RESUMO
To date, there has been much disagreement concerning the taxonomic status of Eleutheronema. In this study, the complete mitogenome sequence of Eleutheronema tetradactylum was determined and a Maximum Likelihood (ML) tree was constructed based on concatenated nucleotide sequences of 12 mitochondrial protein-coding genes. The complete mitogenome sequence is 16 490 bp in length, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a putative control region (CR), and a light-strand replication origin (OL). The overall base composition is 27.1% A, 26.0% T, 29.9% C, and 17.0% G, with a slight AT bias (53.1%), similar to most teleostean fishes. All protein-coding genes share the start codon ATG, except for COI, which begins with GTG. Most of them have TAA or TAG as the stop codon, while COII uses AGA, ND4 uses AGG and Cytb uses an incomplete stop codon T--. These results are expected to provide useful molecular data for phylogenetic studies of Polynemidae and Perciformes.
Assuntos
Genoma Mitocondrial , Perciformes/genética , Animais , Composição de Bases , Códon de Iniciação , Códon de Terminação , Proteínas de Peixes/química , Proteínas de Peixes/genética , Funções Verossimilhança , Fases de Leitura Aberta/genética , Perciformes/classificação , Filogenia , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Origem de Replicação/genéticaRESUMO
In this study, two complete mitogenome sequences of Zebrias crossolepis and Acrossocheilus monticola were determined and the phylogenetic relationship were constructed based on concatenated nucleotide sequences of 12 mitochondrial protein-coding genes. The length of the complete mitogenome sequence are 16 775 bp and 16 605 bp in Z. crossolepis and A. monticola, respectively, both containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a putative control region (CR), and a light-strand replication origin (OL). The overall base composition is 28.3% A, 26.3% T, 30.0% C, 15.5% G, with a slight AT bias (54.6%) in Z. crossolepis, while 31.4% A, 24.5% T, 28.2% C, 15.9% G, with an slight AT bias (55.9%) in A. monticola. All the protein-coding genes use the initiation codon ATG except COI uses GTG. Most of them have TAA or TAG as the stop codon, while ND4 and Cytb in Z. crossolepis and COII, ND4, and Cytb in A. monticola use an incomplete stop codon T. These results are expected to provide useful molecular data for species identification and further phylogenetic studies.
Assuntos
Cipriniformes/genética , DNA Mitocondrial/genética , Linguados/genética , Genoma Mitocondrial/genética , Animais , Composição de Bases/genética , Sequência de Bases/genética , Sequência Conservada/genética , Cyprinidae/genética , Ordem dos Genes/genética , Genes Mitocondriais/genética , Mitocôndrias/genética , Filogenia , Análise de Sequência de DNA/métodos , Sequenciamento Completo do GenomaRESUMO
At present, the phylogenetic and taxonomic positions of Bagridae and Siluriformes have long been unclear. In this paper, we sequenced and annotated the complete mitogenome of Hemibagrus sp. The total length was 16,529 bp, and was typically consist of 37 genes, including 13 protein-coding genes, 2 rRNAs, 22 tRNA, a light-strand replication origin (OL) and a single large control region (CR). Except for eight tRNA and ND6 genes, all other mitochondrial genes were encoded on the heavy strand (H-strand). The gene content, organization and arrangement were identical to that of a typical bony fish. The overall base composition was 26.5%, 26.8%, 31.7% and 15.0% for T, C, A and G, respectively, with a slight bias on AT content (58.2%). This information could not only contribute to provide useful molecular data for the species identification, but also to further taxonomic and phylogenetic studies of Hemibagrus and Bagridae.
Assuntos
Peixes-Gato/genética , Genoma Mitocondrial , Sequenciamento Completo do Genoma , Animais , Composição de Bases/genética , Pareamento de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , DNA Ribossômico/genética , Genes Mitocondriais , RNA de Transferência/genéticaRESUMO
PCR-SSCP technology was used to analyze the correlation of polymorphisms IGF2 (Insulin-like growth factor) gene with carcass and meat quality traits in 186 Qinchuan cattle at the age of 24-month. C-->T mutation in 120 and A-->G mutation in 279 of IGF2 gene. Statistical analysis indicated that Qinchuan cattle with genotype BB and DD had significant differences in slaughter weight, carcass weight, carcass length, carcass chest depth and eye muscle area (P<0.05). The difference in thickness of back fat was significant (P<0.01). Significant differences in marbling, tenderness, pH24 of meat quality (P<0.05), but the differences in carcass depth and water holding capacity were not significant (P>0.05). Genotype AA, DD were predominant genotypes and A, D were predominant alleles. The population containing B, D allele had more excellent carcass and meat quality than others, especially in the capacity of fat accumulate.
Assuntos
Peso Corporal/genética , Bovinos/genética , Fator de Crescimento Insulin-Like II/genética , Carne/normas , Polimorfismo de Nucleotídeo Único/genética , Animais , Genótipo , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples/genética , Análise de Sequência de DNARESUMO
The complete sequences of mitochondrial DNA D-loop of 140 individuals in 10 Chinese goat (Capra hiruc) breeds were analyzed by DNA sequencing technology. The results showed that the length of mtDNA D-loop in Chinese goats was 1 211-1 213 bp. There were 84 different haplotypes and 171 polymorphic sites. The mean nucleotide diversity (Pi) and haplotype diversity (Hd) were 0.02063+/+0.00225 and 0.988+/-0.003, respectively, and the average number of nucleotide differences (k) was 24.896. The results showed an abundant genetic diversity in domestic goats of China. The NJ tree indicated that there were two main branches in Chinese domestic goats, thereinto, one branch was clustered with Capra aegagrus, and Capra falconeri was clustered alone, which indicated that Capra aegagrus had more contribution to Chinese domestic goat breeds.
