl2CF2Cl(R113)/H2 gas treatment process was proposed and successfully applied for the redispersion of a sintered alloy PdCu/AC catalyst. The redispersion process was initiated by the formation of an oxidative PdCuClx intermediate, which can easily migrate to a nearby catalyst surface and then be reduced to smaller PdCu NPs.
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Ni2P@C facilely prepared by microwave assisted combustion with phytic acid and nickel nitrate followed by reduction at 650 °C exhibits even higher activity for the hydrodechlorination of HCFC-22 than Pd/C. This method is also applicable for the synthesis of other metal phosphides, providing a versatile method for the fabrication of carbon encapsulated metal phosphide particles.
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Cr2O3 nanoparticles were prepared by solution combustion synthesis (SCS) with chromium nitrate as the precursor and glycine as the fuel. Commercial Cr2O3 and Cr2O3 prepared by a precipitation method were also included for comparison. The morphology, structure, acidity and particle size of fresh and spent Cr2O3 catalysts were investigated by techniques such as XRD, SEM, TEM, BET and NH3-TPD. In addition, catalytic performance was evaluated for the dehydrofluorination of 1,1,1,3,3-pentafluoropropane (CF3CH2CHF2, HFC-245fa) to 1,3,3,3-tetra-fluoropropene (CF3CH=CHF, HFO-1234ze). The catalytic reaction rate of Cr2O3 prepared by SCS method is as high as 6 mmol/h/g, which is about 1.5 times and 2 times higher than that of precipitated Cr2O3 and commercial Cr2O3, respectively. The selectivity to HFO-1234ze for all the catalysts maintains at about 80%. Compared with commercial and precipitated Cr2O3, Cr2O3-SCS prepared by SCS possesses higher specific surface area and acid amount. Furthermore, significant change in the crystal size of Cr2O3 prepared by SCS after reaction was not detected, indicating high resistance to sintering.
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Compostos de Cromo/síntese química , Fluorocarbonos/química , Hidrocarbonetos Fluorados/química , Nanopartículas/química , Catálise , Halogenação , Concentração de Íons de Hidrogênio , Oxirredução , Tamanho da Partícula , Soluções , Propriedades de SuperfícieRESUMO
A defective nanodiamond-graphene material (ND@G) exhibits superior catalytic activity in acetylene hydrochlorination with an acetylene conversion of 50%, selectivity to vinyl chloride of up to 99.5% at 220 °C, and a C2H2 gas hourly space velocity of 300 h-1, which is the first example of a metal-free catalyst with comparable performance to that of the 0.25% Au/C catalyst.
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Nitrogen co-doping with ruthenium mesoporous carbons (Ru-N-MC) was prepared by co-impregnation of sucrose and urea on a RuCl3/SiO2 template followed by a thermal carbonization process. The turnover frequency (TOF) of the Ba/Ru-N-MC catalyst in ammonia synthesis is 0.16 s-1 under reaction conditions of 400 °C, pressure of 10 MPa and space velocity of 10 000 h-1. The superior catalytic performance of the Ba/Ru-N-MC is proposed to originate from the strong metal-support interaction between Ru nanoparticles (NPs) and carbon support. In addition to the activity, the Ba/Ru-N-MC catalyst exhibits a long-term stability for 35 h without significant deactivation.
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In this study, three different approaches, i.e. the sol-gel method, precipitation method and hard-template method, were applied to synthesize MgF2 catalysts with improved stability towards the dehydrofluorination of hydrofluorocarbons (HFCs); the in situ XRD technique was employed to investigate the relationship between the calcination temperature and the crystallite size of precursors to determine optimal calcination temperature for the preparation of the MgF2 catalysts. Moreover, the physicochemical properties of MgF2 catalysts were examined via BET, XRD, EDS and TPD of NH3 and compared. Undoubtedly, the application of different methods had a significant influence on the surface properties and catalytic performances of MgF2 catalysts. The surface areas of the catalysts prepared by the precipitation method, sol-gel method and template method were 120, 215 and 304 m2 g-1, respectively, upon calcination at 200 °C. However, the surface area of the MgF2 catalysts decreased significantly when the calcination temperatures of 300 and 350 °C were applied. The catalytic performance of these catalysts was evaluated via the dehydrofluorination of 1,1-difluoroethane (HFC-152a). The MgF2 catalyst prepared by the precipitation method showed the lowest catalytic activity among all the MgF2 catalysts. When the calcination temperature was above 300 °C, the MgF2 catalysts prepared via the template method demonstrated the highest catalytic conversion rate with catalytic activity following the order: MgF2-T (template method) > MgF2-S (sol-gel method) > MgF2-P (precipitation method). The conversion rate generally agreed with the total amount of acid on the surface of the catalysts, which was measured by the NH3-TPD technique. The MgF2-T catalysts were further examined for the dehydrofluorination of HFC-152a for 600 hours, and a conversion rate greater than 45% was maintained, demonstrating superior long-term stability of these catalysts.
