Long-distance transit alters liver and skeletal muscle physiology of beef cattle.

Transportation of cattle is necessary but negatively impacts animal health and production efficiency. To gain a better understanding of the physiological responses to long-distance road transit, 36 crossbred beef steers (324 ±â€¯36 kg) were randomly assigned to treatments (n = 12 steers/treatment): no transit and ad libitum access to feed and water (CON), no transit but deprived of feed and water for 18 h (DEPR), or road transit and no access to feed or water for 18 h (1 790 km; TRANS). Blood, liver, and muscle (longissimus dorsi) samples were collected pre- and post-treatment for analysis of blood metabolites, blood leukocyte profiles, blood markers of oxidative stress, and tissue antioxidant enzyme activity. Additionally, discovery-based metabolomics and proteomics analyses were performed on tissue samples collected immediately post-treatment (d 1). Data (except for omics) were analyzed using ProcMixed of SAS 9.4 with the fixed effect of treatment and steer as the experimental unit. Omics data were analyzed using MetaboAnalyst; metabolites and proteins of interest were identified based on a fold change threshold of 1.20 and t-test P-value of 0.10. On d 1, percent of pretreatment BW and DM intake were least for TRANS steers (P ≤ 0.06). Percent of pretreatment BW remained lesser for TRANS steers on d 8 (P = 0.05). Serum haptoglobin was greatest for TRANS steers immediately post-treatment (P = 0.02). Additionally, TRANS steers exhibited the greatest increase in the neutrophil to lymphocyte ratio and serum non-esterified fatty acids during the treatment period (P < 0.01), indicating TRANS steers experienced a more robust inflammatory and neuroendocrine response. Immediately post-treatment, liver superoxide dismutase activity tended to be greatest for both DEPR and TRANS (P = 0.07) while muscle superoxide dismutase activity was only greatest for TRANS (P = 0.02), suggesting TRANS steers may have experienced more oxidative stress due to the additional physical effort required to stand and maintain balance during transit. The abundance of several proteins (alpha-2-HS-glycoprotein) and metabolites (lactate, citrate, tri-hydroxybutyric acid, and leucine) associated with energy metabolism were altered in the liver and muscle of TRANS. The differential responses for DEPR versus TRANS steers indicate muscle plays an important role in how cattle respond to and recover from transportation stress.

Effects of physical preparation of diets and inclusion rate of modified wet distillers grains with solubles on production and rumen measurements of lactating dairy cows.

Our objective was to determine if methods for preparing total mixed ration [TMR; horizontal paddle mixer with knives (PK) vs. vertical auger (VA) mixer] would alter the physical form of the TMR and affect utilization of diets with increasing amounts of modified wet distillers grains with solubles (MWDGS). Holstein cows (n = 24 with 12 ruminally cannulated; 144 d in milk ± 31 d at start) were used in a split-plot design with mixer type as the whole plot and MWDGS concentrations as subplots in a replicated 3 × 3 Latin square arrangement with 35-d periods. Inclusion rates of MWDGS were 10, 20, and 30% of dietary dry matter, primarily replacing corn, soybean meal, soyhulls, and whole cottonseed. Feed dry matter intake (DMI) was less for PK (23.8 kg/d) than for VA (25.7 kg/d), but was unaffected by MWDGS concentration. Milk production did not differ by concentration of MWDGS or by interaction of MWDGS × mixer. Milk fat percentage declined with increasing MWDGS but the interaction between mixer and MWDGS showed that decreases were larger with VA mixing. Cows fed the diet containing 30% MWDGS mixed with PK averaged 3.45% (1.24 kg/d) milk fat, whereas cows fed the same diet mixed with VA averaged 2.81% (1.10 kg/d) fat. Concentrations of trans-10,cis-12 C18:2 in milk fat likely explain the differences observed in milk fat yield; the concentration of trans-10,cis-12 C18:2 increased as MWDGS was increased and the MWDGS × mixer interaction showed that VA had greater concentrations. Greater mean particle size and increased variation in particle size with VA may partially explain differences in milk fat via increased sorting that allowed for an altered rumen environment and favored alternative biohydrogenation pathways. Feed conversion efficiency (FCE; energy-corrected milk/DMI) decreased linearly as MWDGS increased, but FCE tended to be maintained when higher MWDGS diets were mixed using PK rather than VA. Ruminal pH and ammonia concentration decreased linearly as MWDGS increased. The PK mixer resulted in greater FCE when higher amounts of MWDGS were fed, primarily because milk fat content and yield were not as depressed and DMI was lower at similar milk yields.

Pre-transit vitamin C injection improves post-transit performance of beef steers.

Although cattle can synthesize vitamin C (VC) endogenously, stress may increase VC requirements above the biosynthetic threshold and warrant supplementation. This study investigated the effects of a VC injection delivered before or after a long-distance transit event on blood parameters and feedlot performance of beef steers. Fifty-two days prior to trial initiation, 90 newly weaned, Angus-based steers from a single source were transported to Ames, IA, USA. On day 0, 72 steers (356 ± 17 kg) were blocked by BW and randomly assigned to intramuscular injection treatments (24 steers/treatment): saline injection pre- and post-transit (CON), VC (Vet One, Boise, ID, USA; 5 g sodium ascorbate/steer) injection pre-transit and saline injection post-transit (PRE) or saline injection pre-transit and VC injection post-transit (POST). Following pre-transit treatment injections, steers were transported on a commercial livestock trailer for approximately 18 h (1675 km). Post-transit (day 1), steers were sorted into pens with one GrowSafe bunk/pen (4 pens/treatment; 6 steers/pen). Steers were weighed on day 0, 1, 7, 30, 31, 56 and 57. Blood was collected from 3 steers/pen on day 0, 1, 2 and 7; liver biopsies were performed on the same 3 steers/pen on day 2. Data were analyzed as a randomized complete block design (experimental unit = steer; fixed effects = treatment and block) and blood parameters were analyzed as repeated measures. A pre-transit VC injection improved steer average daily gain from day 7 to 31 (P = 0.05) and overall (day 1 to 57; P = 0.02), resulting in greater BW for PRE-steers on day 30/31 (P = 0.03) and a tendency for greater final BW (day 56/57; P = 0.07). Steers that received VC pre- or post-transit had greater DM intake from day 31 to 57 (P = 0.01) and overall (P = 0.02) v. CON-steers. Plasma ascorbate concentrations were greatest for PRE-steers on day 1 and POST-steers on day 2 (treatment × day; P < 0.01). No interaction or treatment effects were observed for other blood parameters (P ≥ 0.21). Plasma ferric-reducing antioxidant potential and malondialdehyde concentrations decreased post-transit (day; P < 0.01), while serum non-esterified fatty acids and haptoglobin concentrations increased post-transit (day; P < 0.01). In general, blood parameters returned to pre-transit values by day 7. Pre-transit administration of injectable VC to beef steers mitigated the decline in plasma ascorbate concentrations and resulted in superior feedlot performance compared to post-transit administration.

