Experimental study of the effects of Uyghur sand therapy on the ultrastructure of knee articular cartilage in osteoarthritis / 中华物理医学与康复杂志

Objective To study the effects of sand therapy on the ultrastructure of knee articular cartilage in rabbits with osteoarthritis ( OA ) and provide evidence of the effectiveness of sand therapy for OA in Uyghur medicine. Methods Models of knee OA were established by fixing with gypsum the right hind limbs of 28 rabbits. Of these, twenty-six rabbits were divided randomly into a free movement group and a free movement plus sand therapy group, and their left hind limbs were used as normal controls. Another two rabbits were used as specimens. The thirteen rabbits in the free movement group after the gypsum fixings were removed were allowed free movement every day for 20 days; the thirteen rabbits in the free movement plus sand therapy group had free movement plus sand therapy every day. The ultrastructure of each rabbit' s knee articular cartilage was observed using an electron microscope. Results Models of knee OA were established successfully. The changes in the ultrastructure of the knee articular cartilage revealed cell swelling, cell debris in part of the cartilage lacunae, and cartilage cells proliferating. The matrix of cartilage cells was thin, hetero chromatin concentrated on the margin and presented in a crescent form. Cell organellae decreased obviously, which was a sign of apoptosis. After the gypsum fixings were removed, the proliferation of cartilage cells gradually increased and rough endoplasmic reticula increased remarkably in the articular cartilages of the right hind knees of the free movement group. Cell pycnosis, disintegration and necrosis could be found in a few cells. The movement of the affected knees of the group treated with sand therapy plus free movement improved more significantly , and the changes in cartilage ultrastructure revealed more cell active proliferation, more rough endoplasmic reticu-lum, more cell surface processes and more matrix fibers. The morphology of cartilage cells in the control knees remained normal. Conclusion It is confirmed that Uyghur sand therapy combined with movement can improve the ultrastructure of knee articular cartilage in a rabbit model of OA.

Growth promotion of vegetative gametophytes of Undaria pinnatifida by blue light.

Through an acclimation period of 10 days, compared to white light, the maximal net photosynthetic rates were significantly higher for gametophytes of Undaria pinnatifida cultivated under blue light (400-500 nm), and were lower under red light (600-700 nm). Chlorophyll c and the carotenoid content of gametophytes were similar under blue light and red light but were much lower under white light. The growth rate of female gametophytes under blue light was higher than that under other lights, and the growth rate of male gametophytes showed little variation with respect to blue and white light. Male and female gametophytes were mixed together to form sporophytes under white, blue and red light. After approximately 5 days, 50% gametophytes became fertile under blue and white light, but remained vegetative under red light after 10 days.

Morphological and ultrastructural changes of the neuro-endocrine-immune network in the tumor transplantation animal model of abnormal Savda syndrome / 中华中医药杂志

Objective:Abnormal Savda syndrome transplantation tumor model was established on the basis ofabnormal Savda syndrome model in order to study the relevance between the abnormal Savda syndrome tumor and the disorder ofthe neuroendocrine-immune network by analyzing morphological and ultrastructural changes ofthe hypothalamus-pituitary-adrenal axis(HPAA).Methods:120 ICR mice were divided randomly into four groups(control, tumor model, abnormal Savda syndrome model, and abnormal Savda syndrome tumor transplantation model).The structural changes in hypothalamus, pituitary and adrenal glands were analyzed by bright-field and electron microscopy.Results:First, in abnormal Savda syndrome, the rate oftumor transplantation was increased significantly than in the normal state(93.3% and 56.7%, respectively, P

Bile duct injury during open cholecystectomy; an analysis of 29 cases / 中国普通外科杂志

ObjectiveTo find the cause of bile duct injury during open cholecystectomy and evaluate its management. MethodsClinical materials of 29 cases suffering from bile duct injury during conventional cholecystectomy were retrospectively analyzed. Results All patients underwent choledochocholedochostomy or Roux en Y operation. The result was satisfactory in 26 patients, recurrent bile duct stricture developed in 3 patients necessitating a further operation.ConclusionA better expertise is mandatory in the prevention of bile duct injury. The injury should be suspected and identified timely. Different surgical procedures may be suitable in individual patients according to the degree and the site of injury.