RESUMO
World-wide epidemiological studies have shown that cancer of the uterine cervix is the second most common malignant disease in women. Virtually every cervical cancer (99.7%) is HPV-positive, indicating that the presence of HPV is an obligatory element in their development. The present study was conducted by Fast-PCR (within 15 min.) based diagnosis of HPV 16 and HPV 18 infection amongst patients of suspected cervical cancer, confirmed by cytological methods. Twelve women, out of a total of fifty studied cases who had positive cervical pap smears (24%) were found to be positive for HPV 16/HPV 18 infection when PCR based technique was applied. The results indicate, perhaps, a greater specificity of PCR based diagnosis, or presence of other HPV subtypes as etiological factors in the present study group confined to Southern Assam.
Assuntos
Papillomavirus Humano 16/genética , Papillomavirus Humano 18/genética , Programas de Rastreamento , Infecções por Papillomavirus/diagnóstico , Infecções Tumorais por Vírus/diagnóstico , Displasia do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Adulto , Idoso , DNA Viral/genética , Feminino , Seguimentos , Papillomavirus Humano 16/isolamento & purificação , Papillomavirus Humano 18/isolamento & purificação , Humanos , Índia , Pessoa de Meia-Idade , Infecções por Papillomavirus/genética , Infecções por Papillomavirus/virologia , Reação em Cadeia da Polimerase , Prognóstico , Infecções Tumorais por Vírus/genética , Infecções Tumorais por Vírus/virologia , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/virologia , Displasia do Colo do Útero/genética , Displasia do Colo do Útero/virologiaRESUMO
When membranes of Bacillus licheniformis MC14 were extracted exhaustively with 1 M magnesium, approximately 80% of the membrane-associated alkaline phosphatase (orthophosphoric-monoester phosphohydrolase [alkaline optimum], E.C. 3.1.3.1) was solubilized. The remaining activity could be extracted with a cationic detergent, hexadecylpyridinium chloride, without loss of enzymatic activity. The detergent-extractable alkaline phosphatase was immunoprecipitable with antibody to the salt-extractable alkaline phosphatase or the secreted alkaline phosphatase, had an approximate molecular weight of 60,000, and was localized 100% on the outer surface of the cytoplasmic membrane.
Assuntos
Fosfatase Alcalina/análise , Bacillus/enzimologia , Fosfatase Alcalina/isolamento & purificação , Bacillus/ultraestrutura , Membrana Celular/enzimologia , Detergentes , Técnicas Imunoenzimáticas , Peso MolecularRESUMO
Growth conditions affect the quantity and distribution of alkaline phosphatase (orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1) in Bacillus licheniformis MC14. The soluble alkaline phosphatase, which has been found in biochemical localization studies between the cell wall and cell membrane (Glynn, J.A., Schaffel, S.D., McNicholas, J.M. and Hulett, F.M. (1977) J. Bacteriol. 129, 1010-1019), was localized via electron microscope histochemistry in cells cultured under conditions which result in increased quantities of this activity. This soluble alkaline phosphatase was stabilized with 20% glycerol and purified to homogeneity as determined by sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis. The purified enzyme is soluble in dilute buffer. This soluble alkaline phosphatase has been characterized and compared to the membrane-associated alkaline phosphatase from this organism.