Effect of trace mineral source on mineral status and performance of beef steers fed low- or high-sulfur diets.

A 2 × 2 factorial assessed the effect of trace mineral (TM) sources fed within low- or high-S diets on the mineral status and performance of cattle. Angus crossbred steers ( = 48; 6/pen) were blocked by BW (316 ± 16.6 kg), assigned to low-S (0.27%; LS) or high-S (0.54%; HS; added as CaSO) diets, and supplemented TM at 10 mg Cu, 30 mg Zn, and 20 mg Mn/kg DM from hydroxy (HYD; IntelliBond; Micronutrients USA LLC, Indianapolis, IN) or inorganic (sulfates; ING) sources ( = 12 steers/treatment). Steers were fed corn silage and corn-based diets via GrowSafe bunks in the growing period (GP; 84 d) and finishing period (FP; 77 d), respectively. Plasma and liver were collected at trial initiation and end of GP and FP for mineral concentrations. End of GP and FP red blood cell lysate superoxide dismutase (SOD) and Mn-SOD activity and liver glutathione concentrations were measured. Data were used as a 2 × 2 factorial using Proc Mixed of SAS (SAS Inst. Inc., Cary, NC) with initial plasma and liver status analyzed as covariates. High S decreased ( < 0.01) liver Cu and tended ( ≤ 0.10) to decrease plasma Cu concentrations. Liver Cu concentrations were lower in HYD than ING in the FP ( < 0.01). High S decreased ( = 0.04) GP plasma Zn concentrations and tended to decrease ( = 0.10) GP liver Zn. There were GP ( = 0.05) and FP ( = 0.02) S × TM effects for liver Mn concentrations where GP LS-HYD was greater than all other treatments, whereas FP LS-HYD was lower than HS-HYD and LS-ING and FP HS-ING was less than LS-ING. Glutathione, SOD, and Mn-SOD were not different ( ≥ 0.13) in the GP, but S × TM tended to affect FP Mn-SOD ( = 0.10), where LS-HYD tended to be lower than LS-ING. Oxidized glutathione in FP tended to be lower ( = 0.06) for HYD than ING. In the GP, there were S × TM effects on performance where LS-HYD had greater ADG and G:F ( ≤ 0.05) than HS-HYD, whereas LS and HS-ING were intermediate. For FP performance S × TM effects were noted where LS-HYD and HS-ING tended ( = 0.10) to gain more than HS-HYD and HS-HYD had lower G:F ( = 0.04) than HS-ING. There were no effects of S × TM on final BW, DMI, or ADG ( ≥ 0.11); however, HS-HYD had lower G:F than other treatments overall ( = 0.05). High S decreased back fat and yield grade ( = 0.03), and rib eye area was smaller for HYD than ING ( = 0.02). In this study HS decreased markers of Cu and Zn status, and differential effects of HYD vs. ING minerals were noted across dietary phases, although all steers maintained adequate TM status.

Effect of post-ethanol extraction sorghum silage as a forage source in growing and finishing diets on steer performance, carcass characteristics, and nutrient digestibility.

Two experiments evaluated the use of post-ethanol extraction sorghum silage as an alternative forage source in feedlot diets. Seventy-two crossbred steers (397 kg [SD 23]) were used to evaluate growth and carcass characteristics. Steers were blocked by BW into pens of 6 steers and randomly assigned to growing diets containing 40% (DM basis) sorghum silage (SS; 57.6% NDF) or grass hay (CON; 63.3% NDF) for 56 d ( = 6 pens per treatment). Within each treatment, steers transitioned to dry-rolled corn-based finishing diets (fed for 56 d) containing 6% effective NDF contributed by the forage source, resulting in forage inclusions of 16% for SS and 13.1% for CON, where forage replaced corn. A subsample of steers ( = 12 per treatment) housed in pens equipped with GrowSafe bunks were used for determination of growing phase diet total tract digestibility. From d 28 to 42, steers received titanium dioxide at approximately 10 g∙steer∙d, and fecal samples were collected on d 41 and 42. Fecal and total mixed ration samples were dried and ground for analysis of DM, OM, NDF, ADF, CP, ether extract (EE), and starch. Data were analyzed with the MIXED procedure of SAS, with fixed effects of treatment and block for performance and carcass data or treatment for digestibility data; significance was determined at ≤ 0.05 and tendencies at ≤ 0.10. Growing phase DMI and ADG did not differ due to treatment ( ≥ 0.19); however, SS-fed steers had improved G:F compared with CON-fed steers ( = 0.04). Finishing period ADG and G:F did not differ ( ≥ 0.15) between treatments, despite SS-fed steers having lesser ( = 0.008) DMI than CON-fed steers. No differences in DMI, ADG, or G:F over the whole trial were noted between treatments ( ≥ 0.12) nor were any carcass traits affected ( ≥ 0.23). During the digestibility assessment period, DMI was less ( = 0.003) in SS-fed steers. Growing phase total tract apparent digestibility of DM and starch did not differ ( ≥ 0.19) due to treatment; however, OM digestibility tended to be greater ( = 0.09) in SS-fed steers. Steers fed SS had greater ( ≤ 0.03) digestibility of EE, CP, NDF, and hemicellulose than CON-fed steers. However, CON-fed steers had greater ( < 0.0001) ADF digestibility than SS-fed steers. These data suggest that postextraction sorghum silage can be effectively used in feedlot diets as an alternative forage source.

Influence of feed efficiency classification and growing and finishing diet type on meat tenderness attributes of beef steers.

