[Melatonin secretion and metabolism in patients with irritable bowel syndrome]. / Wydzielanie i metabolizm melatoniny u choryc z zespolem jelita nadwrazliwego.

UNLABELLED: Melatonin possesses enteroprotective proprieties. An action of a gastrointestinal motility modulator is also attributed to this hormone. Clinical investigations have proven that melatonin plays a role mainly in the pathogenesis of upper gastrointestinal tract disorders, much less data have been published on its action in intestinal disorders. AIM OF THIS STUDY: To evaluate melatonin (MEL) secretion and its metabolism to 6-hydroxymelatonin sulfate (6HMS) in patients with irritable bowel syndrome (IBS). MATERIAL AND METHODS: 75 subjects, aged 18-52 years were included in this study. Three groups were distinguished: K (n=25)--healthy volunteers, IBS-D (n=25)--patients with diarrhoea predominant IBS, IBS-C (n=25)--subjects with constipation predominant IBS (IBS-C). IBS was diagnosed according to Rome III Criteria. Blood samples were taken from cubital vein at 2:00 a.m. and 8:00 a.m. on the next day. Concentrations of MEL in serum and 6HMS in 24-hour urine collection were determined by immunoenzyme ELISA method employing standard kits IBL nr RE59121 i RE59131). RESULTS: The average melatonin concentration at 2:00 a.m. was found to be in the studied groups: K-55.3 +/- 6,6 pg/ml, IBS-D--57.5 +/- 16.7 pg/ml (p > 0.05), IBS-C--75.5 +/- .3 pg/ml (p < 0.01) and at 8:00 a.m. adequately--7.3 +/- 4.0 pg/ml, 14.9 +/- 8.9 pg/ml (p < 0.001) and 15.1 +/- 7.2 pg/ml (p < 0.001). 24-hour urinary 6HMS excretion amounted to: K--15.10 +/- 6.37 microg/ 24h, IBS-D--27.67 +/- 26.71 microg/24h (p < 0.05), IBS-C--31.47 +/- 29.19 microg/24h (p < 0.01). CONCLUSION: Melatonin secretion in patients with IBS, especially in patients with IBS-C, is significantly increased compared with healthy volunteers. Melatonin metabolism in patients with IBS is not disturbed.

[24-hour urinary 5-hydroxyindole acetic acid excretion in patients with ulcerative colitis]. / Wydalanie kwasu 5-hydroksyindolooctowego z moczem u osób z wrzodziejacym zapaleniem jelita grubego.

UNLABELLED: The experimental data show that in mucosal injury of the alimentary system serotonin metabolism and secretion is disturbed. Such suggestions are also drawn in discussions concerning pathogenesis of ulcerative colitis. THE AIM OF THE STUDY: To perform a quantitative evaluation of 24-hour urine excretion of a major serotonin metabolite-5-hydroxyindole acetic acid (5-HIAA) in patients with ulcerative colitis (UC). MATERIAL AND METHODS: The studied group comprised 50 patients with exacerbated UC, aged 21-56 years. Among them 25 had mild and 25 severe exacerbation according to Trulove's and Witts criteria. The control group comprised 25 healthy persons. On the study day the patients remained on standard liquid diet--Nutrdrink 3 x 400 ml with the caloric value of 1800 kcal. 5-HIAA concentration was measured by ELISA method applying IBL antibodies (RE 59131), and the results were presented in mg/dl and then the value of 24-hour urine excretion was calculated. Results. In the group o patients with severely exacerbated UC 5-HIAA excretion was significantly lower that in healthy volunteers--1.66 +/- 0.98 mg/24 hi 5.77 +/- 0.46 mg/24 respectively (p < 0.05). The results of 5-HIAA excretion showed positive correlation with serum albumin concentration. In the group of patients with mild colitis such significant changes and correlations were not observed. CONCLUSIONS: (1) In severe exacerbations of UC serotonin metabolism is largely changed. (2) 24-hour serotonin excretion can have a predictive value in UC.

[The assessment of selected personality traits, coping and melatonin nocturnal secretion in patients with functional dyspepsia]. / Ocena wybranych cech osobowosci, stylów radzenia sobie ze stresem oraz nocnego wydzielania melatoniny u chorych z dyspepsjq czynnosciowa.

