Prevalence of other autoimmune diseases in polyglandular autoimmune syndromes type II and III.

PURPOSE: Polyglandular autoimmune syndromes (PAS) are complex, heterogeneous disorders in which various autoimmune diseases can occur, affecting both endocrine and non-endocrine organs. In this meta-analysis, the prevalence of associated autoimmune disorders was investigated in PAS II and III. METHODS: A comprehensive search in MEDLINE and Embase databases identified 479 studies with the keywords of PAS II and PAS III. 18 records containing a total of 1312 patients fulfilled our inclusion criteria (original studies reporting at least 10 cases and containing the combination of other autoimmune disorders) and were selected for further analysis. A meta-analysis of prevalence was performed using the random-effects model with the calculation of 95% confidence intervals (CI). Results of each meta-analysis were displayed graphically using forest plots. RESULTS: Distinction between PAS II and PAS III was made in 842 cases, of which 177 and 665 were PAS II and III (21.1 vs 78.9%), respectively. The prevalence of Hashimoto's thyroiditis was significantly higher than that of Graves's disease (39% [95% CI 17-65%] vs. 4% [95% CI 0-10%], respectively; p = 0.001). In PAS II, Addison's disease (AD) coexisted with AITDs, T1DM or the combination of these conditions in 65, 18 and 10% of cases, respectively. In addition, one other endocrine and five non-endocrine organ-specific autoimmune disorders were reported. In PAS III, two other autoimmune endocrinopathies, six non-endocrine organ-specific, and four systemic autoimmune disorders were found in combination with AITDs. CONCLUSIONS: AITDs, T1DM and AD are the most common combinations in PAS, thus screening for these conditions seems to be reasonable.

Digital Eye Strain in a Population of Young Subjects.

The main goal of our study was to prove short-term objective influence of near addition and relieving prism on accommodative and vergence facility in group of young healthy persons. Further, we wanted to prove objective and subjective difference between two types of soft contact lenses during working with digital device. We had in total 37 subjects in our study. The first group contained 37 subjects with average age 23 years without important eye pathology. The second group contained 8 subjects with the same average age without important eye pathology. In the first group of subjects, we measured binocular accommodative facility (BAF) and binocular vergence facility (BVF) with and without addition 1 D and with and without relieving prism 2 pD BO at working distance 45 cm. In the second group, we evaluated BAF, BVF and standardized questionnaire (Computer Vision Syndrome Questionnaire - CVS-Q, Sequí M. 2015) during usage of two types of soft contact lenses. The first type of contact lenses (type 1) contained relieving prism for PC working and type 2 was soft contact lens with aspheric design. In the first group of subjects, we measured these values: BAF without addition = 12.78 cpm, BAF with addition = 11.57 cpm, BVF without prism = 12.32 cpm, BVF with prism = 11.59 cpm. In the second group of subjects, we measured with contact lens type 1 these values: BAF = 12.13 cpm, BVF = 13.63 cpm and questionnaire score 9.43 points. With contact lens type 2 we measured BAF = 11.75 cpm, BVF = 11.63 cpm and questionnaire score 12.86 points. We proved statistically important different between two variable only in the first group between BAF with and without addition and BVF with and without relieving prism. In conclusion, we found statistically important decrease in variable BAF and BVF with usage of addition and relieving prism. We found that neither addition nor prism have positive influence on increase of BAF and BVF. Subjects in second group had higher BAF and BVF values with both types of contact lenses in comparison with natural values. We found that with contact lens type 1 (with addition) subjects had higher BAF and BVF values in comparison with contact lens type 2 (aspheric). Subjects with contact lens type 1 had also lower (better) questionnaire score, i.e. 9.43 versus 12.86 point. Key words: Digital eyestrain syndrome, accommodative and vergence facility, addition, relieving prism.

Sensitivity and specificity of spectral OCT in patients with early glaucoma.

This work deals with sensitivity and specificity in spectral OCT in detection of early glaucoma. Our goal was to evaluate data from RNFL analysis and compare them with resulted diagnosis of glaucoma neuropathy based on visual field changes. This retrospective study contains 31 subjects who undergone OCT examination in our department between years 2008 and 2017. Test statistic showed RNFL OCT sensitivity 63.64 % and specificity 90 %. Test ROC (receiver operating characteristic curve) showed AUC (area under curve) 0.734, which was statistically significant (p = 0.0097). We found that spectral OCT SLO with RNFL analysis is useful and effective instrument in analysis of glaucoma.