Enhancing feed efficiency (FE) in cattle continues to be an important goal in the beef industry. Previous research suggests improving FE may alter calpain system activity, potentially having negative effects on meat tenderness. The objective of this study was to assess the potential influence of beef cattle FE on postmortem meat tenderness. During the growing phase, 181 crossbred steers were fed for 76 d at the University of Missouri on a whole shell corn-based diet (MU-Corn; = 90) or a roughage-based diet (MU-Rough; = 91). Within diet, steers were classified for FE based on residual feed intake (RFI) calculations. Within each growing phase diet, the 12 most feed efficient (HFE; average RFI -3.33 ± 0.77) and 12 least feed efficient (LFE; average RFI 2.90 ± 0.94) steers (48 steers total) were selected and shipped to Iowa State University for the finishing phase. Steers were fed in pens with GrowSafe bunks and equally assigned to a cracked corn-based finishing diet (ISU-Corn) or a byproduct-based finishing diet (ISU-Byp) for the 87-d finishing phase. After a 24-h chill, rib sections were collected from all carcasses and aged for 2 or 14 d prior to analysis of calpastatin activity and calpain 1 autolysis (d 2), troponin-T degradation (d 2 and 14), proximate composition (d 2), and Warner-Bratzler shear force (WBSF; d 14). Data were analyzed as a 2 × 2 × 2 factorial, with steer or steak as the experimental unit ( = 6 per treatment combination). There were no 3- or 2-way interactions ( ≥ 0.12) noted for any data, with the exception of steak lipid content, which tended ( = 0.08) to be affected by FE × Iowa State University diet. Steaks from LFE steers had greater lipid content compared with steaks from HFE steers within ISU-Byp, with no differences due to FE within ISU-Corn. Growing diet did not affect calpastatin activity, calpain 1 autolysis, or troponin T degradation ( ≥ 0.12); however, MU-Rough had greater WBSF than MU-Corn ( = 0.05). Day-2 calpastatin activity tended ( = 0.10) to be greater in steaks from HFE vs. LFE steers, although no differences due to FE classification were observed for calpain 1 protein, troponin-T degradation, or WBSF ( ≥ 0.13). Finishing diet did not affect calpastatin activity, calpain 1 autolysis, d-2 troponin-T degradation, or WBSF ( ≥ 0.24); however, d-14 troponin-T degradation was greater in ISU-Corn than in ISU-Byp ( = 0.005). In this study, using phenotypic extremes for FE revealed a tendency for greater calpastatin activity only in highly feed-efficient steers; however, these data indicate that high-fiber diets may negatively impact meat tenderness, and further work is needed to clarify the influence of diet type on parameters of meat tenderness.

Immunometabolic parameters in overweight dogs during weight loss with or without an exercise program.

The influence of physical activity on metabolic health in overweight dogs is unknown. This study was conducted to evaluate biomarkers of immunometabolic health in relation to changes in physical activity and adiposity. Client-owned overweight dogs participated in a 12-wk intervention based on caloric restriction combined with a training program (fitness and diet [FD] group, n = 8), or caloric restriction alone (diet-only [DO] group, n = 8). Physical activity was monitored by accelerometry. All dogs were fed the same diet and achieved similar weight loss. Fasting blood samples were collected before and after 6- and 12-wk intervention. Insulin resistance was evaluated from plasma insulin and C-peptide as well as homeostasis model assessment. Inflammation and dyslipidemia were evaluated from circulating leptin, adiponectin, C-reactive protein (CRP), monocyte chemoattractant factor-1 (MCP-1), interleukin-8 (IL-8), and cholesterol. Accelerometer counts in both groups were high compared with previous reports of physical activity in overweight dogs. No difference in blood parameters was evident between groups, evaluated by linear mixed-effects model (P > 0.05). Within the groups, the following changes were significant by t-test (P < 0.05): leptin decreased in both groups. Within the FD group, IL-8, MCP-1, and CRP decreased at 6 wk and IL-8 and cholesterol at 12 wk. Within the DO group, C-peptide and HOMA decreased at 6 wk and C-peptide at 12 wk. We conclude that, for both groups, weight loss resulted in minor indications of improved immunometabolic health, whereas this level of physical activity did not add further benefits.

The influence of supplemental Zn-amino acid complex and ractopamine hydrochloride feeding duration on growth performance and carcass characteristics of finishing beef cattle.

Previous research indicates that finishing steer ADG and G:F increases linearly with increasing dietary Zn-amino acid complex (ZnAA) supplementation in cattle fed ractopamine hydrochloride (RAC). The objective of this study was to determine the influence of supplemental Zn-amino acid complex on growth performance and carcass characteristics of finishing steers fed RAC for 0, 28, or 42 d prior to harvest. This study was organized as 2 groups (GRP) of steers fed concurrently, for 91 (GRP 1) or 84 d (GRP 2). A total of 324 steers (463 ± 23.4 kg) were fed a corn-based finishing diet supplemented with 60 mg Zn/kg diet DM (as ZnSO). Steers were blocked by weight to pens of 6 steers, and assigned to receive either 0 (CON) or 60 mg supplemental Zn/kg DM from ZnAA ( = 27 pens per treatment). Receiving ZnAA for 49 (GRP 1) or 42 d (GRP 2) prior to start of RAC feeding had no impact on growth of steers ≥ 0.19). Forty-two d prior to harvest, pens were equally assigned within CON or ZnAA treatments to receive RAC at 300 mg∙steer∙d for 0 (NoRAC), 28 (28RAC), or 42 d (42RAC) prior to harvest, creating 6 final treatments ( = 9 pens per treatment). All steers within a GRP were harvested on the same day. Pen was the experimental unit, and the statistical model included the fixed effects of ZnAA, RAC, and block nested within GRP, and the random effect of pen. Ractopamine hydrochloride supplementation increased carcass-adjusted ADG, final BW, HCW, and ribeye area ( ≤ 0.007). There was an effect of ZnAA within 28RAC and 42RAC where carcass-adjusted ADG ( ≤ 0.10), and final BW and HCW ( ≤ 0.05) were greater in ZnAA supplemented vs. CON steers, and 28RAC steers supplemented with ZnAA had improved overall carcass-adjusted G:F relative to CON steers ( = 0.04). However, when steers did not receive RAC there was no effect of ZnAA on final BW, ADG, or HCW ( ≥ 0.78). Additionally, ZnAA supplementation had no effect on the difference in performance between steers supplemented with RAC for 28 vs. 42 d ( 0.21). In conclusion, under the conditions of this study supplemental ZnAA did not prevent the diminished response to RAC as days on RAC increased from 28 to 42. However, there appears to be a synergistic effect of ZnAA on RAC-induced cattle growth, as supplementing 60 mg Zn/kg DM from ZnAA to cattle fed RAC improved overall growth and HCW.

Effect of ferric ammonium citrate in feedlot diets with varying dried distillers' grains inclusion on ruminal hydrogen sulfide concentrations and steer growth.