AIM: The main aim of the research was a characteristic of selected personality traits and coping as well as estimation of a level of melatonin in serum in patients with functional dyspepsia (FD). METHODS: 36 patients with FD (14 men and 24 women) at age 19-43 (mean: 31.6) were examined. The control group consisted of 30 healthy persons at age 21-23 (mean: 37.2). CO-16 and CISS questionnaires were used to diagnose selected traits of personality and coping styles. Furthermore, melatonin concentration in serum was examined at 10 p.m., 2 a.m. and at 6 a.m. with the immunoenzymatic method (ELISA). RESULTS: Coping style focused on problems and emotions were the most frequent ones in the examined group. Cyclothymia, tendency towards neuroticism and depression, submission and sensitivity were these that characterised patients with FD well. It was also stated that the level of melatonin was higher than in healthy subjects. CONCLUSIONS: There are common personality traits and coping styles in the group of patients with FD. A level of melatonin in serum is increased.

[The influence of melatonin on concentration of nitric oxide metabolites in gastric juice in subjects with functional dyspepsia]. / Wplyw melatoniny na stezenie metabolitów tlenku azotu w soku zoladkowym u osób z dyspepsja czynnosciowa.

UNLABELLED: The pathogenesis of functional dyspepsia is very complicated and its etiology is still not clear. One of the supposed pathophysiological mechanisms is the deficiency of melatonin. The deficiency of melatonin leads to increase the oxide reactive form's concentration as nitric oxide metabolites and to decrease of antyoxidative enzymes activity. This last factor seems to be very important in correct digestive tract function. The aim of our study was answer the question if is the difference between NO metabolites concentraction in gastric juice in patients with functional dyspepsia and in healthy subjects and wheather the treatment with melatonin plays the role in normal digestive tract function. MATERIAL AND METHODS: The study included 60 subjects between of 18 to 48 years with diagnosed functional dyspepsia (according to the Rome III Criteria). The study group was divided into two subgroups: group I--30 subjects with Epigastric Pain Syndrome-EPS and group II--30 subjects with Postprandial Disorders Syndrome-PDS. Control group comprised 25 healthy subjects (without any clinical or morphological symptoms of digestive tract disease). In each patient the gastroscopy was performed. During gastroscopy 5 ml gastric juice was collected. The juice was centrifuged for 15 min (4500 rotations). The undiluted supernatant was frozen in the temperature -70 degrees C. The concentration of nitric oxide metabolites in gastric juice was determined with spectrophotometric based on ELISA test (540 nm wavelength) using a microplates reader (Multiscan, Labsystems). In patients with functional dyspepsja the investigations were performer twice before and after 6 weeks treatment with melatonin. Melatonin was applied in dose 5 mg daily, in the evening. RESULTS: The concentration of nitric oxide metabolites in gastric juice in healthy subjects was 6.81 +/- 2.23 microM. In patients with functional dyspepsia was significantly higher; in patients with Epigastric Pain Syndrome--10.99 +/- 2.46 microM (p < 0.05), in patients with Postprandial Disorders Syndrome--9.28 +/- 2.18 microM (p < 0.05). After treatment with melatonin the concentration of nitric oxide metabolites in gastric juice in both groups decreased and were 8.21 +/- 1.83 microM in patients with Epigastric Pain Syndrome and 6.93 +/- 1.61 microM in patients with Postprandial Disorders Syndrome. CONCLUSIONS: In patients with functional dyspepsia the concentration of nitric oxide metabolites in gastric juice was significantly higher than in control group. After treatment with melatonin the concentration of nitric oxide metabolites in gastric juice in both groups decreased--in patients with Epigastric Pain Syndrome as well as in patients with Postprandial Disorders Syndrome. The treatment with melatonin seems to be suitable in combined therapy of functional dyspepsia.

[Secretion of serotonin and melatonin in patients with functional dyspepsia]. / Wydzielanie serotoniny i melatoniny u osáb z dyspepsja czynnosciowa.