Colour vision in group of subjects with and without chromagen filter.

Our aim in this study was to prove influence of chromagen filter on color vision quality. Further we wanted to compare quality of color vision in groups of young healthy persons with persons with maculopathy. In our study we had in total 39 subjects. First group contained 13 subjects with average age 23 years without important eye pathology. In the second group we had 13 patients (average age 68 years) with maculopathy. Third group contained subjects with average age 64 years without important eye pathology. While examination we used sorting tests for color vision: Farnsworth-Munsell test and Lanthony test. Results were evaluated according the Vingryse and King-Smith technique. We found that average total error score (TES) in young healthy subjects with color chromagen filter doesnt differ from TES value gained from patients with disease of macula (p = 0.86). Further we found that chromagen filter changes color vision in group of young subjects on statistical significant level (p = 0.01). But in clinical view this is not important color vision defect (TES = 107.46 and CI = 1.42). Next study result showed statistically not important difference between the color vision in young healthy patient (average age 23 years) and older healthy patients with average age 64 years (p = 0.58). Finally we can conclude that green color chromagen filter doesnt have negative influence for dyslectic patients who will use this filter all day. Further we proved that color vision deterioration of patients with macular disease is not important for practical distinguishing of colors but is important clinically for diagnostic purposes. Last but not least we brought result which shows not important difference between young healthy subjects and older subjects (23 versus 64 years).Key words: Color vision, chromagen lens, total error score, maculopathy.

[Quantitative Color Vision Defect Evaluation - Lanthony Test and its Modified Interpretation]. / Kvantitativní vyhodnocení defektu barvocitu - Lanthonyho test a jeho modifikovaná interpretace.

UNLABELLED: Our aim was to develop quick and exact instrument for examination of color vision defects (CVD). We used Lanthony saturated and desaturated test. Data were evaluated according the Vingrys and King-Smith study. We had together 123 eyes of 86 patients. From all subjects we received these average values: AA 44.32 (min -87.13, max 80.64), TES 13.36 (min 8.84, max 30.30), SI 1.97 (min 1.22, max 5.69) and CI 1.66 (min 1.0, max 3.94). At the base of counting algorithm and average values form saturated and desaturated test we revealed 25 (29 %) patients with CVD. Twelve patients (14 %) classified as CVD+ had dichromacy and all had inborn CVD. Eight patients (9 %) from this group had deutranopia and four patient (5 %) protanopia. Anomaly trichromacy we revealed in thirteen patients (15 %). Eight (9 %) of these patients had inborn CVD. Six (7 %) of these patients had protanomalia, one (1 %) had deuteranomalia and one tritanomalia. We established and specified TES, CI and SI critical values, which was used to dividing patients into specific groups. KEY WORDS: Lanthony test, color vision defect, index of selectivity, index of confusion, total error score.

[Disorders of Simple Binocular Vision in Heterophoria and their Spectacle Correction]. / Poruchy jednoduchého binokulárního videní u heteroforií a jejich resení brýlovou korekcí.

This study deals with evaluation of binocular vision in group of young subjects without eye pathology. We examined at whole 68 subjects with average age 26 years, median 24 years. The sample was divided into two main groups. Group A contains subjects with far and near ortophoria (at whole 26 subjects = 38 %). Group B contains subjects with binocular vision disorder (at whole 42 subjects). One subject had strabismus. The most frequent non-strabismus disorder of binocular vision was convergence insufficiency (13 subjects = 19 %), simple esophoria (12 subjects = 17 %) and simple exophoria (8 subjects = 12 %). We decreased average distance heterophoria value (from 1.02 to 0.36 cm/m esophoria) and near heterophoria value (from 0.60 to 0.31 cm/m exophoria) after proper sphere-cylindrical correction. Result of our study shows that adequate and actual sphere-cylindrical correction can reduce disorder of simple binocular vision.Key words: simple binocular vision, heterophoria, spectacle lenses, convergence insufficiency.

[Mammotomy, new technique not only for breast cancer]. / Mamotomie, jedna z nových vysetrovacích metod nejen pro diagnostiku tumoru prsu.

BACKGROUND: Vacuum biopsy device (mammotome) was primary developed for invasive diagnosis of the mammary gland. METHODS AND RESULTS: In Radiological department of Masaryk Memorial Cancer Institute we use the mammotome not only for breast tissue sampling. CONCLUSIONS: The device is used also for extraction of tumor specimens in advanced epithelial, mesenchymal or the other histological type of tumors for further chemoresistance testing.

