Modelling the innate immune system in microphysiological systems.

This critical review aims to highlight how modeling of the immune response has adapted over time to utilize microphysiological systems. Topics covered here will discuss the integral components of the immune system in various human body systems, and how these interactions are modeled using these systems. Through the use of microphysiological systems, we have not only expanded on foundations of basic immune cell information, but have also gleaned insight on how immune cells work both independently and collaboratively within an entire human body system.

Denial of Personal Racial Discrimination and Its Impact Among People of Color Who Use Substances: Implications for Measuring Racial Discrimination in Substance Use Research.

BACKGROUND: Even though racism is pervasive, some people of color may deny experiencing racial discrimination or may report being unaffected by it. This study examines the contexts and factors that may contribute to these responses among people who use substances. METHODS: We conducted seven focus groups (5-9 participants per group, total N = 43) among Black, Latino, and Asian American adults between the ages of 21 to 44 years old who reported current use of two or more of the following substances: alcohol, cigarettes, e-cigarettes, or cannabis. Data were analyzed using reflexive thematic analysis. RESULTS: Across all three ethno-racial groups, we found some respondents minimized or denied personal experiences of racial discrimination or hesitated to identify their experiences as racial discrimination, which in turn led to respondents to express uncertainty about seeing any sort of connection between racial discrimination and substance use. Themes included a minority comparison effect; a drowning out effect; diversity and racial composition of context; passing as White; and covertness of racism. Also, there were contradictions in accounts, and responses often depended on orienting cues. CONCLUSIONS: While researchers continue to find associations between racial discrimination and substance use, some people of color may not acknowledge this connection. Recommendations include aligning definitions of racism between academic and public/popular discourse; updating measures to keep up with the evolving forms of racism using context-specific examples; combining subjective measures of racial discrimination with objective measures of racism; and dialoguing with the public to raise awareness around how racism is defined.

Modified limited bedding and nesting is a model of early-life stress that affects reproductive physiology and behavior in female and male Long-Evans rats.

We used a modification of the limited bedding and nesting (LBN) model to evaluate the effects of early-life stress (ELS) on female and male reproductive physiology and behavior in Long-Evans rats. On postnatal day (PD) 2, dams and pups were transferred to a cage containing 100 mL of bedding (LBN condition) or to a cage containing 500 mL of bedding (control condition); bedding conditions remained until PD 10. In female rats, we measured vaginal opening, estrous cyclicity, female sexual behavior and motivation, and anxiety-like behavior. In male rats, we measured preputial separation, the development of male copulatory behavior, sexual motivation, and anxiety-like behavior. We found that relative to controls, female rats reared with LBN experienced precocious puberty and enhanced sexual motivation, but normal estrous cyclicity. Relative to controls, male rats reared with LBN experienced delayed puberty and enhanced sexual motivation, but normal development of copulatory behavior. Anxiety-like behavior was not affected by LBN in either female or male rats. In summary, the ELS of being reared with LBN affected the onset of puberty in the opposite direction in females and males, but enhanced sexual motivation in both. The current study is the first to examine the effects of ELS on sexual motivation using the LBN model. These findings further support the hypothesis that maternal care affects the development of sexual maturation and sexual motivation.

The Impact of Early Recognition and Treatment of Systemic Hypertension on Reducing Blood Pressure: The Hypertension and Vascular Evaluation Program.

This study aimed to evaluate the effect of an outpatient systemic hypertension program and associated factors with attending recommended follow-up visit. All visits were tracked in the program, 2011 to 2018. We examined patient characteristics by follow-up status and changes in systolic blood pressure (SBP) and the risk of hypertension in follow-up patients using a mixed-effects regression model. Among 310 patients with first visits, 113 patients returned for a follow-up visit. Patients who did not attend a follow-up were older and less likely to have a severe chronic condition or a family history of hypertension than followed-up patients. The risk of hypertension was significantly reduced by the number of follow-up visits (odds ratio = 0.53, 95% confidence interval = 0.31-0.92). Adolescent SBP and body mass index percentiles decreased with more follow-up visits. As the risk of hypertension is significantly reduced with follow-up visits, additional effort should be made to improve the likelihood of follow-up attendance.