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1.
Medicine (Baltimore) ; 95(35): e4777, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27583932

RESUMO

Since the evidence regarding the association between maternal hepatitis C virus (HCV) infection and impaired intrauterine fetal growth had not been conclusive, the aim of the present study was to evaluate the risk of maternal HCV infection in association with intrauterine fetal growth restriction (IUGR) and/or low birth weight infants (LBW). We performed an extensive literature search of PubMed, MEDLINE, and EMBASE through December 1, 2015. The odds ratios (ORs) of HCV infection and IUGR/LBW were calculated and reported with 95% confidence intervals (95% CIs). Statistical analysis was performed using RevMen 5.3 and Stata 10.0. Seven studies involving 4,185,414 participants and 5094 HCV infection cases were included. Significant associations between HCV infection and IUGR (OR = 1.53, 95% CI: 1.40-1.68, fixed effect model) as well as LBW were observed (OR = 1.97, 95% CI: 1.43-2.71, random effect model). The results still indicated consistencies after adjusting for multiple risk factors which could affect fetal growth, including maternal age, parity, maternal smoking, alcohol abuse, drugs abuse, coinfected with HBV/HIV and preeclampsia. Our findings suggested that maternal HCV infection was significantly associated with an increased risk of impaired intrauterine fetal growth. In clinical practice, a closer monitoring of intrauterine fetal growth by a series of ultrasound might be necessary for HCV-infected pregnant population.


Assuntos
Retardo do Crescimento Fetal/virologia , Hepatite C/complicações , Complicações Infecciosas na Gravidez , Alcoolismo/complicações , Coinfecção , Feminino , Humanos , Recém-Nascido de Baixo Peso , Recém-Nascido , Idade Materna , Paridade , Gravidez , Fatores de Risco , Fumar/efeitos adversos , Transtornos Relacionados ao Uso de Substâncias/complicações
2.
Cell Physiol Biochem ; 38(5): 1860-8, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27160398

RESUMO

BACKGROUND/AIMS: Preeclampsia is a complex multi-system obstetric syndrome and remains one of the leading causes contributing to maternal and perinatal mortality and morbidity. Previous epidemiological studies regarding the association between chronic hepatitis B virus (CHB) infection and the risk of preeclampsia have reported inconsistent results. Therefore, we conducted a meta-analysis to investigate the association between CHB infection and preeclampsia. METHODS: The electronic database was searched until January 1st, 2016. Relevant studies reporting the association between CHB infection and the risk of preeclampsia were included and for further evaluation. Statistical analysis was performed using Stata 10.0 (Stata Corp). RESULTS: Three observational cohort studies and eight case-control studies, including 11566 preeclampsia patients, were identified. A significant negative association between CHB infection and preeclampsia was observed (odds ratio = 0.77, 95% confidence interval, 0.65- 0.90, P=0.002, fixed-effect model). CONCLUSIONS: Findings from our meta-analysis indicate that CHB infection may decrease the risk of preeclampsia in Asian population. Future prospective cohorts in different countries with larger sample sizes are warranted to ascertain the causality and pathophysiological studies are required to explore the possible biological mechanisms involved.


Assuntos
Hepatite B Crônica/complicações , Pré-Eclâmpsia/etiologia , Estudos de Casos e Controles , Bases de Dados Factuais , Feminino , Humanos , Estudos Observacionais como Assunto , Razão de Chances , Gravidez , Risco
3.
Cell Physiol Biochem ; 35(4): 1654-62, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25824463

RESUMO

BACKGROUNDS/AIMS: Preeclampsia was characterized by excessive thrombin generation in placentas and previous researches showed that thrombin could enhance soluble Fms-like tyrosine kinase 1 (sFlt-1) expression in first trimester trophoblasts. However, the detailed mechanism for the sFlt-1 over-production induced by thrombin was largely unknown. The purpose of this study was to explore the possible signaling pathway of thrombin-induced sFlt-1 production in extravillous trophoblasts (EVT). METHODS: An EVT cell line (HRT-8/SVneo) was treated with various concentrations of thrombin. The mRNA expression and protein secretion of sFlt-1 in EVT were detected with real-time polymerase chain reaction and ELISA, respectively. The levels of intracellular reactive oxygen species (ROS) production were determined by DCFH-DA. RESULTS: Exposure of EVT to thrombin induced increased intracellular ROS generation and overexpression of sFlt-1 at both mRNA and protein levels in a dose dependent manner. Short interfering RNA (siRNA) directed against PAR-1 or apocynin (an inhibitor of NADPH oxidase) could decrease the intracellular ROS generation and subsequently suppressed the production of sFlt-1 at mRNA and protein levels. CONCLUSIONS: Our results suggested that thrombin increased sFlt-1 production in EVT via the PAR-1 /NADPH oxidase /ROS signaling pathway. This also highlights the PAR-1 / NADPH oxidase / ROS pathway might be a potential therapeutic target for the prevention of preeclampsia in the future.