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Subsequent to the publication of the above paper, the authors realize that they did not acknowledge their funders in their paper. Consequently, the following information should have been included in the published article: "Funding: This study was supported by a grant from the National Natural Science Foundation of China (no. 81401586)". The authors sincerely apologize for this mistake, and regret the inconvenience this omission has caused, including that to the funders of this study. [the original article was published in the Molecular Medicine Reports 16: 192-200, 2017; DOI: 10.3892/mmr.2017.6553].
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N-Doped mesoporous carbon extrudate with a major quaternary N species has been successfully prepared through direct carbonization of wheat flour/gluten with silica, which is a cheap and convenient method for scale-up production approach. The obtained carbon extrudate metal-free catalyst enables highly efficient production of vinyl chloride monomer through acetylene hydrochlorination, with a superior catalytic performance and excellent stability (>85% conversion and vinyl chloride selectivity over 99% at 220 °C).
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The present study aimed to investigate the role of Bruton's tyrosine kinase (BTK) in the pathogenesis of lung injury induced by traumahemorrhagic shock (THS), and to examine the pulmonary protective effects of BTK inhibition. Male SpragueDawley rats were divided into four groups (n=12/group): i) A Sham group, which received surgery without induced trauma; ii) a THSinduced injury group; iii) a THSinduced injury group that also received treatment with the BTK inhibitor LFMA13 prior to trauma induction; and iv) a Sham group that was pretreated with LFMA13 prior to surgery but did not receive induced trauma. The expression of phosphorylatedBTK protein in the lungs was measured by immunohistochemistry and western blot analysis. The bronchoalveolar lavage fluid (BALF) protein concentration, total leukocyte and eosinophil numbers, and the expression levels of peripheral blood proinflammatory factors were measured. Morphological alterations in the lungs were detected by hematoxylin and eosin staining. Pulmonary nitric oxide (NO) concentration and inducible NO synthase (iNOS) expression were also assessed. Activities of the nuclear factor (NF)κB and mitogenactivated protein kinase (MAPK) signaling pathways were determined by western blotting or electrophoretic mobility shift assay. BTK was notably activated in lungs of THS rats. BALF protein concentration, total leukocytes and eosinophils, peripheral blood expression levels of tumor necrosis factorα, interleukin (IL)1ß, IL6 and monocyte chemotactic protein 1 were significantly upregulated after THS induction, and each exhibited decreased expression upon LFMA13 treatment. THSinduced interstitial hyperplasia, edema and neutrophilic infiltration in lungs were improved by the inhibition of BTK. In addition, THSinduced NO release, iNOS overexpression, and NFκB and MAPK signaling were suppressed by BTK inhibition. Results from the present study demonstrate that BTK may serve a pivotal role in the pathogenesis of THSrelated lung injury, and the inhibition of BTK may significantly alleviate THSinduced lung damage. These results provide a potential therapeutic application for the treatment of THSinduced lung injury.
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Lesão Pulmonar Aguda/etiologia , Lesão Pulmonar Aguda/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Proteínas Tirosina Quinases/antagonistas & inibidores , Choque Hemorrágico/complicações , Ferimentos e Lesões/complicações , Lesão Pulmonar Aguda/tratamento farmacológico , Lesão Pulmonar Aguda/patologia , Tirosina Quinase da Agamaglobulinemia , Animais , Líquido da Lavagem Broncoalveolar , Modelos Animais de Doenças , Imuno-Histoquímica , Mediadores da Inflamação/metabolismo , Masculino , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Substâncias Protetoras/farmacologia , Proteínas Tirosina Quinases/metabolismo , Ratos , Transdução de Sinais/efeitos dos fármacosRESUMO
Effects of chitosan coating with cinnamon oil on the physiological attributes and preservation quality of China jujube fruits during storage at 4°C for 60 days were investigated. Results indicated that weight loss and decay of jujube fruits were significantly reduced by chitosan-oil coating during the period of 60-day storage, which also exhibited a quite beneficial effect on maintaining the sensory quality for jujube fruits. Meanwhile, the contents of vitamin C and titratable acid decreased to 3.08 mg·g(-1) and 0.342% for the fruits treated by chitosan-oil coating (1.0% + 0.10%), respectively. Polyphenol oxidase, superoxide dismutase, and peroxidase activities were 13.40 U·g(-1), 14.53 U·g(-1), and 63.6 U·g(-1) at the end of storage, respectively. The contents of total soluble phenolics and MDA were 34.51 mg·g(-1) and 19.43 µmol·g(-1) for the combined coating treated samples and control fruits, respectively. These results suggested that the chitosan-oil coating might be recognized as one efficiency technology on the preservation quality of jujube fruits during the storage time.