RESUMO
Cellular aging is characterized by the loss of DNA replication capability and is mainly brought about by various changes in chromatin structure. Here, we examined changes in MCM2-7 proteins, which act as a replicative DNA helicase, during aging of human WI38 fibroblasts at the single-cell level. We used nuclear accumulation of p21 as a marker of senescent cells, and examined changes in MCM2-7 by western blot analysis. First, we found that senescent cells are enriched for cells with a DNA content higher than 4N. Second, the levels of MCM2, MCM3, MCM4 and MCM6 proteins decreased in senescent cells. Third, cytoplasmic localization of MCM2 and MCM7 was observed in senescent cells, from an analysis of MCM2-7 except for MCM5. Consistent with this finding, fragmented MCM2 was predominant in these cells. These age-dependent changes in MCM2-7, a protein complex that directly affects cellular DNA replication, may play a critical role in cellular senescence.
Assuntos
Senescência Celular/genética , Replicação do DNA/genética , Componente 2 do Complexo de Manutenção de Minicromossomo/genética , Complexos Multiproteicos/genética , Proteínas de Ciclo Celular/genética , Regulação da Expressão Gênica/genética , Humanos , Componente 2 do Complexo de Manutenção de Minicromossomo/química , Componente 3 do Complexo de Manutenção de Minicromossomo/genética , Componente 4 do Complexo de Manutenção de Minicromossomo/genética , Componente 6 do Complexo de Manutenção de Minicromossomo/genética , Componente 7 do Complexo de Manutenção de Minicromossomo/genética , Complexos Multiproteicos/química , Análise de Célula Única , Quinases Ativadas por p21/genéticaRESUMO
PROBLEM: Although pharmacotherapy is an effective treatment for many psychiatric disorders in children and adolescents, medication adherence rates among children are low. This study clarifies factors affecting children's medication adherence and the role of parental involvement. METHOD: Patients aged 7-17 years with a history of psychotropic medication treatment and their mothers were included in this study. Each mother and child completed self-administered questionnaires. Thirty mother-child pairs who fully completed the questionnaires were included in the analysis. RESULTS: Medication adherence was greater in children and mothers when mothers felt that "children's symptoms improved with treatment and medication." Medication adherence in children and mothers significantly correlated with the child's reported trust in their parent. CONCLUSIONS: The results suggest the need for psychosocial support that considers the influence of mothers on medication adherence in children treated in child psychiatry departments.