Angus-cross steers ( = 128) were used to examine the effects of supplementing ferric ammonium citrate (FAC; 300 mg ferric Fe/kg DM) to diets of 20, 40, or 60% dried distillers' grains plus solubles (DDGS) on growth performance, liver mineral and ruminal hydrogen sulfide (HS) concentrations, and carcass traits of finishing steers. Steers were blocked by initial BW (436 ± 10.6 kg) into pens of 4 and randomly assigned to 1 of 6 treatments ( = 5 or 6 pens per treatment) including a 20, 40, or 60% DDGS inclusion diet with (+) or without (-) 300 mg Fe/kg DM from FAC. Liver biopsies (d -9/-10 and 96) and HS measures (d 0, 7, 14, 21, and 95) were determined from 1 steer/pen. Steers were harvested on d 102 and carcass data were collected. A treatment × month effect ( ≤ 0.006) was noted for ADG and G:F, in which the 20-FAC ADG and feed efficiency were greater ( ≤ 0.02) between d 0 to 28 but lesser ( ≤ 0.04) from d 29 to 56 than that of the 20+FAC steers. Final BW linearly decreased ( < 0.01) as DDGS inclusion increased. Final BW tended to be greater ( = 0.10) in the 60+FAC steers than in the 60-FAC steers, whereas final BW was not different ( ≥ 0.32) due to FAC supplementation in the 20 or 40% DDGS diets. A quadratic effect was noted for DMI ( = 0.02), where 60% DDGS decreased DMI. Within the 20% DDGS diet FAC+ improved DMI ( = 0.03) but had no effect within 40 or 60% DDGS inclusion. Ruminal HS concentrations were not affected ( ≥ 0.25) by FAC, but increasing DDGS linearly increased ( < 0.01) ruminal HS values. Liver Cu was decreased ( < 0.01) by FAC across all DDGS inclusions and tended to linearly decrease ( = 0.06) with increasing DDGS inclusion, whereas liver Fe, Mn, and Zn were not altered ( ≥ 0.11) by DDGS inclusion. Liver Zn concentrations tended to be ( = 0.08) or were ( = 0.03) decreased by FAC supplementation within 20 and 40% DDGS, respectively. Increasing the inclusion of DDGS linearly decreased ( = 0.04) HCW and quadratically affected marbling score where the 40% DDGS had the greatest ( = 0.02) marbling scores. Supplementation of FAC within 60% DDGS improved ( ≤ 0.03) HCW and LM area. Marbling scores were greater ( ≤ 0.04) in 20+FAC and 40+FAC compared with 20-FAC and 40-FAC, respectively. In conclusion, although ruminal HS concentrations were not affected by FAC under the conditions of this study, supplementing FAC to diets containing 60% DDGS improved HCW and LM area, suggesting that FAC may be beneficial when dietary S concentrations exceed 0.5%.

The effects of increasing supplementation of zinc-amino acid complex on growth performance, carcass characteristics, and inflammatory response of beef cattle fed ractopamine hydrochloride.

Forty-two Angus crossbred steers (380 ± 5.3 kg) were enrolled in a finishing study to evaluate the influence of a supplemental Zn amino-acid complex (ZnAA; Availa-Zn) on performance and carcass characteristics of finishing steers in combination with ractopamine hydrochloride (RAC). Steers were stratified by BW into 7 pens of 6 steers each, and individual feed intake was measured. Steers were assigned to 1 of 4 treatments for 86 d (pre-RAC period): a dry-rolled corn-based diet supplemented with 60 mg Zn/kg DM from ZnSO and no supplemental ZnAA (CON; analyzed 88 mg Zn/kg DM; = 6) or CON diet supplemented with 30 (Zn30; = 12), 60 (Zn60; = 12), or 90 (Zn90; = 11) mg Zn/kg DM from ZnAA. Day 86 BW and G:F displayed a quadratic tendency ( = 0.09) with Zn60 steers being greater than the other treatments. Plasma cyclic adenosine monophosphate tended to linearly increase with increasing ZnAA ( = 0.10). On d 88, 6 of 12 steers (one of the 2 pens) receiving supplemental ZnAA was randomly selected to be supplemented with RAC at 300 mg∙steer∙d for the final 28 d of the experiment (RAC period). This created 7 final treatments: CON: no supplemental ZnAA, no RAC ( = 5); Zn30: Zn30, no RAC ( = 5); Zn30R: Zn30 + RAC ( = 6); Zn60: Zn60, no RAC ( = 6); Zn60R: Zn60 + RAC ( = 6); Zn90: Zn90, no RAC ( = 5); and Zn90R: Zn90 + RAC ( = 6). During the RAC period, as supplemental ZnAA increased within RAC-supplemented treatments, there was a linear increase in final BW, ADG, and G:F ( < 0.05). However, there was no effect of supplemental ZnAA on BW, ADG, or G:F during this period in non-RAC fed steers ( ≥ 0.44). Day 111 plasma Cu was increased, plasma Fe decreased, and leukocyte counts and serum interleukin-8 concentrations were greater ( < 0.05) in RAC-fed steers suggesting that RAC may elicit a mild inflammatory response. There was a tendency for increasing Zn supplementation to decrease plasma haptoglobin within RAC-fed steers ( = 0.07), suggesting that Zn may alter the inflammatory response. Overall, Zn60 improved growth performance during the pre-RAC period. Zinc supplemented as ZnAA appears to improve growth in combination with RAC supplementation, suggesting that Zn may enhance or support the biological function of RAC. Additionally, these results indicate that feeding RAC impacts trace mineral status, and potentially causes a non-specific inflammatory response, but further research is required to define this response.

Influence of growing phase feed efficiency classification on finishing phase growth performance and carcass characteristics of beef steers fed different diet types.

