RESUMO
Two fluorescent probes, 1 and 2, derived from borondipyrromethene (BODIPY) modified with macrocyclic polyamine [12]aneN3, were synthesized and applied in the discrimination of cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) with absorption and fluorescent spectroscopy in comparison. It was found that Boc-protected 1 showed highly sensitive and selective recognition of GSH over Cys and Hcy; while probe 2 was able to distinguish the three different thiols due to their different reactivities. With its water-solubility, rapid responsiveness, high sensitivity and low cytotoxicity, probe 2 was successfully applied in the fast detection of three biothiols in living cells.
Assuntos
Compostos de Boro/química , Corantes Fluorescentes/análise , Corantes Fluorescentes/química , Compostos de Sulfidrila/análise , Compostos de Sulfidrila/química , Sobrevivência Celular , Relação Dose-Resposta a Droga , Células HeLa , Células Hep G2 , Humanos , Estrutura Molecular , Solubilidade , Soluções , Relação Estrutura-Atividade , Células Tumorais Cultivadas , Água/químicaRESUMO
In the title compound, [Na(H(2)O)(4)](C(15)H(9)N(2)O(2)), the Na(+) ion is coordinated by six water mol-ecules in an octa-hedral geometry. The NaO(6) octa-hedra are connected by sharing edges, forming a cationic chain along the b-axis direction. O-Hâ¯O and O-Hâ¯N hydrogen bonds link the chains and the 2-(2-pyrid-yl)quinoline-4-carboxyl-ate anions into a two-dimensional network parallel to (100).