Generation of dendritic cells using cell culture bags--description of a method and review of literature.

Anticancer immunotherapy using dendritic cell-based vaccines is a strategy aimed at the induction and maintenance of immune responses against cancer cells. Clinical applications of dendritic cells (DCs) require stringent adherence to Good Manufacturing Practice (GMP) methods and rigorous standardization of DC-based vaccine preparation. Recently, closed systems for DC culture have been developed with a goal to minimize the risk of contamination. Here, we compare the yield, immunophenotype, and functional properties of DCs generated in Lifecell X-Fold culture bags and in plastic wells, both from adherence-selected monocytes, and review the current literature on closed systems for DC generation. We found that both the overall yield and the yield of CD83+ cells in cell culture bags was lower than in the standard culture method. No statistically significant differences were observed in the expression of DC immunophenotypic markers. The capability of DCs cultured in bags and in wells to induce the proliferation of allogeneic mononuclear cells were equivalent. The performance of DCs in mixed lymphocyte reaction correlated significantly (p = 0.005) with the CD83 expression but not with the CD80, CD86, HLA-DR, CD1a, and CD1c expression. We conclude that the immunophenotype and stimulatory properties of DCs cultured in closed cell culture bags are similar to those generated by conventional method using cell culture wells.

Separation of steroid compounds by overloaded preparative chromatography with precipitation in the fluid phase.

In order to gain a commercially acceptable yield compared to the adsorption capacity of the column, a rather large amount of sample is separated with preparative chromatography. During the competitive adsorption of the sample-components, the species adsorbing better can force the rest out of the adsorbent phase. As a consequence, the concentrations of the later components may increase in the fluid phase to a level that those species start to precipitate. A mathematical model which takes the precipitation and dissolution into consideration is presented in this paper. Data calculated by this model are compared to that we obtained by using a previous mathematical model as well as the experimental results gained by a laboratory scale separation of steroid compounds on an organic polymer adsorbent. The equilibrium adsorption-desorption and equilibrium precipitation-dissolution is the first approximation of the complicated process mentioned above. In our next publication we are to extend the model with kinetic terms belonging to adsorption-desorption and precipitation-dissolution process respectively.

Preparative-scale amino acid separation by thermal parametric pumping on an ion-exchange resin.

Thermal parametric pumping was experimentally investigated for the concentration and separation of amino acids. Previous theories of parametric pumping were improved by taking into account dissociation equilibria in the liquid phase. Experiments were carried out with a mixture of glutamic acid, aspartic acid, serine and threonine in a highly acidic solution (HCl). A multi-component equilibrium model was mainly used to simulate the experimental results and to investigate the effect of chloride concentration over a wide range. It is shown that it is always possible to concentrate the amino acids and to separate some of them under certain conditions.

Left ventricular filling in endurance-trained subjects.

Whether exercise-induced increases in left ventricular mass can alter left ventricular diastolic function was evaluated by measuring transmitral flow velocities at rest by Doppler echocardiography in 15 amateur endurance-trained runners and 15 age- and sex-matched sedentary control subjects. Ventricular mass index, end-diastolic volume index and stroke volume index were derived from measurements of M-mode echocardiograms recorded under two-dimensional guidance. All three variables were increased in the runners (p less than 0.01). These findings, plus the lower heart rate at rest (p less than 0.001), were consistent with endurance training. Although the runners had an almost twofold greater myocardial mass index, their peak early diastolic filling velocity and time to peak filling velocity did not differ from those of the sedentary subjects. In runners, the peak filling velocity with atrial systole tended to be lower (p = 0.12), the ratio of peak filling velocity with atrial systole to that of early diastole was less (p less than 0.05) and the percent of stroke volume contributed by atrial systole was less (p less than 0.001). These differences in atrial filling may be related to the lower heart rates at rest in runners. In summary, significant increases in left ventricular mass, when associated with endurance training, do not alter the early diastolic filling of the left ventricle.

[Neurogenic muscular lesion presenting a picture of "central core disease"]. / Neurogenni svalová léze s obrazem "central core disease".

A neurogenic lesion has been described in mother and son. In the son there was a prevalence of denervation changes whereas reinervation ones prevailed in the mother. In both, "central cores" were present in aggregations of type-1 fibres. In the authors' opinion the findings represent a neurogenic lesion (also verified clinically and electromyographically), less advanced in the son and more advanced in the mother, ultimately stabilizing to yield a pattern resembling that of the "central core disease".