A 5-yr study was conducted using 985 crossbred steers (464 kg [SD 32]) fed in 6 separate, replicated groups to determine the influence of growing phase (GP) feed efficiency (FE) classification and diet type on finishing phase (FP) FE of steers. During the GP at the University of Missouri, steers were fed either a whole shell corn-based diet (G-Corn; 528 steers) or a roughage-based diet (G-Rough; 457 steers) using GrowSafe feed bunks to measure DMI for 69 to 89 d. At the end of the GP, steers were ranked by residual feed intake (RFI) within diet, shipped to Iowa State University, and blocked into FP pens (5 to 6 steers/pen) by GP diet and RFI rank (upper, middle, or lower one-third). Steers were transitioned to either FP cracked corn- or byproduct-based diets and fed until 1.27 cm backfat was reached. After completion of the sixth group, average GP G:F within GP diet was calculated for each FP pen (168 total pens) using GP initial BW as a covariate (G-Corn: 0.207 [SD 0.038]; G-Rough: 0.185 [SD 0.036]). Pens were classified as highly feed efficient (HFE; >0.5 SD from the G:F mean; 58 pens), mid feed efficient (MFE; ±0.5 SD from the G:F mean; 60 pens), or lowly feed efficient (LFE; <0.5 SD from the G:F mean; 50 pens). Data were analyzed using PROC MIXED of SAS. Experimental unit was FP pen and the model included the fixed effects of GP diet, FE classification, FP diet, and the interactions. Group (1 to 6) was included as a fixed effect. There were no 3-way interactions ( ≥ 0.2) for any measured traits. Finishing phase G:F was not affected by any interactions ( ≥ 0.5) but was greater ( ≤ 0.03) for HFE versus MFE and LFE and greater ( = 0.02) for MFE versus LFE. Growing phase diet × FE classification effects were detected ( ≤ 0.01) for FP final BW (FBW), ADG, and DMI. Among G-Rough steers, HFE and MFE had greater ( ≤ 0.04) FBW and ADG than LFE, but among G-Corn steers, LFE had heavier ( = 0.03) FBW than HFE whereas ADG was unaffected ( ≥ 0.2) by FE classification. Dry matter intake was unaffected ( ≥ 0.3) by FE classification among G-Rough steers, but among G-Corn steers, LFE had greater ( ≤ 0.003) DMI than MFE and HFE. Overall, differences in FP G:F between FE classifications were driven by different factors depending on diet; ADG differed among roughage-grown steers and DMI differed among corn-grown steers. Ultimately, steers classified as HFE during the GP still had superior FE during the FP.

Relationship between antioxidant capacity, oxidative stress, and feed efficiency in beef steers.

Feed efficiency (FE) can vary between individuals but sources of variation are not well characterized. Oxidative stress is among the biological mechanisms believed to contribute to variation. The objective of this study was to evaluate the relationship between FE, antioxidant activity, and oxidative stress in feedlot steers representing phenotypic extremes for FE. Crossbred beef steers ( = 181) fed 70-d growing phase (GP) whole-shell corn-based (G-Corn) or rye baleage and soybean hull-based (G-Rough) diets in GrowSafe bunks at the University of Missouri were shipped to Iowa State University where the 12 most feed efficient (HFE) and 12 least feed efficient (LFE) steers from each diet (n = 48; 467 kg [SD 51]) were selected for evaluation. Steers received diets similar to GP diets, and 3 d after arrival, blood was sampled to evaluate antioxidant activity and oxidative stress markers for the GP following transit. Steers were transitioned to finishing phase (FP) cracked corn-based (F-Corn) or dried distillers' grains and soybean hull-based (F-Byp) diets, and on FP d 97, blood samples for the FP were collected. Data for the GP were analyzed as a 2 × 2 factorial, and data for the FP were analyzed as a 2 × 2 × 2 factorial using PROC MIXED of SAS. No GP diet × FP diet, FP diet × FE group, or 3-way interactions were noted ( ≥ 0.11) for FP measures. Steers fed the G-Rough diet had greater ( = 0.04) GP plasma protein carbonyl concentrations. During the GP, HFE steers had greater ( ≤ 0.04) protein carbonyl and ratio of oxidized:reduced blood lysate glutathione concentrations than LFE steers. There were GP diet × FE group interactions ( ≤ 0.03) during the GP and FP. During the GP, total blood lysate superoxide dismutase (SOD) activity was greater ( ≤ 0.03) in G-Rough/LFE steers than in G-Rough/HFE and G-Corn/LFE steers; G-Corn/HFE steers were intermediate. The G-Rough/LFE steers had greater ( < 0.04) glutathione peroxidase (GPX) activity than other groups and greater ( = 0.03) plasma malondialdehyde concentrations than G-Corn/LFE steers. During the FP, the G-Rough/LFE steers had greater ( ≤ 0.04) GPX activity than G-Rough/HFE and G-Corn/LFE steers; G-Corn/HFE steers were intermediate. The F-Byp diet had greater ( < 0.01) protein carbonyl than the F-Corn diet, and no other FP diet effects were noted ( ≥ 0.3) for any FP measures. The GP diet and FE groups had stronger relationships with antioxidant activity and oxidative stress markers measured for the GP than for the FP. Overall, antioxidant activity may play a role in FE as LFE steers, driven largely by G-Rough/LFE steers, had greater SOD activity and GPX activity than HFE steers, potentially using a greater proportion of energy otherwise available for tissue accretion.

Influence of feed efficiency classification on diet digestibility and growth performance of beef steers.

The diet digestibility and feed efficiency (FE) relationship is not well characterized in cattle. The study objective was to determine effects of growing phase FE and diet as well as finishing phase diet on diet digestibility and finishing phase FE. Two groups, totaling 373 crossbred steers, were fed for 70 d at the University of Missouri for the growing phase and then shipped to Iowa State University (ISU) for finishing. GrowSafe feed bunks were used during both the growing and the finishing phases. Steers were fed either growing phase whole shell corn (G-Corn) or growing phase roughage-based (G-Rough) diets. Within each group, the 12 greatest and 12 least feed efficient steers from each growing diet ( = 96 total; 48 steers/group; 488 ± 5 kg) were selected for further evaluation. At ISU, steers were fed an average of 10 g TiO/steer daily in receiving phase diets similar to growing diets for 15 d, with fecal grab samples collected on d 14 and 15 to determine diet DM digestibility during receiving (GDMdig). For finishing, steers were transitioned to byproduct-based diets (F-Byp) or corn-based diets (F-Corn) with 12 steers per growing-finishing diet combination per group. Optaflexx (200 mg/d) was fed for 28 d before harvest, and the TiO protocol was repeated immediately before introducing Optaflexx to determine diet DM digestibility during finishing (FDMdig). Data from the 2 groups (96 steers) were pooled, and steers were ranked by growing phase G:F and then classified as the 24 greatest feed efficient (HFE) or 24 least feed efficient (LFE) steers from each growing diet. Data were analyzed using PROC MIXED of SAS with group applied as a fixed effect. There was a positive correlation between GDMdig and FDMdig for steers fed nutritionally similar diets during both feeding phases, G-Rough/F-Byp steers ( = 0.68, < 0.01) and G-Corn/F-Corn steers ( = 0.49, = 0.02), but a negative correlation for G:F between phases in G-Rough/F-Corn steers ( = -0.57, < 0.01). Finishing G:F was greater in HFE steers versus LFE steers ( = 0.04), but there was no difference ( ≥ 0.5) in GDMdig or FDMdig due to FE classification. There was a positive correlation for DM digestibility between feeding phases when steers were grown and finished on similar diets. Overall, FE was repeatable but was negatively correlated between phases when steers were roughage grown and corn finished, reinforcing the idea that cattle should be FE tested using diet types similar to the production environment of interest.