Assuntos
NADPH Oxidases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptor PAR-1/metabolismo , Transdução de Sinais/efeitos dos fármacos , Trombina/farmacologia , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Acetofenonas/farmacologia , Linhagem Celular , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , NADPH Oxidases/antagonistas & inibidores , Pré-Eclâmpsia/metabolismo , Pré-Eclâmpsia/patologia , Gravidez , Interferência de RNA , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Receptor PAR-1/antagonistas & inibidores , Receptor PAR-1/genética , Trofoblastos/citologia , Trofoblastos/efeitos dos fármacos , Trofoblastos/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/genética
4.
Gynecol Endocrinol ; 31(5): 337-44, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25703255

RESUMO

Retinol-binding protein 4 (RBP4) is a novel adipocyte-derived cytokine playing an important role in the regulation of energy metabolism and insulin sensitivity. Although the association between RBP4 and metabolic dysfunction is well established, studies on the relationship between circulating RBP4 levels and the risk of gestational diabetes mellitus (GDM) have yielded inconclusive results. We performed a meta-analysis to investigate whether women with GDM had higher circulating RBP4 levels than the normglycemic pregnant women. PubMed, Web of Science and EMBASE were searched up to 1 August 2014. A total of 14 studies comprised of 884 women with GDM and 1251 normglycemic pregnant women were included. The overall results suggested that maternal circulating RBP4 levels were significantly higher in GDM than their normal controls (SMD: 0.49 µg/ml, 95% CI: 0.23-0.75 µg/ml, p < 0.001, random effect model). However, stratified results indicated that this significant difference only existed in the second/third trimester and was limited to Asian populations. Furthermore, subgroup analysis according to matched maternal age and BMI still demonstrated that GDM had higher circulating RBP4 levels than the normal controls. Our findings suggested that Asian women with GDM had increased circulating RBP4 levels in their second/third trimester.


Assuntos
Diabetes Gestacional/sangue , Proteínas Plasmáticas de Ligação ao Retinol/metabolismo , Povo Asiático , Feminino , Humanos , Estudos Observacionais como Assunto , Gravidez , Segundo Trimestre da Gravidez , Terceiro Trimestre da Gravidez , População Branca
5.
J Clin Virol ; 61(1): 3-8, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24973811

RESUMO

Many epidemiological studies have found a positive association between chronic hepatitis B virus (CHB) infection and the risk of preterm labor, but the magnitude of this association varies and independent studies have reported conflicting findings. We performed a meta-analysis to ascertain the relationship between CHB infection and preterm labor. The PubMed and Embase databases were searched up to May 1st, 2014, for relevant observational studies on an association between CHB infection and the risk of preterm labor. Data were extracted and analyzed independently by two authors. The meta-analysis was performed using Stata version 10.0 software. Six observational case-control studies and 4 cohort studies, involving 6781 women with preterm labor, were identified. Based on a random-effects meta-analysis, no association between CHB infection and preterm labor was identified (odds ratio=1.12, 95% confidence interval CI, 0.94-1.33). Our meta-analysis suggested that CHB infection is not associated with an increased risk of preterm labor.


Assuntos
Hepatite B Crônica/complicações , Trabalho de Parto Prematuro/epidemiologia , Feminino , Humanos , Gravidez , Medição de Risco
6.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-325535

RESUMO

<p><b>OBJECTIVE</b>Detecting hepatitis B virus large surface protein (HBLP) with serological method to filtrate the occult HBV infection and study the clinical detection strategy.</p><p><b>METHODS</b>Two thousands HBsAg negative stochastic serum samples were collected from the copy tubes in daily work to detect hepatitis B Virus markers (HBVM) with national EHSA regent kits and put them -20 degrees C frostily. The 2000 samples were detected with HBLP and filtrated the positive samples. The HBsAg markers were doubly counterchecked with other two adding kinds of national ELISA regent kits (total 3 kinds) at the filtrated samples. The last samples were doubly tested again with American MONOLISA HBsAg ULTRA regents. HBV DNA levels were quantitative analyzed with fluorescence quantitative PCR (FQ-PCR) and taking the mean results.</p><p><b>RESULTS</b>Fifteen HBLP positive samples were detected out from the 2000 serum samples. We educed the conform negative results from the three kinds of national regents but conform positive results from the American MONOLISA HBsAg ULTRA regents in repeating HBsAg detection at the 15 samples. The HBV DNA FQ-PCR quantitative results were all less than 500 copies/mi and divided into two cases hetween 400-500 copies/nil, three cases 300-400 copies/ml, five cases 200-300 copies/ml, four cases 100-200 copies/ml and one case was less than l00copies/ml.</p><p><b>CONCLUSION</b>The false HBsAg negative results for serum samples are more generally through national regents than through importations and HBLP results mayhe are positive in these samples. Detecting HBLP marker is propitious to filtrate the occult HBV infection. This study provided a kind of serological reference for actively searching for the detecting strategy in occult HBV infection field.</p>


Assuntos
Feminino , Humanos , Masculino , Ensaio de Imunoadsorção Enzimática , Métodos , Hepatite B , Sangue , Diagnóstico , Virologia , Vírus da Hepatite B , Genética , Proteínas do Envelope Viral , Sangue
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