RESUMO
(S) -3-Chloro-1-(2-thienyl)-1-propanol was synthesized by the asymmetric reduction of 3-chloro-1-(2-thienyl)propanone with liquid-core immobilized Candida pseudotropicalis 104. The optimum time was 28 h for the re-cultivation of immobilized cells. The optimum film solvent for the liquid-core capsule was 0.3 % chitosan (M (w) 1.0 × 10(5)). Conversion decreased with the increase of the liquid-core capsule diameter and with the addition of more substrates at the same reduction time. The immobilized cells show good reduction ability in a potassium phosphate buffer (pH 6.6~7.2). The material outside the spread speed of immobilized cells was not restricted when the shaking speed was higher than 160 r/min. Liquid-core immobilized cells can be reused 11 times. Compared with the batch reduction, the continuous reduction of 3-chloro-1-(2-thienyl)propanone in the membrane reactor with liquid-core immobilized cells as catalyst can relieve the inhibition from a high-concentration substrate. Conversion and enantiometric excess of (S)-3-chloro-1-(2-thienyl)-1-propanol reached 100 % and >99 % in a continuous reduction of 12 g/L 3-chloro-1-(2-thienyl)propanone for 10 days.
Assuntos
Candida/citologia , Candida/metabolismo , Propanóis/química , Propanóis/metabolismo , Tiofenos/química , Tiofenos/metabolismo , Reatores Biológicos/microbiologia , Soluções Tampão , Cápsulas , Proliferação de Células , Células Imobilizadas/metabolismo , Quitosana/química , Cloridrato de Duloxetina , Concentração de Íons de Hidrogênio , Membranas Artificiais , Peso Molecular , Oxirredução , Fosfatos/química , Compostos de Potássio/químicaRESUMO
Objective To explore the changes of finger artery hemodynamics of patients with pulmonary heart disease, at the same time to search for the relationship with PaO_2.Methods We used American ACUSON128/xp10 and ATL5000 color Doppler ultrasound diagnostic instrument to detect double middle - finger artery hymodynamics items; VSMANX, VDMIN,TAMX , PI and RI. We used Danish ABL5 artery blood gas analysis to monitor PaO_2 and SaO_2.Results PaO_2 ,SaO_2, VSMAX,VDMIN ,TAMX,and PI in control group,pulmonary heart disease remission stage, active attack stage had significant difference (P
