The Protein Phosphatase GhAP2C1 Interacts Together with GhMPK4 to Synergistically Regulate the Immune Response to Fusarium oxysporum in Cotton.

The plant mitogen-activated protein kinase (MAPK) cascade plays an important role in mediating responses to biotic and abiotic stresses and is the main pathway through which extracellular stimuli are transduced intracellularly as signals. Our previous research showed that the GhMKK6-GhMPK4 cascade signaling pathway plays an important role in cotton immunity. To further analyze the role and regulatory mechanism of the GhMKK6-GhMPK4 cascade signaling pathway in cotton resistance to Fusarium wilt, we functionally analyzed GhMPK4. Our results show that silencing GhMPK4 reduces cotton tolerance to Fusarium wilt and reduces the expression of several resistance genes. Further experiments revealed that GhMPK4 is similar to GhMKK6, both of whose overexpression cause unfavorable cotton immune response characteristics. By using a yeast two-hybrid screening library and performing a bioinformatics analysis, we screened and identified a negative regulator of the MAPK kinase-protein phosphatase AP2C1. Through the functional analysis of AP2C1, it was found that, after being silenced, GhAP2C1 increased resistance to Fusarium wilt, but GhAP2C1 overexpression caused sensitivity to Fusarium wilt. These findings show that GhAP2C1 interacts together with GhMPK4 to regulate the immune response of cotton to Fusarium oxysporum, which provides important data for functionally analyzing and studying the feedback regulatory mechanism of the MAPK cascade and helps to clarify the regulatory mechanism through which the MAPK cascade acts in response to pathogens.

The Digital Divide and Active Aging in China.

With the rapid development of society, especially the advent of intelligent technology of life services, the ability of the elderly to adapt to modern digital life is getting weaker and weaker, the dilemma of the "digital divide" for the elderly has aroused heated public debate. In the era of aging and information superposition, in-depth study of the multi-dimensional impact of the digital divide on the elderly has become an effective content of China's active aging strategy. Based on the micro-data of the Chinese General Social Survey in 2017, this paper uses the entropy right method to construct the digital divide index system for the elderly over 60 years of age from the perspective of essential equipment, Internet utilization degree, and Basic Internet usage skills. At the same time, this paper constructs China's comprehensive index of active aging from three aspects: health, social participation, and guarantee of the elderly, to study the impact of the digital divide on China's active aging. The following conclusions have been drawn: the digital divide among the elderly significantly inhibits China's active aging. The digital divide reduces the level of physical and mental health and social participation of older persons and inhibits the level of guarantee of older persons, thus impeding their active aging. In addition, it also reduces the overall life satisfaction of the elderly. The use of the Internet, skills, and other digital technology abilities of the elderly have effectively promoted active aging. The more Internet access devices older people have, the higher their level of social participation. The higher the Internet frequency of the elderly, the healthier the body and mind. Furthermore, the greater the level of physical and mental health and social participation of older groups who use online payments. The digital divide among the elderly inhibits the process of China's active aging, and the unique course and stage characteristics of the development of the aging of the Chinese population require us to pay full attention to the relationship between the digital divide and active aging and how to construct a "digital-friendly" aging system is an essential issue for China's social development to consider.

Molecular and functional characaterization of the novel odorant-binding protein gene AccOBP10 from Apis cerana cerana.

Odorant-binding proteins (OBPs) play an important role in odour perception and transport in insects. However, little is known about whether OBPs perform other functions in insects, particularly in Apis cerana cerana. Within this study, an OBP gene (AccOBP10) was isolated and identified from A. c. cerana. Both homology and phylogenetic relationship analyses indicated that the amino acid sequence of AccOBP10 had a high degree of sequence identity with other members of the gene family. Analysis of quantitative real-time PCR (qRT-PCR) showed that AccOBP10 mRNA was expressed at higher levels in the venom gland than in other tissues. The mRNA transcript expression of AccOBP10 was upregulated by low temperature (4°C), hydrogen peroxide (H2O2), pyridaben, methomyl and imidacloprid but downregulated by heat (42°C), ultraviolet light, vitamin C, mercuric chloride, cadmium chloride, paraquat and phoxim. Expression of AccOBP10 under abiotic stress was analysed by western blotting, and the results were consistent with those of qRT-PCR. And as a further study of AccOBP10 function, we demonstrated that knockdown of AccOBP10 by RNA interference could slightly increase the expression levels of some stress-related genes. Collectively, these results suggest that AccOBP10 is mainly involved in the response to stress conditions.