Effects of increased inclusion of algae meal on finishing steer performance and carcass characteristics.

The deoiled residue from the production of heterotrophic microalgae can be combined with soyhulls to form a novel feedstuff called algae meal (ALG). To determine the effects of replacing corn in a finishing diet with ALG on growth, mineral status, carcass characteristics, and longissimus thoracis fatty acid profile, crossbred steers (168) were blocked by BW (432 ± 30.8 kg) into pens of 6 steers (7 pens per treatment). Pens received 1 of 4 diets: a corn-based control (CON), 14% ALG, 28% ALG (ALG28), and 42% ALG (ALG42). Corn was replaced by ALG on a DM basis. Steer BW were taken on d 0, 1, 28, 56, 74, 101, and 102, and steers were harvested on d 103. Pen was the experimental unit and DMI, ADG, and G:F data were analyzed as repeated measures. Two steers per pen were selected for sampling of blood and liver (d -1 and 96) and collection of rib steaks at harvest. There was a treatment by × time effect ( = 0.10) for overall DMI, where DMI linearly increased ( ≤ 0.008) across all time periods except Day 28 through, 56 when DMI was not different between ALG28 and ALG42. There was a treatment by time effect for ADG ( < 0.01), with ADG linearly decreasing ( ≤ 0.03) in the first and third month, not differing ( = 0.95) in the second month, and linearly increasing ( < 0.01) in the fourth month as ALG increased in the diet. Final BW and HCW did not differ ( ≥ 0.50) between CON- and ALG-fed cattle. There was a treatment × time effect for G:F ( < 0.01), with G:F linearly decreasing ( ≤ 0.01) in the first 3 mo as ALG increased in the diet, whereas G:F linearly increased ( < 0.01) in the fourth month. Based on steer performance, calculated dietary NEg linearly decreased ( < 0.01) as ALG increased in the diet. Yield grade linearly decreased ( = 0.02) and there was a tendency for dressing percent and 12th-rib back fat to linearly decrease ( ≤ 0.10) as ALG increased in the diet. Plasma Cu, Fe, and Mg concentrations were not different ( ≥ 0.31) in CON vs. ALG cattle; however, plasma Zn concentrations linearly increased ( = 0.03) as ALG increased in the diet. Total lipid, SFA, MUFA, and PUFA concentrations in the longissimus thoracis did not differ ( ≥ 0.13) between CON- and ALG-fed cattle. Interestingly, the atherogenic index linearly decreased ( < 0.01) as ALG increased in the diet. Algae meal may have a lesser energy value than corn; however, a minimal effect on carcass performance suggests ALG may serve as a potential replacement for corn in feedlot diets.

Assessment of algae meal as a ruminant feedstuff: Nutrient digestibility in sheep as a model species.

Heterotrophic microalgae combined with soyhulls forms an algae meal (ALG), which contains partially deoiled microalgae (PDM; 57% DM basis) and soyhulls (43%). Two experiments were conducted to evaluate the effects of PDM and ALG on lamb digestibility. In Exp. 1, 8 wethers (23.02 ± 0.54 kg) were used in a 4 × 4 Latin square design to determine the effect of the PDM portion of ALG on total tract nutrient digestibility. Diets included a soyhull-based control (CON; 53% soyhulls), 10% PDM from ALG, 20% PDM from ALG (PDM20), and 30% PDM from ALG. Dry matter and OM intake and fecal DM and OM output were similar ( ≥ 0.11) between CON- and ALG-fed lambs. Urine output linearly increased ( = 0.02) as PDM increased in diets. Dry matter, OM, NDF, and ADF digestibility linearly decreased ( < 0.01) as PDM increased in diets. Ether extract digestibility did not differ ( = 0.24) between CON- and PDM-fed lambs. Nitrogen digestibility and N retention linearly decreased ( ≤ 0.05) as PDM increased in the diet. In Exp. 2, to determine the effects of ALG on diet and nutrient digestibility and N retention, 10 whiteface cross wethers (33.71 ± 0.55 kg) were used in a replicated 5 × 5 Latin square. Diets included a cracked corn-based control (CORN), 15% ALG, 30% ALG, 45% ALG (ALG45), and 60% ALG (ALG60). Dry matter and OM digestibility linearly ( < 0.001) decreased as ALG inclusion increased. Digestibility of NDF and ADF were lesser ( ≤ 0.03) for CORN-fed sheep than for ALG-fed sheep and linearly ( ≤ 0.03) increased as ALG increased in the diet. Ether extract digestibility was lesser ( = 0.002) for CORN than ALG, with a linear ( = 0.002) increase as ALG inclusion increased. There was a cubic ( = 0.03) effect for N digestibility with ALG45 and ALG60 being lesser and CORN being greater than all other treatments. Retention of N and plasma urea N concentration did not differ ( ≥ 0.22) between CORN and ALG. Nonfibrous carbohydrate digestibility linearly ( < 0.001) decreased as ALG increased in the diet. These results suggest that the PDM portion of ALG may be less digestible than soyhulls in ruminants, and differences in N retention in Exp. 1 may suggest an effect on growth in lambs. Furthermore, changes in digestibility of specific nutrients suggest that ALG is more characteristic of a concentrate rather than a fibrous feedstuff. However, lambs will readily consume ALG and this novel feedstuff could potentially serve as a viable component of ruminant diets.

Determining the preference, in vitro digestibility, in situ disappearance, and grower period performance of steers fed a novel algae meal derived from heterotrophic microalgae.