UNLABELLED: It was confirmed that enterochromaffin cells (EC) in the digestive system are a source of serotonin and melatonin. Both hormones possess an important, but opposed, influence on secretory and motoric function of gut. The aim of our study was to evaluate fasting secretion of serotonin and melatonin in patients with functional dyspepsia. Material and methods. 25 healthy subjects (K, group I), 25 patients with epigastric pain syndrome (EPS, group II) and 25 patients with postprandial distress syndrome (PDS, group III), aged 18-50 years, were included in this study. Diagnosis of functional dyspepsia was established according to the Rome Criteria III. Organic diseases of gut, H. pylori infection and another disorders were excluded. Seven days before the examinations the patients were told to abstain from drugs intake and equal diet was applied. On the day of investigation the subjects remained in a red-light room at night and received standard liquid diet (Nutridrink, 4 x 200 ml, 1800 kcal). Blood samples were taken for examination at 8 am on the subsequent day and serum was frozen in -80 degrees C. The concentration of serotonin and melatonin was measured by ELSA, using antibodies from IBL (catalogue no. RE 59121, RE 54021). RESULTS: The mean concentration of serotonin in group I (K) was 162.9 +/- 49.1 ng/ml, in group II (EPS)--225.8 +/- 111.3 ng/ml (p < 0.05) and in group III (PDS)--152.7 +/- 83.2 ng/ml (p > 0.05). The concentration of melatonin was in group I--7.3 +/- 4.0 pg/ml, in group II 8.7 +/- 8.0 (p > 0.05) and in group III 14.3 +/- 10.1 (p < 0.01). CONCLUSIONS: During fasting time secretion of serotonin is higher in patients with EPS, and similarly higher secretion of melatonin in patients with PDS, compared to healthy subjects.

Therapeutic effect of melatonin in patients with functional dyspepsia.

BACKGROUND AND GOAL: Melatonin may inhibit reactive oxygen species-related pathogenesis in the alimentary tract by neutralizing free radicals. In the present study we assessed the potential protective action of melatonin in ulcerlike dyspepsia. STUDY: Sixty patients aged 19 to 39 years with the diagnosis of functional dyspepsia according to the Rome Criteria II and no Helicobacter pylori infection were involved in the study. Melatonin, at a dose of 5 mg (n=30), or placebo (n=30) were taken in the evening for a period of 12 weeks. At this time, patients were on an equivalent diet and were only to take an alkaline drug in case of the abdominal pain. RESULTS: After 12 weeks, the dyspeptic symptoms completely subsided in 17 patients in the melatonin-treatment group (56.6%). In other 9 individuals (30.0%) a partial improvement in health was achieved, especially in the frequency and intensity of nocturnal pain. After placebo, the majority of patients (93.3%) did not experience any improvement in symptoms. Multivariate analysis indicated that melatonin (odds ratio 95.86, 95% confidence interval 3.72-2469.37, P<0.01) correlated independently with significantly improved patients health. H. pylori past infection decreased positive effect of melatonin in ulcerlike dyspepsia. CONCLUSIONS: Melatonin can be considered as an auxiliary drug in the treatment of ulcerlike dyspepsia.

[Nocturnal secretion of melatonin in subjects with asymptomatic and symptomatic Helicobacter pylori infection]. / Nocne wydzielanie melatoniny u pacjentów z bezobjawowym i objawowym zakazeniem Helicobacter pylori.

UNLABELLED: Melatonin is synthesized not only in the pineal gland, but also in gastrointestinal tract by enterochromaffin cells (EC) and it has a gastroprotective properties. Helicobacter pylori infection causes functional and organic changes of gastric mucosa, particularly in endocrine cells (D, G, ECL, EC). AIM: The aim of own studies was to evaluate nocturnal secretion of Melatonin in subjects with asymptomatic and symptomatic (dyspepsia, duodenal ulcer disease) H. pylori infection (Hp). MATERIAL AND METHODS: 116 subjects, aged 19-61 years were included in this study. Four groups were distinguished: group I (n=30) healthy subjects, Hp (-), group II (n=26)--subjects with asymptomatic infection Hp, group III (n=32) patients with ulcer-like dyspepsia, Hp (+), and group IV (n=28) patients with duodenal ulcer Hp(+). H. pylori infection was diagnosed by using urea breath test (UBT-(13)C) and rapid urease test. The concentration of melatonin in serum was measured with ELISA method, before and after eradication of H. pylori. Blood samples were taken for examination at 10:00 p.m., 2:00 a.m. and 6:00 a.m. RESULTS: The average melatonin concentration at night hours was found in group I--34.74 +/- 4.77 pg/ml, in group II--48.29 +/- 12.22 pg/ml (p < O.005), in group III--41.56 +/- 12.31 pg/ml (p > 0.05) and in group IV--26.16 +/- 6.89 pg/ml p < 0.01). Such statistical differences were not observed in all group after eradication H. pylori. CONCLUSIONS: (1) Nocturnal secretion of melatonin in subjects with asymptomatic infection of H. pylori is higher than in patients with ulcer-like dyspepsia and with duodenal ulcer disease. (2) Lower nocturnal secretion of melatonin probably may play role in pathogenesis of upper digestive tract diseases.