Daily use of dentifrice with and without xylitol and fluoride: effect on glucose retention in humans in vivo.

The effect of daily use of three different dentifrices on glucose retention after glucose mouth rinsing was tested in this study regarding xylitol and fluoride. Six experimental groups used three different dentifrices produced by two different companies: xylitol- and fluoride-containing dentifrice (XF), non-xylitol- and fluoride-containing dentifrice (F), and non-xylitol- and non-fluoride-containing dentifrice (NonX-NonF). Subjects were divided at random and rinsed their mouths for 15s with 20ml of 0.5M glucose solution. Glucose and lactate retention were determined by collecting samples of saliva from the approximal areas of the maxillary and mandibular anterior teeth and using the enzyme membrane test. Samples were collected 0, 1 and 2 months after the start of regular dentifrice use. There were significant differences in glucose retention in relation to the dentifrice used, month of sampling, site of sampling, and time since start of rinsing. Their contribution ratios were 2.0, 4.4, 11.7 and 7.4%, respectively (P<0.01). There were significant differences observed between the XF and NonX-NonF groups, with the XF group presenting lower glucose retention than the NonX-NonF group. The XF group presented lower glucose retention than the F group. The F group showed lower glucose retention than the NonX-NonF group. There were significant differences in lactate retention in relation to the month and site of sampling, and their contribution ratios were 3.3 and 2.8%, respectively (P<0.01). There were, however, no significant differences in glucose and lactate retention in relation to the dentifrice manufacturer. It was concluded that the XF dentifrice was the most effective, and the F dentifrice was more effective in reducing glucose retention than the NonX-NonF dentifrice.

Use of fluoridated dentifrice and glucose retention at the approximal areas of anterior teeth.

The present study was undertaken to learn to what extent the use of dentifrice with fluoride affected glucose retention after glucose rinsing. Fifty-eight subjects consisting of forty male adults and eighteen female adults whose average age was 21.9 years were divided at random into two groups; one group used fluoridated dentifrice and the other group used nonfluoridated dentifrice. Subjects rinsed their mouths for fifteen seconds with 20 mL of 0.5 M glucose solution. Samples of saliva were taken from the approximal areas between the right and left central incisors of the maxilla and mandible by inserting the tip portion of a paper point three-minutes after the glucose rinsing, sample of saliva were taken from the neighboring approximal areas of the left central and left lateral incisors. Glucose content of the paper points was determined using the enzyme membrane method. Determinations were made before and at the end of the first, third, and fifth month over a five-month period. Fluoride concentrations in the resting saliva were also determined in different months during this period. At three-minutes and six-minutes, glucose retention in the group using fluoridated dentifrice was found to decrease slightly throughout the experiment; while glucose retention in the group on nonfluoridated dentifrice leveled off or increased in the fifth month. Fluoride concentration in the resting saliva increased during the experiment in the group using fluoridated dentifrice; while fluoride concentration in the group using nonfluoridated dentifrice did not change. It was concluded that the regular use of fluoridated dentifrice made glucose retention slightly lower and fluoride level in whole saliva higher.

Oral glucose retention, saliva viscosity and flow rate in 5-year-old children.

There are significant differences of glucose retention in site-specificity and individuals. Sixty-two 5-year-old nursery schoolchildren participated in this study on the relation between the viscosity of saliva and flow rate and glucose retention. Each child was instructed to rinse his/her mouth with a glucose solution (0.5 M, 5 ml) and then to spit out. Three minutes after rinsing, glucose retention was determined. Resting saliva was collected by a natural outflow method, then the flow rate was determined. A rotational viscometer was used to determine the viscosity. Glucose retention and flow rate were correlated at the left maxillary primary molars, and glucose retention and viscosity were correlated at the maxillary central primary incisors. It was concluded that glucose retention after glucose mouth rinsing was site-specific, and that glucose retention and the index of decayed, missing and filled primary teeth (dmft) were slightly correlated with the salivary viscosity and flow rate.

Glucose retention on the surfaces of primary teeth in 3- and 4-yr-old children.

Glucose retention was determined in 38 kindergarten children ages 3-4 yr. The children rinsed their mouths with 10 ml of a 0.5 mol/l glucose solution for 15 s and then spat out. Three minutes after they put the solution in their mouths, a small paper-point was used to collect samples of saliva from the labial and buccal surfaces of the maxillary and mandibular primary teeth. The concentration of glucose in the small amount of saliva collected was measured with an immobilized enzyme system. Glucose retention was highest on the maxillary central primary incisor, second highest on the maxillary first primary molar and third highest on the maxillary lateral primary incisor. An intermediate value was seen on the maxillary and mandibular second primary molars, the mandibular first primary molar and the maxillary primary canine. A lower value was observed on the mandibular primary canine and the lowest on the mandibular incisors. It was concluded that there were site differences in glucose retention on primary teeth of 3- and 4-yr-old children.

Glucose clearance from different surfaces of human central incisors and first molars.

The study examined the glucose clearance (retention) in saliva at different surfaces of these teeth in 23 subjects. The mouth was thoroughly rinsed for 15 s with 20 ml of a 0.5 M glucose solution. The concentrations of glucose absorbed by small pieces of paper, placed on the mesial, distal, labial (buccal), lingual and occlusal surfaces 3 min after rinsing, were measured using an immobilized enzyme system and an electrochemical sensor. On the maxillary and mandibular central incisors, the glucose concentrations on the labial surfaces were significantly higher than on all other surfaces and lowest on the lingual surfaces. In the mandibular molars, glucose concentrations were significantly higher on the buccal surfaces than on the lingual surfaces. Clear site-specific differences in glucose clearance were thus observed at the different tooth surfaces. It is considered that the differences in the glucose retention rate might be indicative of factors important for the site specificity of dental caries.