A series of 3 experiments were conducted to determine the in vitro and in situ disappearance, preference, and performance of steers consuming increasing concentrations of a novel feedstuff called algae meal (ALG). In Exp. 1 and 2, 3 ruminally cannulated steers (998 ± 103 kg BW) and 4 treatments were utilized: a dry-rolled corn-based diet (CON), or 15% ALG (A15), 30% ALG (A30), or 45% ALG (A45) as a DM replacement of corn. Experiment 1 was performed as a 3 × 6 unbalanced Latin square with six 5-d periods, and 6 paired diet combinations to determine steer preference, based on DMI for 4 h after feed delivery. Experiment 2 was conducted as a 3 × 4 unbalanced Latin square with 3 diets fed each period and four 14-d periods to determine in situ disappearance of ALG, soyhulls, corn, and hay as ALG increased in the diet. Experiment 3 utilized 48 individually fed steers (292 ± 22.4 kg; n = 12) in a 55-d grower trial, stratified by BW into 1 of 4 dietary treatments: a control wet corn gluten feed-based diet with no ALG (ALG0), or 15% ALG (ALG15), 30% ALG (ALG30), or 45% ALG (ALG45) in place of wet corn gluten feed on a DM basis. Digestibility of ALG was greater (P < 0.001) than soyhulls at 24 h, but was similar (P = 0.82) at 48 h, with hay being least (P < 0.001) digestible at 24 and 48 h. In Exp. 1, the proportion of total DM consumed by steers during the 4-h postfeeding was affected (P = 0.01) by paired diet combination. Steers preferred A15 or A30 diets over the A45 diet (P ≤ 0.008); however, no difference in preference between CON and A45 was noted (P = 0.76). Steers tended (P = 0.09) to prefer A30 over CON, but A15 and CON were not different (P = 0.75). No other paired diet combinations altered (P ≥ 0.23) DMI. In Exp. 2, inclusion of ALG in the diet did not affect (P ≥ 0.12) rate of, or overall in situ disappearance of, corn, soyhulls, or ALG. In Exp. 3, ADG tended ( = 0.06) to increase as ALG increased in the diet. There was a linear increase ( < 0.001) in DMI as ALG increased in the diet, and as a result, G:F linearly decreased ( = 0.01). In summary, ready consumption of the novel algae meal by steers and support of growth in steers at up to 45% of the diet suggest the algae meal is a viable feedstuff for ruminants.

Influence of distillers grains resulting from a cellulosic ethanol process utilizing corn kernel fiber on nutrient digestibility of lambs and steer feedlot performance.

Two experiments evaluated the effects on animal performance of traditional wet distillers grains (T-WDG) compared to cellulosic wet distillers grains (C-WDG) from a new process converting corn kernel fiber into cellulosic ethanol. The resulting coproduct has greater CP and decreased starch and ether extract (EE) concentrations (34.0% CP, 1.6% starch, 7.3% EE) compared to T-WDG (32.5% CP, 5.1% starch, 7.7% EE). In Exp. 1, 10 wethers (34.1 ± 2.35 kg, SD) were used in a replicated 5 × 5 Latin square to evaluate digestibility of DM, fiber, EE, and N. Diets including a corn-based control with 7.5% T-WDG and 7.5% C-WDG (CORN); 30% or 45% inclusion of T-WDG; and 30% or 45% inclusion of C-WDG. Between CORN, 30% T-WDG, 45% T-WDG, or 45% C-WDG, DMI was not different (P ≥ 0.11), but lambs fed 30% C-WDG had decreased (P ≤ 0.05) DMI compared to other diets. Compared to CORN and 30% T-WDG, DM digestibility was lesser ( P< 0.05) for 45% T-WDG or 30% C-WDG, while 45% C-WDG has lesser (P ≤ 0.05) DM digestibility than all other treatments. Digestibility of NDF was not affected by treatment (P= 0.13), and ADF digestibility was not different ( 0.21) between CORN, 30% T-WDG, 30% C-WDG, or 45% C-WDG. However, digestibility of ADF tended to differ (P = 0.06) between 30% T-WDG and 45% C-WDG and was greater (P ≤ 0.05) in lambs fed 45% T-WDG compared to other treatments. In Exp. 2, 168 steers (421 ± 23.9 kg, SD) were used in a randomized complete block design to determine the impact of C-WDG or T-WDG on growth performance and carcass characteristics. Diets included a corn-based control (CON), 30% T-WDG (TRAD), 30% C-WDG (CEL), and 18% C-WDG and 12% condensed corn distillers solubles (CEL+CCDS; = 7 pens of 6 steers/pen). Steers fed TRAD had improved (P ≤ 0.01) ADG, G:F, and HCW compared to steers fed the CON diet. No differences (P ≥ 0.16) in ADG and HCW were noted for steers fed CEL compared to TRAD; however, steers fed CEL had decreased (P = 0.01) G:F due to increased (P = 0.02) DMI compared to TRAD-fed steers. Steers fed CEL or CEL+CCDS did not differ (P = 0.50) in G:F, but CEL+CCDS-fed steers had lesser (P ≤ 0.01) DMI and ADG likely due to greater S content of the CEL+CCDS diet. Overall, while DM digestibility of lambs fed 30% C-WDG was lesser than 30% T-WDG, performance of steers finished on C-WDG was similar to those fed T-WDG. However, WDG from the secondary fermentation appeared to have lesser energy than T-WDG, while maintaining similar cattle performance to corn-fed controls.

Impact of supplementing vitamin C for 56, 90, or 127 days on growth performance and carcass characteristics of steers fed a 0.31 or 0.59% sulfur diet.

The objective was to examine differential timing of vitamin C (VC) supplementation during the finishing period (for the first 56, 90, or 127 d) on performance, VC, and glutathione (GSH) concentrations and carcass traits of steers receiving a 0.31 or 0.59% S diet. Angus steers (n = 42) were stratified to pens by initial BW (304 ± 13 kg) and GeneMax marbling score (4.3 ± 0.12), and pens were randomly assigned to 1 of 7 treatments (6 steers/pen and 1 pen/treatment), including a high-S (HS; 0.59% S) control (HS CON), HS CON + 10 g VC∙steer(-1)∙d(-1) for the first 56 d of the finishing period, HS CON + 10 g VC∙steer(-1)∙d(-1) for the first 90 d of the finishing period, HS CON + 10 g VC∙steer(-1)∙d(-1) for the entire 127-d finishing period (HS VC127), low-S (LS; 0.31% S) diet + 10 g VC∙steer(-1)∙d(-1) for the first 56 d of the finishing period (LS VC56), LS diet + 10 g VC∙steer(-1)∙d(-1) for the first 90 d of the finishing period, or LS diet + 10 g VC∙steer(-1)∙d(-1) for the entire 127-d finishing period. Jugular blood and ultrasound measures were taken from all steers before feeding on d 0, 56, 90, and 127, and liver biopsies and ruminal hydrogen sulfide measurements were collected on d 121 or 122. Steers (n = 40) were harvested on d 127, and carcass data were collected. Data were analyzed by ANOVA as a completely randomized design with the fixed effect of treatment. Because individual intake data were collected, steer was the experimental unit. Final BW and ADG were greater (P ≤ 0.03) and DMI tended (P = 0.09) to be greater in the LS steers compared to HS steers, but G:F did not differ (P = 0.41) by treatment. A treatment × time effect (P = 0.04) for DMI was noted, likely due to lesser DMI between d 91 and 127 for all treatments except the HS VC127 and LS VC56. Plasma VC concentrations of LS steers were less (P = 0.05) than the HS steers. Total (P = 0.06) and reduced (P = 0.03) plasma GSH were greater in HS steers supplemented with VC than the HS CON, but liver GSH were not different due to S or VC (P ≥ 0.13). The ratio of oxidized to reduced liver GSH was greater (P < 0.01) in HS CON than HS steers supplemented with VC. Marbling score, LM area, KPH, and quality grade were not different (P ≥ 0.19) due to diet, but LS steers had greater (P = 0.05) back fat than HS steers. In conclusion, steers fed a HS diet had poorer live performance and unexpectedly greater plasma ascorbate concentrations than the LS-fed steers. Interestingly, increasing days of VC supplementation across the HS diets increased GSH indices, suggesting that although HS diets may negatively affect antioxidant capacity of cattle, supplementing VC may help correct this.

Effects of duration of vitamin C supplementation during the finishing period on postmortem protein degradation, tenderness, and meat color of the longissimus muscle of calf-fed steers consuming a 0.31 or 0.59% sulfur diet.

High-S (HS) diets have been identified as a causative agent in the development of oxidative stress in cattle, which in postmortem muscle can negatively alter meat quality. Vitamin C (VC) is a potent antioxidant produced endogenously by cattle; however, exogenous supplementation of VC may be useful when HS diets are fed to cattle. The objective of this study was to examine the impact of duration of VC supplementation, for the first 56, 90, or 127 d, during the finishing period on meat color and tenderness of the longissimus thoracis (LT) collected from calf-fed steers consuming a 0.31 or 0.59% S diet. Angus steers ( n= 42) were stratified to pens by initial BW (304 ± 13 kg) and GeneMax marbling score (4.3 ± 0.12), and each pen was randomly assigned to 1 of 7 treatments (6 steers/pen, 1 pen/treatment), including HS (0.59% S, a combination of dried distillers grains plus solubles and sodium sulfate) control (HS CON), HS CON + 10 g VC·steer·(-1)d(-1) for the first 56 d (HS VC56), 90 d (HS VC90), or 127 d (HS VC127), low S (LS; 0.31% S) + 10 g VC·steer·(-1)d(-1) for the first 56 d (LS VC56), 90 d (LS VC90), or 127 d (LS VC127). Steers were harvested (n = 40) and, after a 24-h chill, rib sections (LT) were collected. pH was determined on each rib section before division into 3 sections for determination of 1) 7-d retail display and color and Warner-Bratzler shear force (WBSF), 2) 14-d WBSF determination, and 3) protein degradation and collagen content (2 d postmortem). Data were analyzed by ANOVA as a completely randomized design, with the fixed effect of treatment. Individual feed intake was recorded, and steer was the experimental unit. The HS steers had a greater and lesser percent of the 80- and 76-kDa subunits of calpain-1 (P ≤ 0.05), respectively, and tended to have less (P = 0.08) troponin T degradation (d2), and more (P = 0.02) collagen than LS steers. Increasing days of VC supplementation decreased (P = 0.05) the percentage of the 80 kDa subunit of calpain-1 in HS steers but actually increased it in LS steers (P= 0.003). Supplementing VC, regardless of dietary S, did not affect meat collagen, WBSF, or color (P ≥ 0.12). a* and b* values were greater (P ≤ 0.05) in the LS treatments compared to the HS treatments. Increasing the days of VC supplementation to steers fed a HS diet appears to alleviate the negative effects of the HS diet on calpain-1 but has no effect on muscle tenderness or meat color.

Effect of a multielement trace mineral injection before transit stress on inflammatory response, growth performance, and carcass characteristics of beef steers.

Weaned calves (n = 98; 256 ± 11.5 kg) were used to evaluate the impact of improving trace mineral (TM) status using a multielement TM injection 28 d before transit on markers of inflammatory and stress responses in response to transit and postshipping growth performance. On d 0 of a 28-d preconditioning program, calves received subcutaneous TM injection (MM; n = 48) containing 15, 10, 5, and 60 mg/mL of Cu, Mn, Se, and Zn, respectively, or physiological saline injection (SAL; n = 48). On d 28, steers were weighed, half of the steers from each treatment were transported for a 20-h transit stress period (SHIP; n = 24 per injection treatment), and half of the steers were returned to their pens for 20 h of feed and water restriction without transit (NOSHIP; n = 24 per injection treatment). The SHIP steers were unloaded on d 29 and all steers (SHIP and NOSHIP) were immediately weighed and sorted into new pens (n = 4 steers per pen) for the growing period. At the start of finishing (d 113), steers received a second MM or SAL, resulting in a 2 × 2 × 2 factorial (n = 12 steers per treatment combination). Samples of blood were collected on d 28, 29, and 34 and liver on d 22 and 40. The initial MM increased liver Cu, Se, and Zn concentrations of cattle (P ≤ 0.02) but did not affect ADG during preconditioning (P = 0.89) or BW shrink as a result of transit (P ≥ 0.52). Plasma Fe concentrations were decreased after the transit stress period in SHIP calves (P ≤ 0.05) relative to NOSHIP calves but recovered 5 d after transit, and serum IL-8 concentrations were greater in SAL-SHIP steers than MM-SHIP steers (P = 0.04). Altering TM status through MM caused steers to have lesser ADG (P = 0.03) during the 14-d period after transit (d 29 through 43) but did not affect growth during the growing period (d 5 through 112; P ≥ 0.40). Minimal effects on finishing performance and carcass characteristics were noted, but there was a 3-way interaction (P ≤ 0.02) in which SAL-NOSHIP-MM steers had the greatest yield grade (YG) and smallest ribeye area (REA) and SAL-SHIP-MM steers had the least YG and largest REA. Overall, a MM 28 d before transit or before feed and water restriction did not affect the inflammatory response or plasma TM concentrations but decreased ADG in the 14-d period after transit. Trace mineral injection had limited effects on overall growth performance and carcass characteristics, likely because steer initial TM status was well within the adequate range.